The cell surface of Trypanosoma cruzi: cytochemistry and freeze-fracture

Journal of Cell Science - Tập 33 Số 1 - Trang 285-299 - 1978
Wanderley de Souza1, A. Martfnez-Palomo2, Arturo González‐Robles2
1Instituto de Biofisica, Universidade Federal do Rio de Janeiro * , 20.000, Rio de Janeiro, Brazil
2Centro de Investigación del IPN † Departamento de Biologia Celular , , Apartado Postal 14-740, México, D.F.

Tóm tắt

ABSTRACT The ultrastructure of epimastigotes of Trypanosoma cruzi, obtained from, acellular cultures, and bloodstream trypomastigotes, isolated from infected mice, were studied by thin-sectioning and freeze-fracturing techniques. Epimastigotes showed a thin (5 nm) surface coat when stained with ruthenium red, while the surface coat of trypomastigotes was more prominent (15 nm thick). Both P and E faces of the plasma membrane of T. cruzi had roughly the same number of intramembranous particles (IMP) as seen by freeze-fracture. The plasma membrane of bloodstream trypomastigotes had less IMP than epimastigotes. Several differentiations of the plasma membrane were observed. In epimastigotes a cytostome appears as a particle-poor region delimited by a pallisade-like row of adjacent IMP. Bloodstream trypomastigotes did not have a cytostome. Instead, abundant pinocytic vesicles were observed. At the base of the flagellum of epimastigotes a ciliary necklace was found. At this region, the surface coat was differentiated as long, hair-like projections after staining with ruthenium red. The flagellar membrane had less IMP than the body membrane. Clusters of IMP were present on both faces of the flagellar membrane at the flagellar-body adhesion zone of epimastigotes. Linear arrays of IMP were also seen. In bloodstream trypomastigotes clusters of particles were observed both on the flagellar and cell body membranes. Our observations demonstrate the presence of considerable structural variations of the T. cruzi plasma membrane at the two stages of the life cycle studied.

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Tài liệu tham khảo

Branton, 1975, Freeze-etching nomenclature, Science, N.Y, 190, 54, 10.1126/science.1166299

Brooker, 1971, The fine structure of Crithidia fasciculata with special reference to the organelles involved in the ingestion and digestion of proteins, Z. Zellforsch. mikrosk. Anat, 116, 532, 10.1007/BF00335057

Brooker, 1967, The cytostome in trypanosomes and allied flagellates, J. Protozoal, 14, 41

Brown, 1963, The ingestion of protein molecules by blood forms of Trypanosoma rhodesiense, Expl Cell Res, 39, 129, 10.1016/0014-4827(65)90015-7

Camargo, 1964, Growth and differentiation in Trypanosoma cruzi. I. Origin of metacyclic trypanosomes in liquid media, Rev. Inst. Med. Trop. Sào Paulo, 6, 93

Chiari, 1978, Concanavalin A receptors on the cell membrane of Trypanosoma cruzi, Acta trop

De Souza, 1977, Surface charge of Trypanosoma cruzi. Binding of cationized ferritin and measurement of cellular electrophoretic mobility, J. Protozool, 24, 411, 10.1111/j.1550-7408.1977.tb04762.x

De Souza, 1975, An electron microscopic and cytochemical study of the cell coat of Trypanosoma cruzi in tissue cultures, Z. Parasitenk, 46, 179, 10.1007/BF00389875

De Souza, 1976, An electron microscopic study of Herpetomonas sp. (Eeptomonas pessoai), Can. J. Microbiol, 22, 197, 10.1139/m76-027

Easterbrook, 1971, The ultrastructure of Crithidia fasciculata. A freeze-etching study, Can. J. Microbiol, 17, 277, 10.1139/m71-046

Gilula, 1972, The ciliary necklace. A ciliary membrane specialization, J. Cell Biol, 53, 494, 10.1083/jcb.53.2.494

Hogan, 1976, Variation in intramembrane components of Trypanosoma brucei from intact and X-irradiated rats. A freeze-cleave study, J. Protozool, 23, 205, 10.1111/j.1550-7408.1976.tb03757.x

LaNgreth, 1975, Protein uptake and digestion in bloodstream and culture forms of Trypanosoma brucei, J. Protozool, 22, 40, 10.1111/j.1550-7408.1975.tb00943.x

Linder, 1977, Plasma membrane specializations in a trypanosomatid flagellate, J. Ultrastruct. Res, 60, 246, 10.1016/S0022-5320(77)80069-5

Luft, 1971, Ruthenium red and violet. I. Chemistry, purification, methods of use. for electron microscopy and mechanism of action, Anat. Rec, 171, 347, 10.1002/ar.1091710302

Maria, 1972, The fine structure of different bloodstream forms of Trypanosoma cruzi, Ann. trop. Med. Parasit, 66, 423, 10.1080/00034983.1972.11686844

Martinez-Palomo, 1978, Ultrastructural characterization of the cuticule of Onchocerca volvas microfilaria, J. Parasit, 64, 10.2307/3279623

Martinez-Palomo, 1976, Membrane structure of Entamoeba histolytica’. Fine structure of freeze-fractured membranes, J. Ultrastruct. Res, 54, 148, 10.1016/S0022-5320(76)80017-2

Martinez-Palomo, 1976, Topographical differences in the distribution of surface coat components and intramembrane particles. A cytochemical and freeze-fracture study in culture forms of Trypanosoma cruzi, J. Cell Biol, 69, 507, 10.1083/jcb.69.2.507

Meyer, 1973, On the fine structure of Trypanosoma cruzi in tissue cultures of pigment epithelium from the chick embryo. Uptake of melanin granules by the parasite, Protozool, 20, 590, 10.1111/j.1550-7408.1973.tb03580.x

Milder, 1969, The cytostome of Trypanosoma cruzi and T. conorhini, J. Protozool, 16, 730, 10.1111/j.1550-7408.1969.tb02335.x

Nogueira, 1975, Studies on the selective lysis and purification of Trypanosoma cruzi, J. exp. Med, 142, 224, 10.1084/jem.142.1.224

Pinto Da Silva, 1970, Membrane splitting in freeze-etching. Covalently bound ferritin as a membrane marker, J. Cell Biol, 45, 598, 10.1083/jcb.45.3.598

Pinto Da Silva, 1975, Membrane structure and surface coat of Entamoeba histolytica. Topochemistry and dynamics of the cell surface: cap formation and microexudate, J. Cell Biol, 64, 538, 10.1083/jcb.64.3.538

Smith, 1974, Observations of freezefractured membranes of a trypanosome, Tissue & Cell, 6, 223, 10.1016/0040-8166(74)90050-0

Steinert, 1960, The existence of a cytostome and the occurrence of pinocytosis in the trypanosome, Trypanosoma mega, J. Cell Biol, 8, 563, 10.1083/jcb.8.2.563

Vickerman, 1969, The fine structure of Trypanosoma congolense in its bloodstream phase, J. Protozool, 16, 54, 10.1111/j.1550-7408.1969.tb02233.x

Vickerman, 1969, On the surface coat and flagellar adhesion in trypanosomes, J. Cell Sci, 5, 163, 10.1242/jcs.5.1.163

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Journal of Cell Science

Tập 33 Số 1

285-299

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