Journal of Protein Chemistry

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A Monomeric Mannose-Binding Lectin from Inner Shoots of the Edible Chive (Allium tuberosum)
Journal of Protein Chemistry - Tập 20 - Trang 361-366 - 2001
Y. W. Lam, T. B. Ng
A mannose-binding lectin was isolated from the inner shoots of the chive Allium tuberosum. The procedure involved aqueous extraction, (NH4)2SO4 precipitation, dialysis to remove (NH4)2SO4, affinity chromatography on mannose-agarose, ion exchange chromatography on SP-Sepharose, gel filtration on Superdex 75, and ion exchange chromatography on Mono S. Lectin activity was adsorbed on mannose-agarose,...... hiện toàn bộ
Affinity labeling of the active site of pig liver NADH-cytochrome b5 reductase by 5′-p-fluorosulfonylbenzoyladenosine
Journal of Protein Chemistry - Tập 5 - Trang 133-145 - 1986
Shiuan Chen, Mitsuru Haniu, Takashi Iyanagi, John E. Shively
Lysosome-solubilized pig liver NADH-cytochrome b5 reductase is inactivated by 5′-p-fluorosulfonylbenzoyladenosine (5′-FSBA) following pseudo-first-order kinetics. A double reciprocal plot of 1/Kobs versus 1/[5′-FSBA] yields a straight line with a positiveY intercept, indicative of reversible binding of the analogue prior to an irreversible incorporation.Kdor the initial reversible enzyme-analogue ...... hiện toàn bộ
Probing the Roles of the Only Universally Conserved Leucine Residue (Leu122) in the Oligomerization and Chaperone-like Activity of Mycobacterium tuberculosis Small Heat Shock Protein Hsp16.3
Journal of Protein Chemistry - - 2000
Hongzheng Dai, Qilong Mao, Wenxian Yang, Sufang Huang, Zengyi Chang
Assignment of the Disulfide Bridges in Bothropstoxin-I, a Myonecrotic Lys49 PLA2 Homolog from Bothrops jararacussu Snake Venom
Journal of Protein Chemistry - Tập 20 - Trang 377-382 - 2001
Adélia C. O. Cintra, Suely V. Sampaio, Arni K. Raghuvir, José R. Giglio
Bothropstoxin-I (BthTX-I), a Lys49 phospholipase A2 homolog with no apparent catalytic activity, was first isolated from Bothrops jararacussu snake venom and completely sequenced in this laboratory. It is a 121-amino-acid single polypeptide chain, highly myonecrotic, despite its inability to catalyze hydrolysis of egg yolk phospholipids, and has 14 half-cystine residues identified at positions 27,...... hiện toàn bộ
MPSA short communications
Journal of Protein Chemistry - Tập 13 - Trang 431-512 - 1994
Eugene M. Barnes, Patricia A. Calkin, Satoshi Kuroda, Shigemi Norioka, Masanori Mitta, Ikunoshin Kato, Fumio Sakiyama, Heinz Nika, David T. Chow, Daniel Hess, Edward J. Bures, Hamish D. Morrison, Ruedi Aebersold, G. Marius Clore, Angela M. Gronenborn, Bengt Persson, Patrick Argos, Peter James, Andrew C. Cannons, Larry P. Solomonson, Kenneth E. Dombrowski, William E. Moddeman, Stephen E. Wright, Winona C. Barker, David G. George, Subhendra N. Mattagajasingh, Hara P. Misra, Shuan Shian Huang, Jung San Huang, Y. C. Lee, Wolfgang H. Fischer, A. Grey Craig, Philip N. McFadden, Jonathan A. Lind-quist, M. Bartlet-Jones, W. Jeffery, H. F. Hansen, D. J. C. Pappin, Tomas Bergman, Lars Hjelmqvist, Mats Estonius, Hans Jörnvall, Donna S. Dorow, H. Tschesche, V. Knäuper, T. Kleine, P. Reinemer, S. Schnierer, F. Grams, W. Bode, Christopher Southan, Kenneth Fantom, Patric Lavery, J. B. C. Findlay, D. Akrigg, T. K. Attwood, M. J. Beck, A. J. Bleasby, A. C. T. North, D. J. Parry-Smith, D. N. Perkins, A. Aitken, Y. Patel, H. Martin, D. Jones, K. Robinson, J. Madrazo, S. Howell, Tom Yungwirth, Michael Affolter, Lawrence Amankwa, Harold A. Scheraga, Chao -Yuh Yang, Natalia V. Valentinova, Manlan Yang, Zi -Wei Gu, Antonio M. Gotto, Norman J. Dovichi, Karen C. Waldron, Min Chen, Ian Ireland, Akira Omori, Sachiyo Yoshida, Johann Schaller, Stephan Lengweiler, Egon E. Rickli, José Bubis, Julio O. Ortiz, Carolina Möller, Enrique J. Millán, Victoria L. Boyd, MeriLisa Bozzini, Jindong Zhao, Robert J. DeFranco, Pau -Miau Yuan, G. Marc Loudon, Duy Nguyen, Masaharu