Bioresources and Bioprocessing
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A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis
Bioresources and Bioprocessing - Tập 9 - Trang 1-12 - 2022
To mimic the Escherichia coli T7 protein expression system, we developed a
facile T7 promoter-based protein expression system in an industrial
microorganism Bacillus subtilis. This system has two parts: a new B. subtilis
strain SCK22 and a plasmid pHT7. To construct strain SCK22, the T7 RNA
polymerase gene was inserted into the chromosome, and several genes, such as two
major protease genes, a spo...
Kinetic modeling and process analysis for photo-production of β-carotene in Dunaliella salina
Bioresources and Bioprocessing - Tập 9 - Trang 1-13 - 2022
Dunaliella salina is a green microalga with the great potential to generate
natural β-carotene. However, the corresponding mathematical models to guide
optimized production of β-carotene in Dunaliella salina (D. salina) are not yet
available. In this study, dynamic models were proposed to simulate effects of
environmental factors on cell growth and β-carotene production in D. salina
using online m...
Elucidating the degradation pattern of a new cold-tolerant pectate lyase used for efficient preparation of pectin oligosaccharides
Bioresources and Bioprocessing - - 2021
AbstractThe cold-active pectate lyases have drawn increasing attention in food
and biotechnological applications due to their ability to retain high catalytic
efficiency under lower temperatures, which could be helpful for energy saving,
cost reduction and flavor preservation. Herein, a new cold-tolerant pectate
lyase (ErPelPL1) gene from Echinicola rosea was cloned and heterologously
expressed in...
Cell-culture growth conditions resulting in the oxidation of a recombinant antigen-binding fragment
Bioresources and Bioprocessing - - 2019
Use of Quality-by-Design (QbD) tools is becoming an important part of the
bioprocessing industry when developing a process for manufacturing operations to
ensure the robustness and reproducibility of the biologic product. In the
present study, a QbD tool, Design of Experiments (DOE), was utilized to optimize
a bioprocess for the production of a CHO recombinant antigen-binding fragment
(rFab) in sm...
Parametric study of glycerol and contaminants removal from biodiesel through solvent-aided crystallization
Bioresources and Bioprocessing - Tập 8 - Trang 1-12 - 2021
At present, biodiesel is known as an alternative fuel globally. It is also known
that the purification of biodiesel before consumption is mandatory to comply
with international standards. Commonly, purification using water washing
generates a massive amount of wastewater with a high content of organic
compounds that can harm the environment. Therefore, this study applied and
tested a waterless met...
Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis
Bioresources and Bioprocessing - Tập 1 - Trang 1-10 - 2014
In recent years, green synthesis of silver nanoparticles (AgNPs) has gained much
interest from chemists and researchers. In this concern, Indian flora has yet to
divulge innumerable sources of cost-effective non-hazardous reducing and
stabilizing compounds utilized in preparing AgNPs. This study investigates an
efficient and sustainable route of AgNP preparation from 1 mM aqueous AgNO3
using leaf ...
In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2
Bioresources and Bioprocessing - Tập 7 Số 1 - 2020
AbstractThe present study evaluated the potential biological activities of
rhizospheric fungi isolated from the Achanakmar Biosphere Reserve, India.
Fungus, Talaromyces purpureogenus isolate-ABRF2 from the soil of the Achanakmar
biosphere was characterized by using morphological, biochemical and molecular
techniques. Fungus was screened for the production of secondary metabolites
using a specific ...
Supercritical fluid extraction of polyphenols from lees: overall extraction curve, kinetic data and composition of the extracts
Bioresources and Bioprocessing - Tập 2 - Trang 1-13 - 2015
The increasing incidence of degenerative diseases has attracted the interest in
the obtaining of bioactive compounds. Since seeds and skins from grapes are
important sources of polyphenols which have been associated with cancer
incidence decreasing, then, one of the pisco (alcoholic beverage made of grape)
manufacturing byproduct such as lees, could be a potential source of
polyphenols. Supercriti...
Enhancement of acetate production in hydrogen-mediated microbial electrosynthesis reactors by addition of silica nanoparticles
Bioresources and Bioprocessing - Tập 10 - Trang 1-10 - 2023
Microbial electrosynthesis (MES) is a promising technology for CO2 fixation and
electrical energy storage. Currently, the low current density of MES limits its
practical application. The H2-mediated and non-biofilm-driven MES could work
under higher current density, but it is difficult to achieve high coulombic
efficiency (CE) due to low H2 solubility and poor mass transfer. Here, we
proposed to e...
Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem
Bioresources and Bioprocessing - Tập 6 - Trang 1-11 - 2019
Haloacid dehalogenase (HAD)-like hydrolases represent the largest superfamily of
phosphatases, which release inorganic phosphate from phosphate containing
compounds, such as sugar phosphates. The HAD-like phosphatases with highly
substrate specificity, which perform irreversible dephosphorylation, are always
integrated into in vitro synthetic enzymatic biosystems as the last enzymatic
step for the...
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