hGFAP‐cre transgenic mice for manipulation of glial and neuronal function in vivo

Genesis - Tập 31 Số 2 - Trang 85-94 - 2001
Lang Zhuo1, Martin Theis2, Ikuri Álvarez-Maya3, Michael Brenner3, Klaus Willecke2, Albee Messing4
1Department of Pathobiological Sciences, Waisman Center, University of Wisconsin, 1500 Highland Avenue, Madison, WI 53705-2280, USA.
2Institut fur Genetik, Abt. Molekulargenetik, Universitat Bonn, Bonn, Germany
3Department of Neurobiology and Department of Physical Medicine and Rehabilitation, University of Alabama-Birmingham, Birmingham, Alabama
4Department of Pathobiological Sciences, Waisman Center and School of Veterinary Medicine; University of Wisconsin, Madison, Wisconsin

Tóm tắt

Abstract

With the goal of performing astrocyte‐specific modification of genes in the mouse, we have generated a transgenic line expressing Cre recombinase under the control of the human glial fibrillary acidic protein (hGFAP) promoter. Activity was monitored by crossing the hGFAP‐cre transgenics with either of two reporter lines carrying a lacZ gene whose expression requires excision of loxP‐flanked stop sequences. We found that lacZ expression was primarily limited to the central nervous system, but therein was widespread in neurons and ependyma. Cell types within the brain that notably failed to activate lacZ expression included Purkinje neurons of the cerebellum and choroid plexus epithelium. Onset of Cre expression began in the forebrain by e13.5, suggesting that the hGFAP promoter is active in a multi‐potential neural stem cell. genesis 31:85–94, 2001. © 2001 Wiley‐Liss, Inc.

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Genesis

Tập 31 Số 2

85-94

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