The Utility of MYB Immunohistochemistry (IHC) in Fine Needle Aspiration (FNA) Diagnosis of Adenoid Cystic Carcinoma (AdCC)
Tóm tắt
Differentiating adenoid cystic carcinoma (AdCC) from other basaloid neoplasm in a fine needle aspiration (FNA) sample can be challenging. Activation of MYB in AdCC by the fusion transcript MYB-NFIB has been recently demonstrated in salivary gland and other organs. The aim of this study is to evaluate the utility of MYB immunohistochemistry (IHC) in distinguishing AdCCs and other basaloid neoplasm in cytology specimens. Eighteen FNA cases, from salivary gland and other sites, and their subsequent surgical resection specimens were included in the study. Eight cases were confirmed AdCC on resection. MYB IHC was performed on slides made from cytology cell block and surgical resection paraffin blocks. Percentage and intensity of nuclear staining in tumor cells was scored as 0 to 3. The staining results were concordant between cytology specimens and their corresponding surgical resection tumors. Strong diffuse nuclear staining (score 3, N = 5) was exclusively observed in AdCC, both in cytology and surgical specimens. Only one pleomorphic adenoma and one poorly differentiated basaloid carcinoma were positive for MYB staining (score 1 to 2). Any degree of nuclear MYB labeling was seen in 100% AdCC cases (N = 8/8) compared with of 20% (N = 2/10) of all other non-AdCC cases (P = < 0.001). The sensitivity and specificity of any degree MYB positivity for AdCC in cytology specimen is 100% and 78%. The sensitivity and specificity of strong diffuse MYB labeling (score 2 to 3) for AdCC is 83% and 100% in cytology specimen. Strong diffuse nuclear staining of MYB is valuable in supporting a cytologic diagnosis of AdCC. However, weak and focal labeling of MYB should be interpreted with caution as it can be seen in benign and other malignant basaloid lesions.
Tài liệu tham khảo
citation_journal_title=Head Neck; citation_title=Adenoid cystic carcinoma: a review of recent advances, molecular targets, and clinical trials; citation_author=PM Dillon, S Chakraborty, CA Moskaluk, PJ Joshi, CY Thomas; citation_volume=38; citation_publication_date=2016; citation_pages=620-627; citation_doi=10.1002/hed.23925; citation_id=CR1
citation_journal_title=Ann Med Surg (Lond); citation_title=Adenoid cystic carcinoma of the breast—discordant size on imaging and pathology: a case report and review of literature; citation_author=S Agafonoff, A Sobolewski, TS Braverman; citation_volume=43; citation_publication_date=2019; citation_pages=1-4; citation_doi=10.1016/j.amsu.2019.04.007; citation_id=CR2
citation_journal_title=Otolaryngol Head Neck Surg; citation_title=Laryngeal adenoid cystic carcinoma: a systematic review; citation_author=E Marchiano, OY Chin, CH Fang, RC Park, S Baredes, JA Eloy; citation_volume=154; citation_publication_date=2016; citation_pages=433-439; citation_doi=10.1177/0194599815621884; citation_id=CR3
citation_journal_title=J Cancer Res Ther; citation_title=Primary adenoid cystic carcinoma of lung: a case report and review of the literature; citation_author=T Bhattacharyya, A Bahl, R Kapoor, A Bal, A Das, SC Sharma; citation_volume=9; citation_publication_date=2013; citation_pages=302-304; citation_doi=10.4103/0973-1482.113399; citation_id=CR4
citation_journal_title=Diagn Cytopathol; citation_title=Fine-needle aspiration in the diagnosis of head and neck lesions: a review and discussion of problems in differential diagnosis; citation_author=LJ Layfield; citation_volume=35; citation_publication_date=2007; citation_pages=798-805; citation_doi=10.1002/dc.20769; citation_id=CR5
