Wakame Seaweed Suppresses the Proliferation of 7,12‐Dimethylbenz(a)‐anthracene‐induced Mammary Tumors in Rats

Wiley - Tập 90 Số 9 - Trang 922-927 - 1999
Hiroomi Funahashi1, Tomoaki Imai2, Yuji Tanaka2, Kyosuke Tsukamura2, Yasusyuki Hayakawa2, Toyone Kikumori2, T. Mase2, Takahiro Itoh2, Masaya Nishikawa2, H. Hayashi2, Arihiro Shibata2, Yatsuka Hibi2, Masahide Takahashi3, Tatsuhiko Narita4
1Dept. of Surgery II, Nagoya University School of Medicine.
2Department of Surgery II, Nagoya University School of Medicine, 65 Tsurumaicho, Showa-ku, Nagoya 466-8550
3Department of Pathology II, Nagoya University School of Medicine, 65 Tsurumaicho, Showa-ku, Nagoya 466-8550
4Laboratory of Experimental Pathology, Aichi Cancer Center Research Institute, 1‐1 Kanokoden, Chikusa‐ku, Nagoya 464‐8681

Tóm tắt

We examined the anti‐tumor proliferation effects of wakame seaweed on 7,12‐dimethylbenz(a)‐anthracene (DMBA)‐induced rat mammary tumor. DMBA was administered to 8‐week‐old female Sprague‐Dawley rats, and rats which developed mammary tumors were assigned randomly to three groups. Commercial rat feed was used in a control group (group I‐A), and two feed mixtures were prepared, which contained commercial rat feed blended with wakame at 1.0% (group I‐B) and 5.0% (group I‐C) by weight. The respective feeds were given to each group for 8 weeks, and changes in mammary tumor size were compared. At the end of the experiment, mammary tumors and thyroid glands were resected to compare their weights. Serum total iodine and thyroxin (T4) levels were measured. Immunohistochemical studies for bromodeoxyuridine (BrdU) labeling, transforming growth factor (TGF)‐β, and apoptosis were carried out in the resected tumor. Significant suppression of tumor growth was observed in groups I‐B and I‐C compared with I‐A. In groups I‐B and I‐C, the weights of resected mammary tumors were significantly lower and serum total iodine concentration was significantly higher than in I‐A. BrdU indices were significantly lower in groups I‐B and I‐C, compared with I‐A. TGF‐β and apoptotic index were inversely related to BrdU. These results suggest that iodine is transported from the serum into mammary tissues and induces apoptosis through the expression of TGF‐β. In conclusion, wakame suppressed the proliferation of DMBA‐induced mammary tumors.

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Tài liệu tham khảo

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Wiley

Tập 90 Số 9

922-927

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