Bioresources and Bioprocessing

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A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis
Bioresources and Bioprocessing - Tập 9 - Trang 1-12 - 2022
Jing Ye, Yunjie Li, Yuqing Bai, Ting Zhang, Wei Jiang, Ting Shi, Zijian Wu, Yi-Heng P. Job Zhang
To mimic the Escherichia coli T7 protein expression system, we developed a facile T7 promoter-based protein expression system in an industrial microorganism Bacillus subtilis. This system has two parts: a new B. subtilis strain SCK22 and a plasmid pHT7. To construct strain SCK22, the T7 RNA polymerase gene was inserted into the chromosome, and several genes, such as two major protease genes, a spo...... hiện toàn bộ
Kinetic modeling and process analysis for photo-production of β-carotene in Dunaliella salina
Bioresources and Bioprocessing - Tập 9 - Trang 1-13 - 2022
Yimei Xi, Jiali Zhang, Fantao Kong, Jian Che, Zhanyou Chi
Dunaliella salina is a green microalga with the great potential to generate natural β-carotene. However, the corresponding mathematical models to guide optimized production of β-carotene in Dunaliella salina (D. salina) are not yet available. In this study, dynamic models were proposed to simulate effects of environmental factors on cell growth and β-carotene production in D. salina using online m...... hiện toàn bộ
Elucidating the degradation pattern of a new cold-tolerant pectate lyase used for efficient preparation of pectin oligosaccharides
Bioresources and Bioprocessing - - 2021
Li Zheng, Zilong Guo, Shengsheng Cao, Benwei Zhu
AbstractThe cold-active pectate lyases have drawn increasing attention in food and biotechnological applications due to their ability to retain high catalytic efficiency under lower temperatures, which could be helpful for energy saving, cost reduction and flavor preservation. Herein, a new cold-tolerant pectate lyase (ErPelPL1) gene from Echinicola ro...... hiện toàn bộ
Cell-culture growth conditions resulting in the oxidation of a recombinant antigen-binding fragment
Bioresources and Bioprocessing - - 2019
Khandaker Siddiquee, Cheng Zhao, Michelle A. Stemler, Bill Zeck, Jeffrey R. Fishpaugh, Steven P. Allen
Use of Quality-by-Design (QbD) tools is becoming an important part of the bioprocessing industry when developing a process for manufacturing operations to ensure the robustness and reproducibility of the biologic product. In the present study, a QbD tool, Design of Experiments (DOE), was utilized to optimize a bioprocess for the production of a CHO recombinant antigen-binding fragment (rFab) in sm...... hiện toàn bộ
Parametric study of glycerol and contaminants removal from biodiesel through solvent-aided crystallization
Bioresources and Bioprocessing - Tập 8 - Trang 1-12 - 2021
Mohd. Afnan Ahmad, Arun Letchumanan, Shafirah Samsuri, Wan Nur Athirah Mazli, Juniza Md Saad
At present, biodiesel is known as an alternative fuel globally. It is also known that the purification of biodiesel before consumption is mandatory to comply with international standards. Commonly, purification using water washing generates a massive amount of wastewater with a high content of organic compounds that can harm the environment. Therefore, this study applied and tested a waterless met...... hiện toàn bộ
Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis
Bioresources and Bioprocessing - Tập 1 - Trang 1-10 - 2014
Priya Banerjee, Mantosh Satapathy, Aniruddha Mukhopahayay, Papita Das
In recent years, green synthesis of silver nanoparticles (AgNPs) has gained much interest from chemists and researchers. In this concern, Indian flora has yet to divulge innumerable sources of cost-effective non-hazardous reducing and stabilizing compounds utilized in preparing AgNPs. This study investigates an efficient and sustainable route of AgNP preparation from 1 mM aqueous AgNO3 using leaf ...... hiện toàn bộ
In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2
Bioresources and Bioprocessing - Tập 7 Số 1 - 2020
Mahendra Kumar Sahu, Komal Kaushik, Amitava Das, Harit Jha
AbstractThe present study evaluated the potential biological activities of rhizospheric fungi isolated from the Achanakmar Biosphere Reserve, India. Fungus, Talaromyces purpureogenus isolate-ABRF2 from the soil of the Achanakmar biosphere was characterized by using morphological, biochemical and molecular techni...... hiện toàn bộ
Supercritical fluid extraction of polyphenols from lees: overall extraction curve, kinetic data and composition of the extracts
Bioresources and Bioprocessing - Tập 2 - Trang 1-13 - 2015
Angela M. Farías-Campomanes, Mauricio A. Rostagno, Joel J. Coaquira-Quispe, M. Angela A. Meireles
The increasing incidence of degenerative diseases has attracted the interest in the obtaining of bioactive compounds. Since seeds and skins from grapes are important sources of polyphenols which have been associated with cancer incidence decreasing, then, one of the pisco (alcoholic beverage made of grape) manufacturing byproduct such as lees, could be a potential source of polyphenols. Supercriti...... hiện toàn bộ
Enhancement of acetate production in hydrogen-mediated microbial electrosynthesis reactors by addition of silica nanoparticles
Bioresources and Bioprocessing - Tập 10 - Trang 1-10 - 2023
Zeyan Pan, Zhuangzhuang Liu, Xiaona Hu, Kai Cui, Wenfang Cai, Kun Guo
Microbial electrosynthesis (MES) is a promising technology for CO2 fixation and electrical energy storage. Currently, the low current density of MES limits its practical application. The H2-mediated and non-biofilm-driven MES could work under higher current density, but it is difficult to achieve high coulombic efficiency (CE) due to low H2 solubility and poor mass transfer. Here, we proposed to e...... hiện toàn bộ
Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem
Bioresources and Bioprocessing - Tập 6 - Trang 1-11 - 2019
Wei Wang, Dongdong Meng, Qiangzi Li, Zhimin Li, Chun You
Haloacid dehalogenase (HAD)-like hydrolases represent the largest superfamily of phosphatases, which release inorganic phosphate from phosphate containing compounds, such as sugar phosphates. The HAD-like phosphatases with highly substrate specificity, which perform irreversible dephosphorylation, are always integrated into in vitro synthetic enzymatic biosystems as the last enzymatic step for the...... hiện toàn bộ
Tổng số: 519   
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