Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species

Malaria Journal - Tập 7 Số 1 - 2008
Clotilde Ribaut1, Antoine Berry2, Séverine Chevalley1, Karine Reybier1, Isabelle Morlais3, Daniel Parzy4, Françoise Nepveu1, Françoise Benoit‐Vical2, Alexis Valentin1
1Université de Toulouse, Laboratoire Pharmacochimie des Substances Naturelles et Pharmacophores Redox, UMR 152 IRD-Université, Université Paul Sabatier, Faculté de Pharmacie, 118 route de Narbonne, 31062, Toulouse Cedex 9, France
2Université de Toulouse, Service de Parasitologie et Mycologie, Hôpital Rangueil-Larrey CHU de Toulouse et Faculté de Médecine de Rangueil, 1 av. Jean Poulhes – TSA 50032, 31059, Toulouse cedex 9, France
3Laboratoire d'Entomologie Médicale, OCEAC-IRD, BP288 Yaoundé, Cameroon
4Unité de Parasitologie, IMTSSA, 13998, Marseille Armées, France

Tóm tắt

Abstract Background

Parasite concentration methods facilitate molecular, biochemical and immunological research on the erythrocytic stages of Plasmodium. In this paper, an adaptation of magnetic MACS® columns for the purification of human Plasmodium species is presented. This method was useful for the concentration/purification of either schizonts or gametocytes.

 Results and conclusions

The magnetic removal of non-parasitized red blood cells (in vivo and in vitro) using magnetic columns (MACS) was evaluated. This easy-to-use technique enriched schizonts and gametocytes from Plasmodium falciparum in vitro cultures with a very high degree of purity. In addition, all haemozoin-containing stages (schizonts and/or gametocytes) from the peripheral blood of infected patients could be concentrated using this method. This method is particularly useful for the concentration of non-falciparum species, which do not grow in culture and are otherwise difficult to obtain in large amounts.

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Malaria Journal

Tập 7 Số 1

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