Current Protocols in Molecular Biology
SCOPUS (2001-2020)
1934-3639
1934-3647
Mỹ
Cơ quản chủ quản: John Wiley & Sons Inc.
Lĩnh vực:
Medicine (miscellaneous)Molecular Biology
Các bài báo tiêu biểu
ATAC‐seq: A Method for Assaying Chromatin Accessibility Genome‐Wide
Tập 109 Số 1 - 2015
AbstractThis unit describes Assay for Transposase‐Accessible Chromatin with
high‐throughput sequencing (ATAC‐seq), a method for mapping chromatin
accessibility genome‐wide. This method probes DNA accessibility with hyperactive
Tn5 transposase, which inserts sequencing adapters into accessible regions of
chromatin. Sequencing reads can then be used to infer regions of increased
accessibility, as we...
Galaxy: A Web‐Based Genome Analysis Tool for Experimentalists
Tập 89 Số 1 - 2010
AbstractHigh‐throughput data production has revolutionized molecular biology.
However, massive increases in data generation capacity require analysis
approaches that are more sophisticated, and often very computationally
intensive. Thus, making sense of high‐throughput data requires informatics
support. Galaxy (http://galaxyproject.org) is a software system that provides
this support through a fra...
Metabolomics by Gas Chromatography–Mass Spectrometry: Combined Targeted and Untargeted Profiling
Tập 114 Số 1 - 2016
AbstractGas chromatography–mass spectrometry (GC‐MS)–based metabolomics is ideal
for identifying and quantitating small‐molecule metabolites (<650 Da), including
small acids, alcohols, hydroxyl acids, amino acids, sugars, fatty acids,
sterols, catecholamines, drugs, and toxins, often using chemical derivatization
to make these compounds sufficiently volatile for gas chromatography. This unit
shows...
Introduction of DNA into Yeast Cells
Tập 27 Số 1 - 1994
AbstractThe most commonly used yeast transformation protocol is the lithium
acetate procedure (described here). It is reasonably fast and provides a
transformation efficiency of 105 to 106 transformants/μg. This efficiency rivals
that achieved for most, but not all, strains with the more difficult and
time‐consuming spheroplast procedure presented here. However, the fastest and
easiest of the tran...
Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination
Tập 78 Số 1 - 2007
AbstractThe bacterial chromosome and plasmids can be engineered in vivo by
homologous recombination using PCR products and synthetic oligonucleotides as
substrates. This is possible because bacteriophage‐encoded recombination
functions efficiently to recombine sequences with homologies as short as 35 to
40 bases. This recombineering allows DNA sequences to be inserted or deleted
without regard to ...
Mapping Regulatory Factors by Immunoprecipitation from Native Chromatin
Tập 110 Số 1 - 2015
AbstractOccupied Regions of Genomes from Affinity‐purified Naturally Isolated
Chromatin (ORGANIC) is a high‐resolution method that can be used to
quantitatively map protein‐DNA interactions with high specificity and
sensitivity. This method uses micrococcal nuclease (MNase) digestion of
chromatin and low‐salt solubilization to preserve protein‐DNA complexes,
followed by immunoprecipitation and pai...
Using Barcoded HIV Ensembles (B‐HIVE) for Single Provirus Transcriptomics
Tập 122 Số 1 - 2018
AbstractThe latent HIV reservoir is the main barrier to curing AIDS, because
infected cells escape the immune system and antiretroviral therapies. Developing
new treatment strategies requires technologies to trace latent proviruses. Here,
we describe a genome‐wide technique called Barcoded HIV Ensembles (B‐HIVE) to
measure HIV expression at the single provirus level. The principle of B‐HIVE is
to ...
Acid Hydrolysis for Release of Monosaccharides
Tập 32 Số 1 - 1995
AbstractThe first step in obtaining a compositional analysis of a glycoconjugate
is the release of the individual monosaccharide constituents. Mild acid
hydrolysis to release fucosyl residues from glycoconjugates is described in this
unit, in addition to mild acid hydrolysis to release sialic acids from
glycoconjugates, along with dialysis and column chromatography procedures to
purify the sialic ...