Acid Hydrolysis for Release of Monosaccharides

Current Protocols in Molecular Biology - Tập 32 Số 1 - 1995
Adriana E. Manzi1
1University of California, San Diego, La Jolla, California

Tóm tắt

AbstractThe first step in obtaining a compositional analysis of a glycoconjugate is the release of the individual monosaccharide constituents. Mild acid hydrolysis to release fucosyl residues from glycoconjugates is described in this unit, in addition to mild acid hydrolysis to release sialic acids from glycoconjugates, along with dialysis and column chromatography procedures to purify the sialic acids. Strong acid hydrolysis to release all monosaccharides from glycoconjugates is also detailed.

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Current Protocols in Molecular Biology

Tập 32 Số 1

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