Use of differential display analysis to assess the effect of human cytomegalovirus infection on the accumulation of cellular RNAs: Induction of interferon-responsive RNAs

Hua Zhu1, Jiang-Ping Cong1, Thomas Shenk1
1Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014

Tóm tắt

We used differential display analysis to identify mRNAs that accumulate to enhanced levels in human cytomegalovirus-infected cells as compared with mock-infected cells. RNAs were compared at 8 hr after infection of primary human fibroblasts. Fifty-seven partial cDNA clones were isolated, representing about 26 differentially expressed mRNAs. Eleven of the mRNAs were virus-coded, and 15 were of cellular origin. Six of the partial cDNA sequences have not been reported previously. All of the cellular mRNAs identified in the screen are induced by interferon α. The induction in virus-infected cells, however, does not involve the action of interferon or other small signaling molecules. Neutralizing antibodies that block virus infection also block the induction. These RNAs accumulate after infection with virus that has been inactivated by treatment with UV light, indicating that the inducer is present in virions. We conclude that human cytomegalovirus induces interferon-responsive mRNAs.

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Tài liệu tham khảo

A D Yurochko, E-S Hwang, L Rasmussen, S Keay, L Pereira, E-S Huang J Virol 71, 5051–5059 (1997).

B Nowak, A Gmiener, P Sarnow, A J Levine, B Fleckenstein Virology 134, 91–102 (1984).

C Roby, W Gibson J Virol 59, 714–727 (1986).

B Ruger, S Klages, B Walla, J Albrecht, B Fleckenstein, P Tomlinson, B Barrell J Virol 61, 446–453 (1987).

B Lu, M F Stinski J Virol 66, 4434–4444 (1992).

M C Pizzorno, P O’Hare, L Sha, R L LaFemina, G S Hayward J Virol 62, 1167–1179 (1988).

C L Malone, D H Vesole, M F Stinski J Virol 64, 1498–1506 (1990).

R M Stenberg, J Fortney, S W Barlow, B P Magrane, J A Nelson, P Ghazal J Virol 64, 1556–1565 (1990).

K Klucher, M Sommer, J T Kadonaga, D H Spector Mol Cell Biol 13, 1238–1250 (1993).

D M Lukac, J R Manuppello, J C Alwine J Virol 68, 5184–5193 (1994).

F M Jault, J-M Jault, F Ruchti, E A Fortunato, C Clark, J Corbeil, D D Richman, D H Spector J Virol 69, 6697–6704 (1995).

W A Bresnahan, I Boldogh, E A Thompson, T Albrecht Virology 224, 150–160 (1996).

M Lu, T Shenk J Virol 70, 8850–8857 (1996).

D Dittmer, E S Mocarski J Virol 71, 1629–1634 (1997).

E S Mocarski Fields Virology, eds B N Fields, D M Knipe, P M Howley (Lippincott, 3rd Ed., Philadelphia), pp. 2447–2492 (1995).

P Liang, A B Pardee Science 257, 967–971 (1992).

P Liang, D Bauer, L Averboukh, P Warthoe, M Rohrwild, H Muller, M Strauss, A B Pardee Methods Enzymol 254, 304–321 (1995).

S D Elek, H Stern Lancet 1, 1–5 (1974).

S A Plotkin, J Farquhar, E Hornberger J Infect Dis 134, 470–475 (1976).

G V Quinnan, M Delery, A H Rook, W R Frederick, J S Epstein, J F Manischewitz, L Jackson, K M Ramsey, K Mittal, S A Plotkin, M R Hilleman Ann Intern Med 101, 478–483 (1984).

N C Jones, T Shenk Cell 17, 683–689 (1979).

C J Baldick, T Shenk J Virol 70, 6097–6105 (1996).

F Yao, R Courtney J Virol 66, 2709–2716 (1992).

D Levy, A Larner, A Chaudhuri, L E Babiss, J E Darnell Proc Natl Acad Sci USA 83, 8929–8933 (1986).

N Nomura, T Nagase, T Sazuka, A Tanaka, S Sato, N Seki, Y Kawarabayasi, K Ishikawa, S Tabata DNA Res 1, 223–229 (1994).

J Bereta, M Bereta Biochem Biophys Res Comm 217, 363–369 (1995).

Y S Cheng, M F Becker-Manley, T P Chow, D C Horan J Biol Chem 260, 15834–15835 (1985).

S L Church Biochim Biophys Acta 1087, 250–252 (1990).

A Kitamura, K Takahashi, A Okajima, N Kitamura Eur J Biochem 224, 877–883 (1994).

U Buwitt, T Flohr, E C Bottger EMBO J 11, 489–496 (1992).

S Shiojiri, R Fukunaga, Y Ichii, Y Sokawa J Biochem 99, 1455–1464 (1986).

Y-S Cheng, C E Patterson, P Staeheli Mol Cell Biol 11, 4717–4725 (1991).

M Aebi, J Fah, N Hurt, C E Samuel, D Thomis, L Bazzigher, J Pavlovic, O Haller, P Staeheli Mol Cell Biol 9, 5062–5072 (1989).

M A Horisberger, G K McMaster, H Zeller, M G Wathelet, J Content J Virol 64, 1171–1181 (1990).

D C Blomstrom, D Fatey, R Kutny, D Korant, E Knight J Biol Chem 261, 8811–8816 (1986).

N Reich, B Evans, D Levy, D Fatey, E Knight, J E Darnell Proc Natl Acad Sci USA 84, 6394–6398 (1987).

J Hiscott, H Nguyen, R Lin Semin Virol 6, 161–173 (1995).

M W Wathen, M F Stinski J Virol 41, 462–477 (1982).

S H McDonough, D H Spector Virology 125, 31–46 (1983).

S H McDonough, S I Staprans, D H Spector J Virol 53, 711–718 (1985).

N Reich, R Pine, D Levy, J E Darnell J Virol 62, 114–119 (1988).

M J Gutch, N C Reich Proc Natl Acad Sci USA 88, 7913–7917 (1991).

D V R Kalvakolanu, S K Bandyopadhyay, M L Harter, G C Sen Proc Natl Acad Sci USA 88, 7459–7463 (1991).

P A Fitzgerald, P Von Wussow, C Lopez J Immunol 129, 819–824 (1982).

M Feldman, P Fitzgerald-Bocarsly J Interferon Res 10, 435–446 (1990).

Q Li, M Feldman, C Harmon, P Fitzgerald-Bocarsly J Interferon Cytokine Res 1, 109–118 (1996).

B Roizman, A E Sears Fields Virology, eds B N Fields, D M Knipe, P M Howley (Lippincott, 3rd Ed., Philadelphia), pp. 2231–2295 (1995).