Thymoquinone is a protective agent that reduces the negative effects of doxorubicin in rat testis

Human and Experimental Toxicology - Tập 39 Số 10 - Trang 1364-1373 - 2020
Emel Öztürk1, Emin Kaymak2, Ali Tuğrul Akın3, Derya Karabulut4, Hümeyra Ünsal4, Birkan Yakan4
1Department of Histology and Embryology, Faculty of Medicine, Harran University, Sanliurfa, Turkey
2Department of Histology and Embryology, Faculty of Medicine, Yozgat Bozok University, Yozgat, Turkey
3Department of Biology, Faculty of Science, Erciyes University, Kayseri, Turkey
4Department of Histology and Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey

Tóm tắt

Background: Doxorubicin (DOX) is used for treatment of many cancer types. Thymoquinone (THQ) is a powerful antioxidant agent used for reducing side effects of several drugs. The aim of this study is to determine possible therapeutic effects of THQ on doxorubicin-induced testicular toxicity in rats. Methods: Rats were divided into five groups ( n = 8): control, THQ, olive oil, DOX (a single dose of 15 mg/kg intraperitoneally (i.p.) on seventh day of the experiment), and DOX + THQ (10 mg/kg THQ per day and 15 mg/kg DOX i.p. on seventh day). Animals were euthanized, and testis tissues were evaluated histopathologically. Caspase 3 and HSP90 immunostaining were performed to determine the expression levels of these proteins among groups. Terminal deoxynucleotidyl transferase 2′-deoxyuridine, 5′-triphosphate nick-end labeling method was used for evaluation of apoptotic index. Moreover, serum testosterone levels and total antioxidant status (TAS) and total oxidant status (TOS) in testicular tissue were measured by ELISA assay. Results: The DOX group had histopathological deterioration compared to the control group. There was an increase in apoptotic index, caspase 3 and HSP90 expressions in the DOX group. While TAS level of the DOX group decreased, TOS level increased when compared with the other groups. Serum testosterone levels in the DOX group decreased compared to the control group. However, there was improvement in testicular tissue in DOX + THQ group compared to the DOX group. There was a decrease in apoptotic index, caspase 3, and HSP90 expressions in DOX + THQ group compared to the DOX group. Testosterone level of DOX + THQ significantly increased compared to the DOX group. Conclusion: We suggest that THQ can be used as a protective agent to reduce the toxic effects of DOX.

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Tài liệu tham khảo

10.1093/humupd/7.4.394

10.1016/j.lfs.2018.03.023

10.1124/mol.104.002634

10.4103/0973-1296.111265

10.1016/j.phymed.2012.06.011

10.2353/ajpath.2010.090222

Ratain MJ ER, 1999, Cancer chemotherapy

10.1016/j.fct.2010.12.005

10.1155/2015/143629

10.1007/s10753-015-0206-1

10.1016/j.bcp.2011.09.029

10.1016/j.phrs.2015.03.011

10.2174/138920309787315202

10.1379/CSC-99r.1

10.1016/j.tplants.2004.03.006

10.1146/annurev.pharmtox.41.1.367

10.1016/j.immuni.2005.02.004

10.1111/and.12281

10.1515/jbcpp-2018-0073

Johnsen SG, 1970, Hormones, 1, 2

10.1111/and.12603

10.1016/j.bbrc.2018.08.090

10.1002/jbt.21965

10.1080/19390211.2017.1335822

Hozayen W, 2012, Int J Food Sci Nutr, 1, 31

10.2131/jts.20.183

10.1111/j.1476-5381.2008.00042.x

10.1016/j.fertnstert.2005.11.035

10.1161/01.CIR.102.5.572

10.1007/s002040050617

10.1158/0008-5472.CAN-05-2004

10.1042/bj3260001

10.1177/1534734605280018

Minerly AE, 2008, Ethn Dis, 18

10.3748/wjg.v13.i13.1953

10.1006/bbrc.2001.5427

10.1016/j.bbrc.2006.07.028

10.1016/j.ygcen.2015.09.006

Em A, 2009, Iran J Cancer Preve, 2, 175

Mishra O, 2007, J Cell Tissue Res, 7, 861

10.1016/j.celrep.2019.05.092

10.1177/0748233714548474

10.1111/and.12424

10.1111/and.12469

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Human and Experimental Toxicology

Tập 39 Số 10

1364-1373

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