American Society for Microbiology
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Calcein-Acetyoxymethyl Cytotoxicity Assay: Standardization of a Method Allowing Additional Analyses on Recovered Effector Cells and Supernatants
American Society for Microbiology - Tập 8 Số 6 - Trang 1131-1135 - 2001
ABSTRACTCytotoxicity assays provide an in vitro evaluation of the lytic activity
of NK and T cells against tumors or transformed cells. However, none of these
methods allow the recovery of cells or supernatants after the assay. We
standardized a microcytotoxicity test using calcein-acetoxymethyl (calcein-AM)
dye that requires very small quantities of cells while maintaining the same
sensitivity as...
Diagnosis of Rickettsial Diseases Using Samples Dried on Blotting Paper
American Society for Microbiology - Tập 6 Số 4 - Trang 483-488 - 1999
ABSTRACTThe use of filter paper is an inexpensive and convenient method for
collecting, storing, and transporting blood samples for serological studies. In
addition, samples occupy little space and can be readily transported without
refrigeration. Rickettsial diseases often evolve according to an epidemic mode
and are now considered reemerging diseases, especially in developing countries,
under co...
A New Method with General Diagnostic Utility for the Calculation of Immunoglobulin G Avidity
American Society for Microbiology - Tập 6 Số 5 - Trang 725-728 - 1999
ABSTRACT The reference method for immunoglobulin G (IgG) avidity determination
includes reagent-consuming serum titration. Aiming at better IgG avidity
diagnostics, we applied a logistic model for the reproduction of antibody
titration curves. This method was tested with well-characterized serum panels
for cytomegalovirus, Epstein-Barr virus, rubella virus, parvovirus B19, and
Toxoplasma gondii . ...
Quantitative Fluorogenic PCR Assay for Measuring Ovine Herpesvirus 2 Replication in Sheep
American Society for Microbiology - Tập 8 Số 1 - Trang 123-128 - 2001
ABSTRACT A fluorogenic PCR specific for ovine herpesvirus 2 (OvHV-2) DNA was
developed and compared to a previously established conventional seminested PCR.
Testing of a total of 152 blood samples from both positive and negative animals
revealed that the results of both assays corresponded to each other in 100% of
the cases. A second fluorogenic PCR for genomic sheep DNA was required to
normalize ...
Helicobacter pylori Intrafamilial Infections: Change in Source of Infection of a Child from Father to Mother after Eradication Therapy
American Society for Microbiology - Tập 8 Số 4 - Trang 731-739 - 2001
ABSTRACT Biopsy specimens of the antrum and corpus were obtained from four
Helicobacter pylori -infected members of a family and from the same boy (son 1)
in whom the infection reappeared after simultaneous successful eradication
treatment of three family members, excluding the mother. A total of 18 to 60 H.
pylori isolates were obtained from each specimen and subjected to rRNA gene
restriction pa...
Will immunogenicity limit the use, efficacy, and future development of therapeutic monoclonal antibodies?
American Society for Microbiology - Tập 1 Số 4 - Trang 365-372 - 1994
While monoclonal antibodies show promise for use in the treatment of a variety
of disease states, including cancer, autoimmune disease, and allograft
rejection, generation of anti-antibody responses still remains a problem. For
example, 50% of the patients who receive OKT3 produce blocking antibodies that
interfere with its binding to T cells, thus decreasing the therapeutic effect
(51). HAMA resp...
Long-Term Persistence of Immunoglobulin A (IgA) and IgM Antibodies against Human Cytomegalovirus in Solid-Organ Transplant Recipients
American Society for Microbiology - Tập 6 Số 4 - Trang 621-623 - 1999
ABSTRACT The retrospective analysis of 494 solid-organ transplant recipients
revealed that during the follow-up period (mean duration, 3.2 years) 184 (88%)
of 209 anti-human cytomegalovirus (HCMV) immunoglobulin A (IgA)-positive
patients remained IgA positive, as did 128 (74.85%) of 171 anti-HCMV
IgM-positive patients. We conclude that anti-HCMV IgA and IgM testing for
management of clinically rel...
A Flow Cytometric Opsonophagocytic Assay for Measurement of Functional Antibodies Elicited after Vaccination with the 23-Valent Pneumococcal Polysaccharide Vaccine
American Society for Microbiology - Tập 6 Số 4 - Trang 581-586 - 1999
ABSTRACTOpsonophagocytosis is the primary mechanism for clearance of pneumococci
from the host, and the measurement of opsonophagocytic antibodies appears to
correlate with vaccine-induced protection. We developed a semiautomated flow
cytometric opsonophagocytosis assay using HL-60 granulocytes as effector cells
and nonviable 5,6-carboxyfluorescein, succinimidyl ester-labeledStreptococcus
pneumoni...
Antigenic Properties and Diagnostic Potential of Baculovirus-Expressed Infectious Bursal Disease Virus Proteins VPX and VP3
American Society for Microbiology - Tập 7 Số 4 - Trang 645-651 - 2000
ABSTRACT The routine technique for detecting antibodies specific to infectious
bursal disease virus (IBDV) is a serological evaluation by enzyme-linked
immunosorbent assay (ELISA) with preparations of whole virions as the antigens.
To avoid using complete virus in the standard technique, we have developed two
new antigens through the expression of the VPX and VP3 genes in insect cells.
VPX and esp...
Determination of CD4 and CD8 lymphocyte subsets by a new alternative fluorescence immunoassay
American Society for Microbiology - Tập 2 Số 3 - Trang 330-336 - 1995
The purpose of this study was to evaluate a new alternative fluorescence
immunoassay method (Zymmune CD4/CD8 Cell Monitoring Kit; Zynaxis, Inc., Malvern,
Pa.) for determining the absolute CD4+ and CD8+ T-lymphocyte concentrations in
whole blood. The investigation was performed as a two-site comparison of the
reference whole blood flow cytometric method with the Zymmune method. In this
investigatio...
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