Update to the Multiplex PCR Strategy for Assignment of <i>mec</i> Element Types in <i>Staphylococcus aureus</i>

Antimicrobial Agents and Chemotherapy - Tập 51 Số 9 - Trang 3374-3377 - 2007
Catarina Milheiriço1, Duarte C. Oliveira1, Hermı́nia de Lencastre2,1
1Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
2Laboratory of Microbiology, The Rockefeller University, New York, New York 10021

Tóm tắt

ABSTRACT Staphylococcal cassette chromosome mec (SCC mec ) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCC mec typing. Here, we describe an update to the multiplex PCR strategy for SCC mec typing that we described in 2002 so that SCC mec types IV and V may be properly identified.

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