Kamo, Takao Kawakami, Norifumi Miyatake, Akira Tsugita, Keiji Takamoto, Kazuo Satake, Oliver Bischof, Mirko Hechenberger, Bernd Thiede, Volker Kruft, Brigitte Wittmann-Liebold, Albrecht Otto, Rainer Benndorf, Peter Jungblut, Monika Ühlein, Henning Urlaub, Rita Berhardt, Regine Kraft, Heike Uhlmann, Vita Beckert, Toshifumi Akizawa, Takaaki Ayabe, Motomi Matsukawa, Michiyasu Itoh, Masatoshi Nishi, Hiroshi Sato, Motoharu Seiki, Masanori Yoshioka, Michal Lebl, Viktor Krchňák, Nikolai F. Sepetov, Petr Kočiš, Marcel Pátek, Zuzana Flegelová, Ronald Ferguson, Kit S. Lam, Robert L. Moritz, James Eddes, Hong Ji, Gavin E. Reid, Richard J. Simpson, William Seffens, C. Dale Poulter, Julia M. Dolence, Pamela D. Bond, Kiyoshi Nokihara, Kazuo Ikegaya, Naoki Morita, Takao Ohmura, S. I. Salikhov, N. J. Sagdiev, A. S. Korneev, Behzod Z. Dolimbek, M. Zhouhair Atassi, J. S. Rosenberg, Z. Yun, P. R. Wyde, M. Z. Atassi, Simon J. Gaskell, Kalyan Rao Anumula, David P. Goldenberg, Ettore Appella, Michelle Fiscella, Nicola Zambrano, Stephen J. Ullrich, Kazuyasu Sakaguchi, Hiroshi Sakamoto, Marc S. Lewis, David Lin, W. Edward Mercer, Carl W. Anderson, Marjorie A. Connelly, Hong Zhang, John D. Sipley, Susan P. Lees-Miller, Stephen P. Jackson, Yong-hong Xie, Jun A. Quion, Chao-yuh Yang, W. F. Brandt, H. Alk, R. Bhaskaran, Chin Yu, C. C. Yang, Agnes H. Henschen, Keith Ashman, Matthias Mann, Juan Guevara, Hung Michael Nguyen, Daniel B. Davison, Joel D. Morrisett, Richard N. Perham, Donald A. Marvin, Martyn F. Symmons, Tamsin D. Terry, Z. H. Beg, J. A. Stonik, J. M. Hoeg, H. B. Brewer, Boris M. Gorovits, C. S. Raman, Paul M. Horowtiz
Aggregate Formation and the Structure of the Aggregates of Disulfide-Reduced Proteins
Journal of Protein Chemistry - Tập 21 - Trang 427-433 - 2002
Kenji Takase, Takahiko Higashi, Toshihiro Omura
Aggregate formation and the structure of the aggregates of disulfide-reduced proteins were investigated using α-lactalbumin and lysozyme as model proteins. First, reducing conditions were adjusted so that only one of the four disulfide bonds present in each native protein was cleaved. These three-disulfide (3SS) proteins are known to adopt almost native conformations, yet formed precipitates with ...... hiện toàn bộ
Histone-poly(A) hybrid molecules as tools to block nuclear pores
Journal of Protein Chemistry - - 1995
Gernot Cremer, Eva Wojtech, Matthias Kalbas, Paul S. Agutter, Detlef Prochnow
Properties of Cys21-mutated muscle acylphosphatases
Journal of Protein Chemistry - Tập 15 Số 1 - Trang 27-34 - 1996
Modesti, A., Taddei, N., Chiti, F., Bucciantini, M., Magherini, F., Rigacci, S., Stefani, M., Raugei, G., Ramponi, G.
Cys21 is an invariant residue in muscle acylphosphatases, but is absent in the erythrocyte isozymes. To assess the importance of this residue in the muscle isozymes for catalytic, structural, and stability properties, two gene mutants have been prepared by oligonucleotide-directed mutagenesis and expressed inEscherichia coli cells; in these mutants, the codon for Cys21 was replaced by those for Se...... hiện toàn bộ
In vitro preparation and partial characterization of a novel complex of human fibrinogen with albumin
Journal of Protein Chemistry - Tập 14 - Trang 259-262 - 1995
Boguslaw Lipinski
Limited reduction of a mixture of purified human fibrinogen and human serum albumin leads to the formation of a stable complex. This complex is clottable with thrombin, but its electrophoretic mobility is lower than that of fibrinogen. By contrast to clots prepared from purified fibrinogen, those obtained from the complex are transparent and are completely resistant to fibrinolytic degradation ind...... hiện toàn bộ
A computer modeling postulated mechanism for angiotensin II receptor activation
Journal of Protein Chemistry - - 1995
Marie-Pierre Joseph, Bernard Maigret, Jean‐Claude Bonnafous, Jacky Marie, Harold A. Scheraga
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