Turgor‐sensitive transport in developing seeds of legumes: the role of the stage of development and the use of excised vs. attached seed coats

Plant, Cell and Environment - Tập 9 Số 2 - Trang 133-140 - 1986
P. Wolswinkel1, Ankie Ammerlaan1
1Botanical Laboratory, State University of Utrecht, Lange Nieuwstraat 106, 3512 PN Utrecht, The Netherlands

Tóm tắt

Abstract After removal of the embryo from developing seeds of Vicia faba L. and Pisum sativum L., the ‘empty’ ovules were filled with a substitute medium (pH 5.5) and the effect of the osmolality of this solution on assimilate transport was exandned. In pulse‐labelling experiments with a mixture of [3H]sucrose and [14C]α‐andnoisobutyric acid (AIB), a solute concentration of 400 mol m−3 (100 mol m3− sucrose + 300 mol m−3 mannitol) was too low to maintain sugar and andno acid transport into empty ovules of V. faba in a very early stage of development (embryo dry weight < 100 mg) on the same level as transport into intact ovules within the same fruit. A 550‐mol m−3 solution could maintain the normal rate of transport. In experiments with seeds in a more advanced stage of development (embryo dry weight > 250 mg), transport of labelled sucrose and AIB into empty ovules filled with a 400‐mol m−3 solution was practically equal to transport into intact ovules within the same fruit. Experiments without isotopes, on sugar and andno acid release from the seed coat, confirmed the important role of the osmotic environment. A very low osmolality of the solution (e.g. 50 mol m−3 mannitol) enhanced net efflux of assimilates from excised seed coats and cotyledons, by inhibiting resorption from the apoplast.

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Tài liệu tham khảo

10.1104/pp.72.3.781

Bergmeyer H.U., 1974, Methods of Enzymatic Analysis, 1205

Daie J., 1984, Sugar uptake and phloem loading of sucrose in bean, Plant Physiology, 75

10.1104/pp.77.4.863

10.1093/oxfordjournals.aob.a085415

10.1111/j.1438-8677.1980.tb03328.x

10.1071/PP9840079

10.1071/PP9780321

10.1016/S0044-328X(83)80068-3

10.1016/S0176-1617(84)80102-9

10.1071/PP9800671

10.1104/pp.75.3.846

10.1016/0003-9861(57)90241-2

10.1111/j.1365-3040.1983.tb01275.x

10.1093/jxb/21.4.915

10.1007/BF00385439

10.1007/BF00385440

10.1104/pp.65.5.975

10.1104/pp.67.5.1016

10.1104/pp.72.1.268

10.1093/jxb/18.2.229

10.1007/BF00027290

10.1007/BF01075256

10.1111/j.1399-3054.1984.tb05893.x

10.1093/jxb/36.3.359

10.1111/j.1399-3054.1983.tb04218.x

10.1016/S0044-328X(82)80077-9

Zamski E., 1984, Turgor regulation of sucrose transport in sugarbeet taproot tissue, Plant Physiology, 75, 156

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Plant, Cell and Environment

Tập 9 Số 2

133-140

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