American Society for Microbiology
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Oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16S-23S rRNA internal transcribed spacersUniversal primers targeting conserved sequences flanking the 3' end of the 16S and the 5' end of the 23S rRNA genes (rDNAs) were used to amplify the 16S-23S rDNA internal transcribed spacers (ITS) from eight species of pseudomonads which have been associated with human infections. Amplicons from reference strains of Pseudomonas aeruginosa, Pseudomonas cepacia, Pseudomonas gladioli, Pseudom... ... hiện toàn bộ
American Society for Microbiology - Tập 2 Số 4 - Trang 448-453 - 1995
Will immunogenicity limit the use, efficacy, and future development of therapeutic monoclonal antibodies?While monoclonal antibodies show promise for use in the treatment of a variety of disease states, including cancer, autoimmune disease, and allograft rejection, generation of anti-antibody responses still remains a problem. For example, 50% of the patients who receive OKT3 produce blocking antibodies that interfere with its binding to T cells, thus decreasing the therapeutic effect (51). H... ... hiện toàn bộ
American Society for Microbiology - Tập 1 Số 4 - Trang 365-372 - 1994
Identification of Potentially DiagnosticLeishmania braziliensisAntigens in Human Cutaneous Leishmaniasis by Immunoblot AnalysisABSTRACT The antibody response in patients with American cutaneous leishmaniasis was analyzed by immunoblotting with soluble and insoluble antigens ofLeishmania braziliensis . The recognition of the 27- and/or 30-kDa soluble antigens was considered relevant for the diagnosis of cutaneous leishmaniasis. Immunoblotting was found to be signifi... ... hiện toàn bộ
American Society for Microbiology - Tập 7 Số 2 - Trang 318-321 - 2000
Long-Term Persistence of Immunoglobulin A (IgA) and IgM Antibodies against Human Cytomegalovirus in Solid-Organ Transplant RecipientsABSTRACT
The retrospective analysis of 494 solid-organ transplant recipients revealed that during the follow-up period (mean duration, 3.2 years) 184 (88%) of 209 anti-human cytomegalovirus (HCMV) immunoglobulin A (IgA)-positive patients remained IgA positive, as did 128 (74.85%) of 171 anti-HCMV IgM-positive patients. We conclude that anti-HCMV IgA and I... ... hiện toàn bộ
American Society for Microbiology - Tập 6 Số 4 - Trang 621-623 - 1999
Antibody Responses toAcinetobacterspp. andPseudomonas aeruginosain Multiple Sclerosis: Prospects for Diagnosis Using the Myelin-Acinetobacter-Neurofilament Antibody IndexABSTRACT Antibody responses toAcinetobacter (five strains),Pseudomonas aeruginosa ,Escherichia coli , myelin basic protein (MBP), and neurofilaments were measured in sera from 26 multiple sclerosis (MS) patients, 20 patients with cerebrovascular accidents (CVA), 10 patients with viral ence... ... hiện toàn bộ
American Society for Microbiology - Tập 8 Số 6 - Trang 1181-1188 - 2001
Comparison of Neutralizing and Hemagglutination-Inhibiting Antibody Responses to Influenza A Virus Vaccination of Human Immunodeficiency Virus-Infected IndividualsABSTRACT
A neutralization enzyme immunoassay (N-EIA) was used to determine the neutralizing serum antibody titers to influenza A/Taiwan/1/86 (H1N1) and Beijing/353/89 (H3N2) viruses after vaccination of 51 human immunodeficiency virus (HIV) type 1-infected individuals and 10 healthy noninfected controls against influenza virus infection. Over... ... hiện toàn bộ
American Society for Microbiology - Tập 5 Số 1 - Trang 114-117 - 1998
In Vitro p24 Antigen-Stimulated Lymphocyte Proliferation and β-Chemokine Production in Human Immunodeficiency Virus Type 1 (HIV-1)-Seropositive Subjects after Immunization with an Inactivated gp120-Depleted HIV-1 Immunogen (Remune)ABSTRACT We examined the effect of immune stimulation by a human immunodeficiency virus type 1 (HIV-1) immunogen (Remune) compared to a non-HIV vaccine (influenza) on HIV-1-specific immune responses in HIV-1-seropositive subjects. HIV-1 p24 antigen-stimulated lymphocyte proliferation was not augmented after immunization with the influenza vaccine. In contrast, subje... ... hiện toàn bộ
American Society for Microbiology - Tập 5 Số 3 - Trang 308-312 - 1998
Helicobacter pylori Intrafamilial Infections: Change in Source of Infection of a Child from Father to Mother after Eradication TherapyABSTRACT
Biopsy specimens of the antrum and corpus were obtained from four
Helicobacter pylori
-infected members of a family and from the same boy (son 1) in whom the infection reappeared after simultaneous successful eradication treatment of three family members, excluding the mother. A tota... ... hiện toàn bộ
American Society for Microbiology - Tập 8 Số 4 - Trang 731-739 - 2001
A Flow Cytometric Opsonophagocytic Assay for Measurement of Functional Antibodies Elicited after Vaccination with the 23-Valent Pneumococcal Polysaccharide VaccineABSTRACT Opsonophagocytosis is the primary mechanism for clearance of pneumococci from the host, and the measurement of opsonophagocytic antibodies appears to correlate with vaccine-induced protection. We developed a semiautomated flow cytometric opsonophagocytosis assay using HL-60 granulocytes as effector cells and nonviable 5,6-carboxyfluorescein, succinimidyl es... ... hiện toàn bộ
American Society for Microbiology - Tập 6 Số 4 - Trang 581-586 - 1999
Utility of flow cytometric detection of CD69 expression as a rapid method for determining poly- and oligoclonal lymphocyte activationCD69 is a lymphoid activation antigen whose rapid expression (< or = 2 h postactivation) makes it amenable for the early detection of T-cell activation and for subset activation analyses. In the present study we evaluated the utility of flow cytometric detection of CD69 expression by T cells activated with polyclonal stimuli (anti-CD3 and staphylococcal enterotoxin B [SEB]) and oligoclo... ... hiện toàn bộ
American Society for Microbiology - Tập 3 Số 3 - Trang 301-304 - 1996
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