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American Society for Microbiology

  1071-412X

  1098-6588

 

Cơ quản chủ quản:  N/A

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Các bài báo tiêu biểu

Inhibition of the Activities of Matrix Metalloproteinases 2, 8, and 9 by Chlorhexidine
Tập 6 Số 3 - Trang 437-439 - 1999
Renée Gendron, Daniel Grenier, Timo Sorsa, D. Mayrand
ABSTRACT Matrix metalloproteinases (MMPs) are a host cell-derived proteolytic enzyme family which plays a major role in tissue-destructive inflammatory diseases such as periodontitis. The aim of the present study was to evaluate the inhibitory effect of chlorhexidine (CHX) on MMP-2 (gelatinase A), MMP-9 (gelatinase B), and MMP-8 (collagenase ...... hiện toàn bộ
Calcein-Acetyoxymethyl Cytotoxicity Assay: Standardization of a Method Allowing Additional Analyses on Recovered Effector Cells and Supernatants
Tập 8 Số 6 - Trang 1131-1135 - 2001
Simona Neri, Erminia Mariani, Alessandra Meneghetti, Luca Cattini, Andrea Facchini
ABSTRACTCytotoxicity assays provide an in vitro evaluation of the lytic activity of NK and T cells against tumors or transformed cells. However, none of these methods allow the recovery of cells or supernatants after the assay. We standardized a microcytotoxicity test using calcein-acetoxymethyl (calcein-AM) dye that requires very small quantities of cells while ma...... hiện toàn bộ
Utility of flow cytometric detection of CD69 expression as a rapid method for determining poly- and oligoclonal lymphocyte activation
Tập 3 Số 3 - Trang 301-304 - 1996
Patricia Simms, Tom Ellis
CD69 is a lymphoid activation antigen whose rapid expression (< or = 2 h postactivation) makes it amenable for the early detection of T-cell activation and for subset activation analyses. In the present study we evaluated the utility of flow cytometric detection of CD69 expression by T cells activated with polyclonal stimuli (anti-CD3 and staphylococcal enterotoxin B [SEB]) and oligoclo...... hiện toàn bộ
Will immunogenicity limit the use, efficacy, and future development of therapeutic monoclonal antibodies?
Tập 1 Số 4 - Trang 365-372 - 1994
Kristine Kuus-Reichel, Lana S. Grauer, Linda M. Karavodin, Christine Knott, M Krusemeier, Neil E. Kay
While monoclonal antibodies show promise for use in the treatment of a variety of disease states, including cancer, autoimmune disease, and allograft rejection, generation of anti-antibody responses still remains a problem. For example, 50% of the patients who receive OKT3 produce blocking antibodies that interfere with its binding to T cells, thus decreasing the therapeutic effect (51). H...... hiện toàn bộ
T Cells and T-Cell Cytokine Transcripts in the Synovial Membrane in Patients with Osteoarthritis
Tập 5 Số 4 - Trang 430-437 - 1998
Lazaros I. Sakkas, Carla R. Scanzello, Norman A. Johanson, Janet Burkholder, Amit Mitra, Padmini Salgame, Christos D. Katsetos, Chris D. Platsoucas
ABSTRACTThe synovial membrane in osteoarthritis (OA) often exhibits inflammatory infiltrates, but the role of T cells in these infiltrates is not known. T-cell activation antigens were analyzed by immunohistochemistry, and T-cell cytokine transcripts were measured by competitive PCR in synovial membranes from patients with OA and rheumatoid arthritis (RA). Lymphoid...... hiện toàn bộ
Quantitative Fluorogenic PCR Assay for Measuring Ovine Herpesvirus 2 Replication in Sheep
Tập 8 Số 1 - Trang 123-128 - 2001
D. Hüssy, Norbert Stäuber, Christian M. Leutenegger, Stefan Rieder, Mathias Ackermann
ABSTRACT A fluorogenic PCR specific for ovine herpesvirus 2 (OvHV-2) DNA was developed and compared to a previously established conventional seminested PCR. Testing of a total of 152 blood samples from both positive and negative animals revealed that the results of both assays corresponded to each other in 100% of the cases. A second fluorogenic PCR for g...... hiện toàn bộ
A Flow Cytometric Opsonophagocytic Assay for Measurement of Functional Antibodies Elicited after Vaccination with the 23-Valent Pneumococcal Polysaccharide Vaccine
Tập 6 Số 4 - Trang 581-586 - 1999
Joseph Martinez, Sandra Romero‐Steiner, Tamara Pilishvili, Suzanne Barnard, Joseph Schinsky, David Goldblatt, George M. Carlone
ABSTRACTOpsonophagocytosis is the primary mechanism for clearance of pneumococci from the host, and the measurement of opsonophagocytic antibodies appears to correlate with vaccine-induced protection. We developed a semiautomated flow cytometric opsonophagocytosis assay using HL-60 granulocytes as effector cells and nonviable 5,6-carboxyfluorescein, succinimidyl es...... hiện toàn bộ
Oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16S-23S rRNA internal transcribed spacers
Tập 2 Số 4 - Trang 448-453 - 1995
Shaun Tyler, Craig A. Strathdee, K. R. Rozee, W M Johnson
Universal primers targeting conserved sequences flanking the 3' end of the 16S and the 5' end of the 23S rRNA genes (rDNAs) were used to amplify the 16S-23S rDNA internal transcribed spacers (ITS) from eight species of pseudomonads which have been associated with human infections. Amplicons from reference strains of Pseudomonas aeruginosa, Pseudomonas cepacia, Pseudomonas gladioli, Pseudom...... hiện toàn bộ
A New Method with General Diagnostic Utility for the Calculation of Immunoglobulin G Avidity
Tập 6 Số 5 - Trang 725-728 - 1999
Maria H. Korhonen, John Brunstein, Heikki Haario, Alexei Katnikov, R Rescaldani, Klaus Hedman
ABSTRACT The reference method for immunoglobulin G (IgG) avidity determination includes reagent-consuming serum titration. Aiming at better IgG avidity diagnostics, we applied a logistic model for the reproduction of antibody titration curves. This method was tested with well-characterized serum panels for cytomegalovirus, Epstein-Barr virus,...... hiện toàn bộ
Diagnosis of Rickettsial Diseases Using Samples Dried on Blotting Paper
Tập 6 Số 4 - Trang 483-488 - 1999
Didier Raoult
ABSTRACTThe use of filter paper is an inexpensive and convenient method for collecting, storing, and transporting blood samples for serological studies. In addition, samples occupy little space and can be readily transported without refrigeration. Rickettsial diseases often evolve according to an epidemic mode and are now considered reemerging diseases, especially ...... hiện toàn bộ