BioChip Journal
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Study on teratogenic effects of methotrexate; Alteration of gene expression pattern in human placenta choriocarcinoma (JEG-3) cells
BioChip Journal - Tập 4 - Trang 189-196 - 2010
Methotrexate is a well known chemotherapeutic agent, extensively used for leukemia and other cancers. Although its efficacy is wide, methotrexate can cause serious or life-threatening toxicities on liver, lungs, kidney, and immune system. Teratogenicity is one of the methotrexate-induced side effects. So, we performed with JEG-3, human placenta choriocarcinoma cell line, to identify the differenti......
Orientation Control of the Molecular Recognition Layer for Improved Sensitivity: a Review
BioChip Journal - Tập 13 - Trang 82-94 - 2019
Biosensors have been used in various fields of biological analysis, such as for quantification of analytes and the study of molecule-molecule interactions. Orientation control of the molecular recognition layer is one of the easiest and most effective ways to improve the sensitivity of biosensors. In this review, the orientation control of molecular recognition molecules, such as antibodies, aptam......
Thermoplasmonic Scaffold Design for the Modulation of Neural Activity in Three-Dimensional Neuronal Cultures
BioChip Journal - Tập 16 - Trang 451-462 - 2022
Neuromodulation has made great strides in recent years, but in vitro studies have been limited to two-dimensional cell cultures, far from in vivo conditions. In this study, we realized a novel thermoplasmonic platform for modulating the neural activity of three-dimensional cell cultures, providing a new tool to bring in vitro neuromodulation studies into a three-dimensional environment. The photos......
Cytotoxic effects of surface-modified quantum dots on neuron-like PC12 cells cultured inside microfluidic devices
BioChip Journal - Tập 4 - Trang 82-88 - 2010
This paper describes the cytotoxic effects of surface-modified quantum dots (QDs) on neuronlike PC12 cells under localized exposure conditions. PC12 cells were cultured and differentiated on a two-compartment microfluidic neuronal device with the aim of preferentially isolating axons from their cell bodies and thus providing localized exposure of cell bodies or axons to QDs. In addition, we used t......
Laser-induced heating for cell release and cellular DNA denaturation in a microfluidics
BioChip Journal - Tập 7 - Trang 319-324 - 2013
In this study, a microfluidics is integrated with a laser to develop a microfluidic system for single-cell capture and cellular DNA denaturation. The results show that the cells encountered low hydrodynamic stress at low velocities and, thus, could be captured by a cell capture structure. Laser heating of the solution at low velocity produced bubbles that pushed the cells away from the capturing a......
ProteoChip-based screening system of novel inhibitors against the interaction of VEGF165 and β-Amyloid1–42
BioChip Journal - Tập 5 - Trang 299-303 - 2011
Vascular endothelial growth factor (VEGF) protects neuronal cells from amyloid-β(Aβ)-induced neurotoxicity in the brain of Alzheimer’s disease (AD) patient. However, the accumulation of VEGF in amyloid plaques may induce local VEGF deficiency and promote development of the disease. Here we investigated that Aβ1–42 can interact with heparin binding domain (HBD) of VEGF using ProteoChip technology. ......
MicroRNA microarray analysis of human umbilical vein endothelial cells exposed to benzo(a)pyrene
BioChip Journal - Tập 6 - Trang 191-196 - 2012
MicroRNAs (miRNAs) are small non-coding RNA molecules that function as potent negative regulators of gene expression. They play a critical role in the regulation of genes involved in the cellular and molecular responses to toxicants. Benzo(a)pyrene (BaP) is an environmental contaminant found in cigarette smoke and is one of the polycyclic aromatic hydrocarbons (PAHs). BaP is considered a major con......
Fluorescence immunochip assay for thyroid stimulating hormone in whole blood
BioChip Journal - Tập 7 - Trang 408-414 - 2013
The determination of blood level of thyroid stimulating hormone (TSH) is an important biomarker for the clinical assessment of thyroid status. Here, we presented a new fluorescence (FL) immunochip TSH assay system, which was developed with a platform of point-of-care test (POCT) for clinical applications. The assay system adopted a lateral-flow immunochromatographic technology and consisted of ant......
Multiplex detection of pathogens using an immunochromatographic assay strip
BioChip Journal - Tập 4 - Trang 305-312 - 2010
An enzyme-linked immunosorbent assay (ELISA) was performed on an immunochromatographic strip for the multiplex detection of Salmonella typhimurium, Staphylococcus aureus, Legionella pneumophila, and Escherichia coli O157:H7. The immunostrip assay was completed within 20 min. An optical (colourimetric) signal could be successfully generated using the proposed immunostrip system to selectively detec......
Genome-wide gene expression analysis of Patrinia scabiosaefolia reveals an antibiotic effect
BioChip Journal - Tập 5 - Trang 246-254 - 2011
Patrinia scabiosaefolia (PS) clinically had been prescribed in oriental ethnopharmacology, but its exact mechanism is unknown. In this study, we analyzed molecular perspective of antibiotic effects of PS. Escherichia coli O157:H7 was changed in four main mechanisms; (1) Cell wall biosynthesis decreased, (2) DNA transcription consists of folic acid metabolism interruption, ......
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