A large proportion of asymptomatic Plasmodium infections with low and sub-microscopic parasite densities in the low transmission setting of Temotu Province, Solomon Islands: challenges for malaria diagnostics in an elimination setting Tập 9 Số 1 - 2010
Ivor Harris, Wesley W Sharrock, Lisa Bain, Karen-Ann Gray, Albino Bobogare, Leonard Boaz, Ken Lilley, Darren Krause, Andrew Vallely, Marie-Louise Johnson, Michelle L. Gatton, G. Dennis Shanks, Qin Cheng
Abstract Background Many countries are scaling up malaria interventions towards
elimination. This transition changes demands on malaria diagnostics from
diagnosing ill patients to detecting parasites in all carriers including
asymptomatic infections and infections with low parasite densities. Detection
methods suitable to local malaria epidemiology must be selected prior to
transitioning a malaria... hiện toàn bộ
Designing the next generation of medicines for malaria control and eradication - 2013
Jeremy N. Burrows, Rob Hooft van Huijsduijnen, Jörg J. Möhrle, Claude Oeuvray, Timothy N.C. Wells
Abstract In the fight against malaria new medicines are an essential weapon. For
the parts of the world where the current gold standard artemisinin combination
therapies are active, significant improvements can still be made: for example
combination medicines which allow for single dose regimens, cheaper, safer and
more effective medicines, or improved stability under field conditions. For
those p... hiện toàn bộ
A general SNP-based molecular barcode for Plasmodium falciparum identification and tracking Tập 7 Số 1 - 2008
Rachel F. Daniels, Sarah K. Volkman, Danny A. Milner, Nira Mahesh, Daniel E. Neafsey, Daniel J. Park, David Rosen, Elaine Angelino, Pardis C. Sabeti, Dyann F. Wirth, Roger C. Wiegand
Abstract Background Single nucleotide polymorphism (SNP) genotyping provides the
means to develop a practical, rapid, inexpensive assay that will uniquely
identify any Plasmodium falciparum parasite using a small amount of DNA. Such an
assay could be used to distinguish recrudescence from re-infection in drug
trials, to monitor the frequency and distribution of specific parasites in a
patient popu... hiện toàn bộ
Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species Tập 7 Số 1 - 2008
Clotilde Ribaut, Antoine Berry, Séverine Chevalley, Karine Reybier, Isabelle Morlais, Daniel Parzy, Françoise Nepveu, Françoise Benoit‐Vical, Alexis Valentin
Abstract Background Parasite concentration methods facilitate molecular,
biochemical and immunological research on the erythrocytic stages of Plasmodium.
In this paper, an adaptation of magnetic MACS® columns for the purification of
human Plasmodium species is presented. This method was useful for the
concentration/purification of either schizonts or gametocytes. Results and
conclusions The magnet... hiện toàn bộ
Clustered local transmission and asymptomatic Plasmodium falciparum and Plasmodium vivax malaria infections in a recently emerged, hypoendemic Peruvian Amazon community Tập 4 Số 1 - 2005
OraLee H. Branch, W. Martin Casapia, Dionicia Gamboa, Jean N. Hernandez, Freddy Alava, Norma Roncal, Eugenia Alvarez, Enrique J. Martínez Pérez, Eduardo Gotuzzo
Abstract Background There is a low incidence of malaria in Iquitos, Peru,
suburbs detected by passive case-detection. This low incidence might be
attributable to infections clustered in some households/regions and/or
undetected asymptomatic infections. Methods Passive case-detection (PCD) during
the malaria season (February-July) and an active case-detection (ACD)
community-wide survey (March) sur... hiện toàn bộ