American Society for Microbiology

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Calcein-Acetyoxymethyl Cytotoxicity Assay: Standardization of a Method Allowing Additional Analyses on Recovered Effector Cells and Supernatants
American Society for Microbiology - Tập 8 Số 6 - Trang 1131-1135 - 2001
Simona Neri, Erminia Mariani, Alessandra Meneghetti, Luca Cattini, Andrea Facchini
ABSTRACTCytotoxicity assays provide an in vitro evaluation of the lytic activity of NK and T cells against tumors or transformed cells. However, none of these methods allow the recovery of cells or supernatants after the assay. We standardized a microcytotoxicity test using calcein-acetoxymethyl (calcein-AM) dye that requires very small quantities of cells while ma...... hiện toàn bộ
Diagnosis of Rickettsial Diseases Using Samples Dried on Blotting Paper
American Society for Microbiology - Tập 6 Số 4 - Trang 483-488 - 1999
Didier Raoult
ABSTRACTThe use of filter paper is an inexpensive and convenient method for collecting, storing, and transporting blood samples for serological studies. In addition, samples occupy little space and can be readily transported without refrigeration. Rickettsial diseases often evolve according to an epidemic mode and are now considered reemerging diseases, especially ...... hiện toàn bộ
A New Method with General Diagnostic Utility for the Calculation of Immunoglobulin G Avidity
American Society for Microbiology - Tập 6 Số 5 - Trang 725-728 - 1999
Maria H. Korhonen, John Brunstein, Heikki Haario, Alexei Katnikov, R Rescaldani, Klaus Hedman
ABSTRACT The reference method for immunoglobulin G (IgG) avidity determination includes reagent-consuming serum titration. Aiming at better IgG avidity diagnostics, we applied a logistic model for the reproduction of antibody titration curves. This method was tested with well-characterized serum panels for cytomegalovirus, Epstein-Barr virus,...... hiện toàn bộ
Quantitative Fluorogenic PCR Assay for Measuring Ovine Herpesvirus 2 Replication in Sheep
American Society for Microbiology - Tập 8 Số 1 - Trang 123-128 - 2001
D. Hüssy, Norbert Stäuber, Christian M. Leutenegger, Stefan Rieder, Mathias Ackermann
ABSTRACT A fluorogenic PCR specific for ovine herpesvirus 2 (OvHV-2) DNA was developed and compared to a previously established conventional seminested PCR. Testing of a total of 152 blood samples from both positive and negative animals revealed that the results of both assays corresponded to each other in 100% of the cases. A second fluorogenic PCR for g...... hiện toàn bộ
Helicobacter pylori Intrafamilial Infections: Change in Source of Infection of a Child from Father to Mother after Eradication Therapy
American Society for Microbiology - Tập 8 Số 4 - Trang 731-739 - 2001
Ikue Taneike, Yukiko Tamura, Toshiaki Shimizu, Yuichiro Yamashiro, Tatsuo Yamamoto
ABSTRACT Biopsy specimens of the antrum and corpus were obtained from four Helicobacter pylori -infected members of a family and from the same boy (son 1) in whom the infection reappeared after simultaneous successful eradication treatment of three family members, excluding the mother. A tota...... hiện toàn bộ
Will immunogenicity limit the use, efficacy, and future development of therapeutic monoclonal antibodies?
American Society for Microbiology - Tập 1 Số 4 - Trang 365-372 - 1994
Kristine Kuus-Reichel, Lana S. Grauer, Linda M. Karavodin, Christine Knott, M Krusemeier, Neil E. Kay
While monoclonal antibodies show promise for use in the treatment of a variety of disease states, including cancer, autoimmune disease, and allograft rejection, generation of anti-antibody responses still remains a problem. For example, 50% of the patients who receive OKT3 produce blocking antibodies that interfere with its binding to T cells, thus decreasing the therapeutic effect (51). H...... hiện toàn bộ
Long-Term Persistence of Immunoglobulin A (IgA) and IgM Antibodies against Human Cytomegalovirus in Solid-Organ Transplant Recipients
American Society for Microbiology - Tập 6 Số 4 - Trang 621-623 - 1999
Bodo R. Eing, H. Baumeister, Joachim E. Kuehn, Guenter May
ABSTRACT The retrospective analysis of 494 solid-organ transplant recipients revealed that during the follow-up period (mean duration, 3.2 years) 184 (88%) of 209 anti-human cytomegalovirus (HCMV) immunoglobulin A (IgA)-positive patients remained IgA positive, as did 128 (74.85%) of 171 anti-HCMV IgM-positive patients. We conclude that anti-HCMV IgA and I...... hiện toàn bộ
A Flow Cytometric Opsonophagocytic Assay for Measurement of Functional Antibodies Elicited after Vaccination with the 23-Valent Pneumococcal Polysaccharide Vaccine
American Society for Microbiology - Tập 6 Số 4 - Trang 581-586 - 1999
Joseph Martinez, Sandra Romero‐Steiner, Tamara Pilishvili, Suzanne Barnard, Joseph Schinsky, David Goldblatt, George M. Carlone
ABSTRACTOpsonophagocytosis is the primary mechanism for clearance of pneumococci from the host, and the measurement of opsonophagocytic antibodies appears to correlate with vaccine-induced protection. We developed a semiautomated flow cytometric opsonophagocytosis assay using HL-60 granulocytes as effector cells and nonviable 5,6-carboxyfluorescein, succinimidyl es...... hiện toàn bộ
Antigenic Properties and Diagnostic Potential of Baculovirus-Expressed Infectious Bursal Disease Virus Proteins VPX and VP3
American Society for Microbiology - Tập 7 Số 4 - Trang 645-651 - 2000
Jorge L. Martı́nez-Torrecuadrada, Beatriz Lázaro, José F. Rodrígúez, J. Ignacio Casal
ABSTRACT The routine technique for detecting antibodies specific to infectious bursal disease virus (IBDV) is a serological evaluation by enzyme-linked immunosorbent assay (ELISA) with preparations of whole virions as the antigens. To avoid using complete virus in the standard technique, we have developed two new antigens through the expression of the VPX...... hiện toàn bộ
Determination of CD4 and CD8 lymphocyte subsets by a new alternative fluorescence immunoassay
American Society for Microbiology - Tập 2 Số 3 - Trang 330-336 - 1995
Thomas N. Denny, Bruce D. Jensen, E I Gavin, A G Louzao, Francis Vella, James M. Oleske, Wai-Man Wong
The purpose of this study was to evaluate a new alternative fluorescence immunoassay method (Zymmune CD4/CD8 Cell Monitoring Kit; Zynaxis, Inc., Malvern, Pa.) for determining the absolute CD4+ and CD8+ T-lymphocyte concentrations in whole blood. The investigation was performed as a two-site comparison of the reference whole blood flow cytometric method with the Zymmune method. In this inve...... hiện toàn bộ
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