Comparison between osteoblasts derived from human dental pulp stem cells and osteosarcoma cell lines

Cell Biology International - Tập 32 - Trang 733-738 - 2008
Annalisa Palmieri1, Furio Pezzetti1, Antonio Graziano2, D'Aquino Riccardo2, Ilaria Zollino3, Giorgio Brunelli3, Marcella Martinelli1, Marzia Arlotti1, Francesco Carinci3
1Centre of Molecular Genetics, CARISBO Foundation, Institute of Histology and General Embryology, School of Medicine, University of Bologna, Bologna, Italy
2Dental Clinic, Second University of Naples, Naples, Italy
3Chair of Maxillofacial Surgery, School of Medicine, University of Ferrara, Arcispedale S. Anna, Corso Giovecca 203, 44100 Ferrara, Italy

Tóm tắt

AbstractStem cells derived from human dental pulp are able to differentiate into osteoblasts and are a potential source of autologous bone. The aim of this study was to compare genes differentially expressed in osteoblastoids from human dental pulp (OHDP) to osteosarcoma cells (OCs).Human dental pulp was extracted and immersed in a digestive solution. Cells were cultured and selected using c‐kit, CD34, CD45 and STRO‐1 antibodies. In parallel, two OCs (i.e., SAOS2 and TE85) were cultured. RNA was extracted from different populations of cells and cDNA was used for the hybridisation of human 19.2 K DNA microarrays.We identified several differences in gene expression between OHDP and OCs. Some down‐regulated OHDP genes, such as RUNX1, MAP4K4 and PRDM2, are involved in bone development, cell motility and transcript regulation. Gene expression in OHDP is significantly different from that in OCs, suggesting differences in cell function and activity between these cells.

Tài liệu tham khảo

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Cell Biology International

Tập 32

733-738

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