Hans V. Westerhoff, Mary H. O’Dea, Anthony Maxwell, Martin Gellert
Using purified DNA gyrase to supercoil circular plasmid pBR322 DNA, we examined
how the linking number attained at the steady state (‘static head’) varies with
the concentrations of ATP and ADP, both in the absence and presence of
spermidine. In the absence of spermidine at total adenine nucleotide
concentrations between 0.35 and 1.4 mM, the static-head linking number was
independent of the sum co... hiện toàn bộ