Transcriptome analysis to identify the downstream genes of androgen receptor in dermal papilla cells

BMC Genomic Data - Tập 23 - Trang 1-10 - 2022
Kai Furuya1, So Fujibayashi1, Tao Wu1, Kouhei Takahashi1, Shin Takase1, Ai Orimoto1, Eriko Sugano1, Hiroshi Tomita1, Sayo Kashiwagi2, Tohru Kiyono3, Tsuyoshi Ishii2, Tomokazu Fukuda1
1Graduate School of Science and Engineering, Iwate University, Morioka, Japan
2Rohto Pharmaceutical Co., Kizugawa, Japan
3Exploratory Oncology Research and Clinical Trial Center, Kashiwa, Japan

Tóm tắt

Testosterone signaling mediates various diseases, such as androgenetic alopecia and prostate cancer. Testosterone signaling is mediated by the androgen receptor (AR). In this study, we fortuitously found that primary and immortalized dermal papilla cells suppressed AR expression, although dermal papilla cells express AR in vivo. To analyze the AR signaling pathway, we exogenously introduced the AR gene via a retrovirus into immortalized dermal papilla cells and comprehensively compared their expression profiles with and without AR expression. Whole-transcriptome profiling revealed that the focal adhesion pathway was mainly affected by the activation of AR signaling. In particular, we found that caveolin-1 gene expression was downregulated in AR-expressing cells, suggesting that caveolin-1 is controlled by AR. Our whole transcriptome data is critical resources for discovery of new therapeutic targets for testosterone-related diseases. The comprehensive gene expression profiling were obtained by RNA-Seq analysis about AR negative and AR positive dermal papilla cells. The bioinformatics analysis suggested that caveolin-1 and EGF receptors are the downstream of AR signaling. Our study showed the combination of pinpoint mutant cells and global transcriptome is effective to identify the downstream genes.

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