Genesis
SCIE-ISI SCOPUS (2000-2025)
1526-954X
1526-968X
Mỹ
Cơ quản chủ quản: WILEY , Wiley-Liss Inc.
Lĩnh vực:
EndocrinologyGeneticsMedicine (miscellaneous)Cell Biology
Phân tích ảnh hưởng
Các bài báo tiêu biểu
A global double‐fluorescent Cre reporter mouse
Tập 45 Số 9 - Trang 593-605 - 2007
AbstractThe Cre/loxP system has been used extensively for conditional
mutagenesis in mice. Reporters of Cre activity are important for defining the
spatial and temporal extent of Cre‐mediated recombination. Here we describe
mT/mG, a double‐fluorescent Cre reporter mouse that expresses membrane‐targeted
tandem dimer Tomato (mT) prior to Cre‐mediated excision and membrane‐targeted
green fluorescent ...
Expression of Cre recombinase in the developing mouse limb bud driven by a Prxl enhancer
Tập 33 Số 2 - Trang 77-80 - 2002
AbstractSummary: We have used a Prx1 limb enhancer to drive expression of Cre
Recombinase in transgenic mice. This regulatory element leads to Cre expression
throughout the early limb bud mesenchyme and in a subset of craniofacial
mesenchyme. Crossing a murine line carrying this transgene to a reporter mouse
harboring a floxed Cre‐reporter cassette revealed that recombinase activity is
first obser...
hGFAP‐cre transgenic mice for manipulation of glial and neuronal function in vivo
Tập 31 Số 2 - Trang 85-94 - 2001
AbstractWith the goal of performing astrocyte‐specific modification of genes in
the mouse, we have generated a transgenic line expressing Cre recombinase under
the control of the human glial fibrillary acidic protein (hGFAP) promoter.
Activity was monitored by crossing the hGFAP‐cre transgenics with either of two
reporter lines carrying a lacZ gene whose expression requires excision of
loxP‐flanke...
Genetic targeting of principal neurons in neocortex and hippocampus of NEX-Cre mice
Tập 44 Số 12 - Trang 611-621 - 2006
Consequences of knocking out BMP signaling in the mouse
Tập 35 Số 1 - Trang 43-56 - 2003
AbstractSummary: During the past two decades, a significant amount of data has
been accumulated revealing the intriguing functions of bone morphogenetic
proteins (BMPs) in all aspects of embryonic development and organogenesis.
Numerous genes encoding BMPs, BMP receptors, and their downstream signal
transducers have been mutated in the mouse through targeted mutagenesis. This
review focuses on wha...
Efficient, inducible Cre‐recombinase activation in vascular endothelium
Tập 46 Số 2 - Trang 74-80 - 2008
AbstractIn recent years, gene‐targeting studies in mice have elucidated many
molecular mechanisms in vascular biology. However, it has been difficult to
apply this approach to the study of postnatal animals because mutations
affecting the vasculature are often embryonically lethal. We have therefore
generated transgenic mice that express a tamoxifen‐inducible form of Cre
recombinase (iCreERT2) in ...
A CamKIIα iCre BAC allows brain‐specific gene inactivation
Tập 31 Số 1 - Trang 37-42 - 2001
AbstractSummary: We describe the generation of transgenic mouse lines expressing
the Cre recombinase enzyme in brain under control of the CamKIIα gene present in
a BAC expression vector. The CamKIIα BAC transgene gave a faithful expression
pattern resembling the pattern of the endogenous CamKIIα gene. Specifically,
high levels of CamKIIα Cre were detected in hippocampus, cortex, and amygdala,
and ...
Generation of Pax2‐Cre mice by modification of a Pax2 bacterial artificial chromosome
Tập 38 Số 4 - Trang 195-199 - 2004
AbstractSummary: The Pax2 gene is expressed in the developing otocyst, kidney,
and midbrain–hindbrain boundary. We generated Pax2‐Cre transgenic lines by
modification of a Pax2 bacterial artificial chromosome (BAC). In one Pax2‐Cre
line, Cre mRNA starts to be expressed in the otic placode at the late presomite
stage. R26R reporter mouse analysis revealed that the Cre expression is
sufficient to de...
Characterization of Notch3‐deficient mice: Normal embryonic development and absence of genetic interactions with a Notch1 mutation
Tập 37 Số 3 - Trang 139-143 - 2003
AbstractThe Notch signaling pathway is an evolutionarily conserved signaling
mechanism and mutations in its components disrupt cell fate specification and
embryonic development in many organisms. To analyze the in vivo role of the
Notch3 gene in mice, we created a deletion allele by gene targeting. Embryos
homozygous for this mutation developed normally and homozygous mutant adults
were viable and...