Induction of apoptosis by Hax‐1 siRNA in melanoma cells

Cell Biology International - Tập 33 - Trang 548-554 - 2009
Wen-Bin Li1, Jie Feng1, Song-Mei Geng1, Peng-Yu Zhang2, Xiao-Ning Yan1, Gang Hu1, Cai-Qing Zhang1, Bing-Jun Shi1
1Department of Dermatology, The Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, The West Five Road, 157#, Xi'an, Shaanxi 710004, PR China
2Department of Clinical Hematology, The Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710004, PR China

Tóm tắt

AbstractHS1‐associated protein X‐1 (Hax‐1) is a novel intracellular protein and recent studies suggested that it is an anti‐apoptotic factor in different tumors. Hax‐1 expression was upregulated in various metastatic tumors and cancer cell lines, including melanoma. To understand the role of Hax‐1 in melanoma development and progression, we constructed Hax‐1 short interfering RNA (siRNA) expression vectors to downregulate Hax‐1 expression in a human melanoma A375 cell line. One of the two Hax‐1 RNA interference (RNAi) constructs significantly reduced melanoma cell viability, which was due to induction of apoptosis in A375 cells. Molecularly, the induced apoptosis through downregulation of Hax‐1 expression was mediated by activation of caspase‐3 and poly‐ADP‐ribose polymerase (PARP) enzymatic activity in A375 cells. The data indicate that Hax‐1 plays a role in suppression of apoptosis and promotion of melanoma cell growth, suggesting that this Hax‐1 siRNA has a therapeutic indication in control of melanoma.

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Cell Biology International

Tập 33

548-554

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