Altered mRNA expression patterns in bovine blastocysts after fertilisation in vitro using flow‐cytometrically sex‐sorted sperm

Molecular Reproduction and Development - Tập 74 Số 8 - Trang 931-940 - 2007
KM Morton1, D. Herrmann2, B. Sieg2, C. Struckmann2, W.M.C. Maxwell1, D. Rath2, G. Evans1, Andrea Lucas‐Hahn2, Heiner Niemann2, C. Wrenzycki2
1Centre for Advanced Technologies in Animal Genetics and Reproduction (ReproGen), Faculty of Veterinary Science, The University of Sydney, Australia
2Department of Biotechnology, Institute for Animal Breeding (FAL), Neustadt, Germany

Tóm tắt

AbstractSperm‐sexing has been used to produce embryos and offspring of a pre‐determined sex in a number of species. However, the fertility of sex‐sorted sperm is reduced and the full effects of sperm‐sexing remain to be elucidated. The purpose of the present study was to investigate the potential effects of sex‐sorted sperm on mRNA expression patterns of developmentally important genes employing in vitro produced bovine embryos. Bovine embryos were produced in vitro with unsorted and sex‐sorted sperm and mRNA expression patterns were determined for glucose‐3 transporter (Glut‐3), glucose‐6‐phosphate dehydrogenase (G6PD), X‐inactive specific transcript (X‐ist) and Heat shock protein 70.1 (Hsp) using semi‐quantitative endpoint reverse transcriptase‐PCR in male and female, day‐7 and 8 embryos. The relative abundance (RA) of Glut‐3 was higher for day‐7 male than female embryos, and day‐7 embryos derived from unsorted compared with sex‐sorted sperm. The RA of G6PD was higher for embryos derived from unsorted than sex‐sorted sperm, and for day‐8 female compared with male embryos. The RA of Xist was higher for female than male embryos, and for day‐7 female embryos derived from unsorted than sex‐sorted sperm. Hsp RA was higher for female compared with male embryos, was similar for day‐7 and 8 embryos, and unsorted and sex‐sorted sperm derived embryos. These results demonstrate differential expression of developmentally important genes between male and female embryos, and embryos derived from unsorted and sex‐sorted sperm. Mol. Reprod. Dev. 74: 931–940, 2007. © 2007 Wiley‐Liss, Inc.

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Molecular Reproduction and Development

Tập 74 Số 8

931-940

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