American Society for Microbiology
SCOPUS (1994-2005)
1071-412X
1098-6588
Cơ quản chủ quản: N/A
Các bài báo tiêu biểu
Inhibition of the Activities of Matrix Metalloproteinases 2, 8, and 9 by Chlorhexidine
Tập 6 Số 3 - Trang 437-439 - 1999
ABSTRACT Matrix metalloproteinases (MMPs) are a host cell-derived proteolytic
enzyme family which plays a major role in tissue-destructive inflammatory
diseases such as periodontitis. The aim of the present study was to evaluate the
inhibitory effect of chlorhexidine (CHX) on MMP-2 (gelatinase A), MMP-9
(gelatinase B), and MMP-8 (collagenase 2) activity. Heat-denatured type I
collagen (gelatin) wa...
Calcein-Acetyoxymethyl Cytotoxicity Assay: Standardization of a Method Allowing Additional Analyses on Recovered Effector Cells and Supernatants
Tập 8 Số 6 - Trang 1131-1135 - 2001
ABSTRACTCytotoxicity assays provide an in vitro evaluation of the lytic activity
of NK and T cells against tumors or transformed cells. However, none of these
methods allow the recovery of cells or supernatants after the assay. We
standardized a microcytotoxicity test using calcein-acetoxymethyl (calcein-AM)
dye that requires very small quantities of cells while maintaining the same
sensitivity as...
Utility of flow cytometric detection of CD69 expression as a rapid method for determining poly- and oligoclonal lymphocyte activation
Tập 3 Số 3 - Trang 301-304 - 1996
CD69 is a lymphoid activation antigen whose rapid expression (< or = 2 h
postactivation) makes it amenable for the early detection of T-cell activation
and for subset activation analyses. In the present study we evaluated the
utility of flow cytometric detection of CD69 expression by T cells activated
with polyclonal stimuli (anti-CD3 and staphylococcal enterotoxin B [SEB]) and
oligoclonal stimuli...
Will immunogenicity limit the use, efficacy, and future development of therapeutic monoclonal antibodies?
Tập 1 Số 4 - Trang 365-372 - 1994
While monoclonal antibodies show promise for use in the treatment of a variety
of disease states, including cancer, autoimmune disease, and allograft
rejection, generation of anti-antibody responses still remains a problem. For
example, 50% of the patients who receive OKT3 produce blocking antibodies that
interfere with its binding to T cells, thus decreasing the therapeutic effect
(51). HAMA resp...
T Cells and T-Cell Cytokine Transcripts in the Synovial Membrane in Patients with Osteoarthritis
Tập 5 Số 4 - Trang 430-437 - 1998
ABSTRACTThe synovial membrane in osteoarthritis (OA) often exhibits inflammatory
infiltrates, but the role of T cells in these infiltrates is not known. T-cell
activation antigens were analyzed by immunohistochemistry, and T-cell cytokine
transcripts were measured by competitive PCR in synovial membranes from patients
with OA and rheumatoid arthritis (RA). Lymphoid cell aggregates, containing
prim...
Quantitative Fluorogenic PCR Assay for Measuring Ovine Herpesvirus 2 Replication in Sheep
Tập 8 Số 1 - Trang 123-128 - 2001
ABSTRACT A fluorogenic PCR specific for ovine herpesvirus 2 (OvHV-2) DNA was
developed and compared to a previously established conventional seminested PCR.
Testing of a total of 152 blood samples from both positive and negative animals
revealed that the results of both assays corresponded to each other in 100% of
the cases. A second fluorogenic PCR for genomic sheep DNA was required to
normalize ...
A Flow Cytometric Opsonophagocytic Assay for Measurement of Functional Antibodies Elicited after Vaccination with the 23-Valent Pneumococcal Polysaccharide Vaccine
Tập 6 Số 4 - Trang 581-586 - 1999
ABSTRACTOpsonophagocytosis is the primary mechanism for clearance of pneumococci
from the host, and the measurement of opsonophagocytic antibodies appears to
correlate with vaccine-induced protection. We developed a semiautomated flow
cytometric opsonophagocytosis assay using HL-60 granulocytes as effector cells
and nonviable 5,6-carboxyfluorescein, succinimidyl ester-labeledStreptococcus
pneumoni...
Oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16S-23S rRNA internal transcribed spacers
Tập 2 Số 4 - Trang 448-453 - 1995
Universal primers targeting conserved sequences flanking the 3' end of the 16S
and the 5' end of the 23S rRNA genes (rDNAs) were used to amplify the 16S-23S
rDNA internal transcribed spacers (ITS) from eight species of pseudomonads which
have been associated with human infections. Amplicons from reference strains of
Pseudomonas aeruginosa, Pseudomonas cepacia, Pseudomonas gladioli, Pseudomonas
mal...
A New Method with General Diagnostic Utility for the Calculation of Immunoglobulin G Avidity
Tập 6 Số 5 - Trang 725-728 - 1999
ABSTRACT The reference method for immunoglobulin G (IgG) avidity determination
includes reagent-consuming serum titration. Aiming at better IgG avidity
diagnostics, we applied a logistic model for the reproduction of antibody
titration curves. This method was tested with well-characterized serum panels
for cytomegalovirus, Epstein-Barr virus, rubella virus, parvovirus B19, and
Toxoplasma gondii . ...
Diagnosis of Rickettsial Diseases Using Samples Dried on Blotting Paper
Tập 6 Số 4 - Trang 483-488 - 1999
ABSTRACTThe use of filter paper is an inexpensive and convenient method for
collecting, storing, and transporting blood samples for serological studies. In
addition, samples occupy little space and can be readily transported without
refrigeration. Rickettsial diseases often evolve according to an epidemic mode
and are now considered reemerging diseases, especially in developing countries,
under co...