The preparation of monoclonal antibodies which react preferentially with human bone alkaline phosphatase and not liver alkaline phosphatase

Clinica Chimica Acta - Tập 186 - Trang 315-320 - 1990
Craig S. Hill1, Robert L. Wolfert1
1Hybritech Inc., San Diego, CAUSA

Tài liệu tham khảo

McComb, 1979, Alkaline phosphatase, 373 Seargeant, 1979, Evidence that three structural genes code for human alkaline phosphatases, Nature, 281, 152, 10.1038/281152a0 Weiss, 1986, Isolation and characterization of a cDNA encoding a human liver/bone/kidney-type alkaline phosphatase, 83, 7182 Moss, 1984, Improved electrophoretic resolution of bone and liver alkaline phosphatases resulting from partial digestion with neuraminidase, Clin Chim Acta, 143, 177, 10.1016/0009-8981(84)90227-4 Onica, 1986, Affinity electrophoresis of human serum alkaline phosphatase isoenzymes in agarose gel containing lectin, Clin Chim Acta, 155, 285, 10.1016/0009-8981(86)90248-2 Sinha, 1986, Resolution of alkaline phosphatase isoenzymes in serum by isoelectric focusing in immobilized pH gradients, Clin Chem, 32, 1264, 10.1093/clinchem/32.7.1264 Lawson, 1985, Isolation and preliminary characterization of a monoclonal antibody that interacts preferentially with the liver isoenzyme of human alkaline phosphatase, Clin Chem, 31, 381, 10.1093/clinchem/31.3.381 Bailyes, 1987, The preparation of monoclonal antibodies to human bone and liver alkaline phosphatase and their use in immunoaffinity purification and in studying these enzymes when present in serum, Biochem J, 244, 725, 10.1042/bj2440725 Farley, 1989, Alkaline phosphatase activity from human osteosarcoma cell line SAOS-2: an isoenzyme standard for quantifying skeletal alkaline phosphatase activity in serum, Clin Chem, 35, 223, 10.1093/clinchem/35.2.223 Fedde, 1988, Alkaline phosphatase is an ectoenzyme that acts on micromolar concentrations of natural substrates at physiologic pH in human osteosarcoma (SAOS-2) cells, Arch Biochem Biophys, 264, 400, 10.1016/0003-9861(88)90305-0 Murray, 1987, Characterization of a human osteoblastic osteosarcoma cell line (SAOS-2) with high bone alkaline phosphatase activity, J Bone Min Res, 2, 231, 10.1002/jbmr.5650020310 Kohler, 1975, Continuous cultures of fused cells secreting antibody of pre-defined specificity, Nature (London), 256, 495, 10.1038/256495a0