Time course of changes in antioxidant activity of milk from dairy cows fed a trehalose‐supplemented diet

Animal Science Journal - Tập 84 Số 1 - Trang 42-47 - 2013
Naoto Aoki1, Kan Sato2, Shuhei Kanda2, Kazuhisa Mukai3, Yoshiaki Obara4, Hideyuki Itabashi5
1Department of Biological Production, Tokyo University of Agriculture and Technology, Fuchu, Japan
2Department of Biological Production, Tokyo University of Agriculture and Technology, Fuchu
3Hayashibara Biochemical Laboratories Inc, Okayama
4Mito Experimental Farm, Meiji Feed Co. Ltd, Ibaraki, Japan
5Faculty of Applied Life Science, Nippon Veterinary and Life Science University, Musashino, Tokyo

Tóm tắt

ABSTRACTThis study was to investigate the time course of changes to the antioxidant activity of milk from cows fed a trehalose‐supplemented diet, and to determine possible underlying mechanisms for observed changes. Six Holstein cows were used, and subjected to two experimental feeding periods consisting of a 1% trehalose‐supplemented diet for 10 days, followed by a basal diet only (no trehalose) for 10 days. 1,1‐Diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging activities in milk were gradually increased during the trehalose supplementation period and were highest at the end of the second period. However, trehalose was not detected in the milk and plasma of dairy cows fed a diet supplemented with trehalose for 10 days, indicating that the increased antioxidant activity in the milk of trehalose‐fed cows is not due to the direct transfer of trehalose to the milk. Plasma DPPH activities exhibited a similar time course to that seen for milk. Relative superoxide dismutase (SOD) activities in the rumen were higher 3 days after the end of trehalose supplementation than at any other time during the experimental periods. These results suggested that the improved antioxidant activity in milk and plasma of cows fed a trehalose‐supplemented diet was due to improved ruminal relative SOD activity.

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Tài liệu tham khảo

10.3168/jds.S0022-0302(04)73180-X

10.3168/jds.2009-2961

Bayaru E, 2001, Effect of fumaric acid on methane production, rumen fermentation and digestibility of cattle fed roughage alone, Animal Science Journal, 72, 139

10.3168/jds.2007-0596

Fulghum RS, 1984, Superoxide dismutase in ruminal bacteria, Applied Environmental Microbiology, 48, 675, 10.1128/aem.48.3.675-677.1984

10.1111/j.1365-2036.2006.03186.x

10.1159/000051527

10.1016/j.nut.2008.07.017

10.1111/j.1740-0929.2006.00366.x

10.1111/j.1740-0929.2007.00471.x

10.1021/jp045906w

10.1021/ja034777e

10.2527/jas1976.434910x

10.3168/jds.S0022-0302(07)71605-3

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Animal Science Journal

Tập 84 Số 1

42-47

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