Cell Biology International
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Tissue‐specific expression of dentin sialophosphoprotein (DSPP) and its polymorphisms in mouse tissues
Cell Biology International - Tập 33 - Trang 816-829 - 2009
AbstractDentin sialophosphoprotein (DSPP) consists of dentin sialoprotein (DSP)
and dentin phosphoprotein (DPP). DSPP is highly expressed in mineralized
tissues. However, recent studies have shown that DSPP is also expressed in
several active metabolic ductal epithelial tissues and exists in a variety of
sequences. We have investigated DSPP expression in various mouse tissues using
RT‐PCR, in situ...
Realgar‐induced differentiation is associated with MAPK pathways in HL‐60 cells
Cell Biology International - Tập 32 - Trang 1497-1505 - 2008
AbstractThe clinical efficacy and safety of realgar (arsenic sulfide, As4S4) in
the treatment of acute promyelocytic leukemia in China have given rise to an
upsurge in research on the underlying mechanism. We prepared realgar
nanoparticles (RNPs) to examine their effect on the differentiation of HL‐60
cells. Treatment with RNPs at 6 μM for 72 h induced cell differentiation that
was assessed by mor...
ROTOKINESIS, A NOVEL PHENOMENON OF CELL LOCOMOTION‐ASSISTED CYTOKINESIS IN THE CILIATE TETRAHYMENA THERMOPHILA
Cell Biology International - Tập 23 - Trang 841-848 - 1999
AbstractThe mechanism responsible for final cell separation at the end of
cytokinesis is currently unknown. Knockout strains of the ciliate, Tetrahymena
thermophila lacking the kinesin‐II homologous molecular motors, Kin1p and Kin2p
are paralyzed due to their complete loss of cilia and undergo frequent
cytokinesis failures. Observations of live dividing cells revealed that cleavage
furrow ingressi...
THE EFFECT OF CYTOCHALASIN J ON KINETOCHORE STRUCTURE IN PTK1CELLS IS MITOTIC CYCLE DEPENDENT
Cell Biology International - Tập 25 - Trang 815-820 - 2001
Mitotic PtK1cells were arrested in mitosis with nocodazole to determine the
effect of cytochalasin J (CJ) on kinetochore structure in arrested and
nocodazole‐released cells. In previous studies it was shown that CJ had a more
pronounced effect on alteration of kinetochore structure and spindle microtubule
(MT) architecture when applied during prophase or prometaphase. In this study,
mitotic cells ...
An in vitro model to evaluate cell adhesion to metals used in implantation shows significant differences between palladium and gold or platinum
Cell Biology International - Tập 28 - Trang 541-547 - 2004
AbstractAdhesion of tissue cells to metallic implants is a major factor that is
important for proper tissue integration. Adhesion of Swiss mouse 3T3 fibroblasts
to gold, platinum and palladium surfaces was investigated. Immunofluorescence
staining for the integrin subunits αv and β1 and the focal contact protein
vinculin revealed that cells growing on gold and platinum expressed many focal
contact...
Fifth dimension of life and the 4/5 allometric scaling law for human brain
Cell Biology International - Tập 28 - Trang 809-815 - 2004
AbstractBrain cells are not spherical. The basal metabolic rate (B) of a
spherical cell scales as B ∼ r2, where r is the radius of the cell; that of a
brain cell scales as B ∼ rd, where r is the characteristic radius of the cell
and d is the fractal dimensionality of its contour. The fractal geometry of the
cell leads to a 4/5 allometric scaling law for human brain, uniquely endowing
humans with a...
Changes in signaling pathways of cell proliferation and apoptosis during NK/Ly lymphoma aging
Cell Biology International - Tập 32 - Trang 1057-1063 - 2008
AbstractExpression of specific proteins involved in regulation of cell
proliferation and apoptosis was studied at the initial (7–8 days after tumor
inoculation), median (13–14 days), and terminal (20–21 days) stages of murine
NK/Ly lymphoma development. Western‐blot analysis using antibodies to MEK—ERK
signaling pathway, E2F‐1/2 and c‐Myc, pSTAT1, pSTAT3, pSTAT5, anti‐apoptotic
Bcl‐XL and pro‐apop...
OESTRADIOL REGULATED PROGRAMMED CELL DEATH IN RAT VAGINA: TERMINAL DIFFERENTIATION OR APOPTOSIS?
Cell Biology International - Tập 22 - Trang 105-113 - 1998
Rat vaginal epithelial cells (VEC) undergo division and differentiation under
the influence of oestradiol in a programmed manner. The differentiation process
of VEC leads to keratinization, cornification and subsequent desquamation of the
dead cells. This process of programmed cell death, referred to as terminal
differentiation may share some common pathways with cell death by apoptosis but
differ...
Three‐dimensional matrix induces sustained activation of ERK1/2 via Src/Ras/Raf signaling pathway
Cell Biology International - Tập 32 - Trang 229-234 - 2008
AbstractResearch in cell signaling often depends on tissue culture, but the
artificial substrates used to grow cells in vitro are likely to distort the
conclusions, particularly when adhesion‐mediated signaling events are
investigated. Studies of signal transduction pathways operating in cells grown
in three‐dimensional (3D) matrices provide a better system, giving a closer
insight of the cell sig...
Transglutaminase 2 activity promotes membrane resealing after mechanical damage in the lung cancer cell line A549
Cell Biology International - Tập 32 - Trang 928-934 - 2008
AbstractTransglutaminase is a Ca2+‐dependent enzyme catalyzing protein
cross‐linking reactions. We investigated the contribution of this enzyme to the
resealing of the injured plasma membrane in animal cells, using a lung
cancer‐derived cell line, A549. After mechanical injury by razor‐scratching, the
level of membrane resealing was estimated by differential incorporation of
dextrans labeled with ...
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