Tissue specific phenotypes of polyomavirus A and B enhancer transpositions and duplications: Positional and nonpositional effects on replication in lymphoid cells
Tóm tắt
We have extended our studies using the mouse lymphoid cell system to determine if polyomavirus (Py) cell type specificity is a consequence of the simple presence of either the A and B enhancers, or if specificity results from specific combinations or arrangements of the individual enhancer elements. Mouse fibroblasts and several T lymphoid cell lines were transfected with wild-type DNA or genomes containing either a deletion of the B enhancer, a transposition of the A and B enhancers relative to their normal positions, or a tandem duplication of either the A or B enhancers. In some cells activation of DNA replication was dependent on the simple presence of the B enhancer and not upon its position, as all genomes containing a B enhancer replicated efficiently. In other cells, deletion or transposition of the B enhancer abolished DNA replication which suggested that activation depended upon the position of this element. The A enhancer core duplication replicated well in lymphoid cell lines, but duplication of the B enhancer did not yield a generally efficient configuration. We have interpreted these results to suggest that individual enhancer components can interact via specific combinational principles to yield cell type specific effects upon Py DNA replication.
Tài liệu tham khảo
Amati, P., Cell 43, 561–562, 1985.
Wirak, D.O., Chalifour, L.E., Wassarman, P.M., Muller, W.J., Hassell, J.A., & DePamphilis, M.L., Mol Cell Biol 5, 2924–2935, 1985.
Melin, F., Pinon, H., Kress, C., & Blangy, D., J Virol 53, 862–866, 1985.
Melin, F., Pinon, H., Reiss, C., Kress, C., Montreau, N. & Blangy, D., EMBO J 4, 1799–1803, 1985.
Fujimura, F.K., Deininger, P.L., Friedmann, T., & Linney, E., Cell 23, 809–814, 1981.
DeSimone, V., LaMantia, G., Lania, L., & Amati, P., Mol Cell Biol 5, 2142–2146, 1985.
Campbell, B.A. & Villarreal, L.P., Mol Cell Biol 5, 1534–1537, 1985.
Hassell J.A., Muller W.J., and Mueller C.R. in Botchan M., Grodzicker T., and Sharp P.A. (eds.) DNA Tumor Viruses: Control of Gene Expression and Replication. Cold Spring Harbor Laboratory 1986, pp. 561–569.
Herbomel, P., Bourachot, B., & Yaniv, M., Cell 39, 653–662, 1984.
Bohnlein, E., Chowdhury, K. & Gruss, P., Nucleic Acids Res 13, 4789–4809, 1985.
Veldman, G.M., Lupton, S., & Kamen, R., Mol Cell Biol 5, 649–658, 1985.
Herbomel, P., Saragosti, S., Blangy, D., & Yaniv, M., Cell 25, 651–658, 1981.
Hearing, P., & Shenk, T., Cell, 33, 695–703, 1983.
Banerji, J., Olson, L., & Schaffner, W., Cell 33, 729–740, 1983.
Goodbourn, S., Burstein, H., & Maniatis, T., Cell 45, 601–610, 1986.
Weiher, H., Konig, M., & Gruss, P., Science 219, 626–631, 1983.
Ohlsson, H., & Edlund, T., Cell 45, 35–44, 1986.
Weber, F., de, Villiers, J., & Schaffner, W., Cell 36, 983–992, 1984.
Maione, R., Passnanti, C., De, Simone, V., Delli-Bovi, P., Augusti-Tocco, G., & Amati, P., EMBO J 4, 3215–3221, 1985.
Rochford, R., Campbell, B.A., & Villarreal, L.P., Proc Natl Acad Sci USA 84, 449–453, 1987.
Campbell, B.A., & Villarreal, L.P., Mol Cell Biol 6, 2068–2079, 1986.
Endres, R.O., Marrack, P., & Kappler, J.W., J Immunol 131, 1656–1662, 1983.
Maxam, A., & Gilbert, W., Proc Natl Acad Sci USA 74, 560–564, 1977.
Ruley, H.E., & Fried, M., J Virol 47, 233–237, 1983.
Luthman, H., & Magnusson, G., Nucleic Acids Res 11, 1295–1308, 1983.
Southern, E., J Mol Biol 98, 503–517, 1975.
Patel, I., & Bastia, D., Cell 47, 785–792, 1986.
Velcich, A., Kern, F.G., Basilico, C., & Ziff, E., Mol Cell Biol 6, 4019–4025, 1986.
Levine, A.J., Curr Top Microbiol Immunol 101, 1–30, 1982.