The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea

Proceedings of the National Academy of Sciences of the United States of America - Tập 105 Số 3 - Trang 859-864 - 2008
Dario Cantù1, Ariel R. Vicente2, L. Carl Greve3,4, Frances M. Dewey5, A. B. Bennett3,4, John M. Labavitch3,4, Ann Powell3,4
1Departments of Plant Sciences and Viticulture and Enology, University of California, Davis, CA 95616, USA.
2Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CONICET-UNLP) and Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, CP 1900, Argentina; and
3Departments of *Plant Sciences and
4University of Florida, Gainesville, FL
5Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, United Kingdom

Tóm tắt

Fruit ripening is characterized by processes that modify texture and flavor but also by a dramatic increase in susceptibility to necrotrophic pathogens, such as Botrytis cinerea . Disassembly of the major structural polysaccharides of the cell wall (CW) is a significant process associated with ripening and contributes to fruit softening. In tomato, polygalacturonase (PG) and expansin (Exp) are among the CW proteins that cooperatively participate in ripening-associated CW disassembly. To determine whether endogenous CW disassembly influences the ripening-regulated increase in necrotropic pathogen susceptibility, B. cinerea susceptibility was assessed in transgenic fruit with suppressed polygalacturonase ( LePG ) and expansin ( LeExp1 ) expression. Suppression of either LePG or LeExp1 alone did not reduce susceptibility but simultaneous suppression of both dramatically reduced the susceptibility of ripening fruit to B. cinerea , as measured by fungal biomass accumulation and by macerating lesion development. These results demonstrate that altering endogenous plant CW disassembly during ripening influences the course of infection by B. cinerea , perhaps by changing the structure or the accessibility of CW substrates to pathogen CW-degrading enzymes. Recognition of the role of ripening-associated CW metabolism in postharvest pathogen susceptibility may be useful in the design and development of strategies to limit pathogen losses during fruit storage, handling, and distribution.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 105 Số 3

859-864

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