citation_journal_title=Diagn Cytopathol; citation_title=Adenoid cystic carcinoma: a study of 19 cases of salivary and extra-salivary tumours diagnosed by fine needle aspiration cytology; citation_author=R Kundu, U Handa, RS Punia, A Dass, V Saini; citation_volume=46; citation_publication_date=2018; citation_pages=1004-1009; citation_doi=10.1002/dc.24075; citation_id=CR6
citation_journal_title=J Am Soc Cytopathol; citation_title=Mucoepidermoid carcinoma, acinic cell carcinoma, and adenoid cystic carcinoma on fine-needle aspiration biopsy and the milan system: an international multi-institutional study; citation_author=JA Miller, D An, K Shafique, S Song, RA Rao, K Viswanathan, E Eykman, A Wiles, SZ Ali, A Field, G Fadda, GA Barkan, LJ Layfield, ED Rossi, CN Powers, MT Siddiqui, I Kholova, Z Baloch, Z Maleki; citation_publication_date=2019; citation_doi=10.1016/j.jasc.2019.04.001; citation_id=CR7
citation_journal_title=Arch Pathol Lab Med; citation_title=Performance characteristics of adenoid cystic carcinoma of the salivary glands in fine-needle aspirates; citation_author=MA Al-Abbadi, L Aziz; citation_volume=140; citation_publication_date=2016; citation_pages=1183; citation_doi=10.5858/arpa.2016-0138-LE; citation_id=CR8
citation_journal_title=Am J Clin Pathol; citation_title=Fine-needle aspiration of epithelial-myoepithelial carcinoma of the parotid gland with prominent adenoid cystic carcinoma-like cribriform features: avoiding a diagnostic pitfall; citation_author=OA Aisagbonhi, MA Tulecke, DC Wilbur, A Goldar-Najafi, S Iqbal, PM Sadow, WC Faquin; citation_volume=146; citation_publication_date=2016; citation_pages=741-746; citation_doi=10.1093/ajcp/aqw128; citation_id=CR9
citation_journal_title=J Cytol; citation_title=Fine needle aspiration cytology of basal cell adenoma of parotid simulating adenoid cystic carcinoma; citation_author=S Pal, PK Mondal, A Sharma, M Sikder; citation_volume=35; citation_publication_date=2018; citation_pages=55-57; citation_doi=10.4103/JOC.JOC_46_16; citation_id=CR10
citation_journal_title=J Oral Pathol Med; citation_title=Molecular signature of salivary gland tumors: potential use as diagnostic and prognostic marker; citation_author=FP Fonseca, M Sena Filho, A Altemani, PM Speight, PA Vargas; citation_volume=45; citation_publication_date=2016; citation_pages=101-110; citation_doi=10.1111/jop.12329; citation_id=CR11
citation_journal_title=Oncologist; citation_title=Clinically integrated molecular diagnostics in adenoid cystic carcinoma; citation_author=J Thierauf, N Ramamurthy, VY Jo, H Robinson, RP Frazier, J Gonzalez, M Pacula, E Dominguez Meneses, V Nose, V Nardi, D Dias-Santagata, LP Le, DT Lin, WC Faquin, LJ Wirth, J Hess, AJ Iafrate, JK Lennerz; citation_publication_date=2019; citation_doi=10.1634/theoncologist.2018-0515; citation_id=CR12
citation_journal_title=Oral Dis; citation_title=t(6;9)(MYB-NFIB) in head and neck adenoid cystic carcinoma: a systematic review with meta-analysis; citation_author=YD Almeida-Pinto, SFDS Costa, BAB Andrade, A Altemani, PA Vargas, LG Abreu, FP Fonseca; citation_volume=25; citation_publication_date=2019; citation_pages=1277-1282; citation_doi=10.1111/odi.12984; citation_id=CR13
citation_journal_title=J Natl Cancer Inst; citation_title=Targeting the oncogenic transcriptional regulator MYB in adenoid cystic carcinoma by inhibition of IGF1R/AKT signaling; citation_author=MK Andersson, MK Afshari, Y Andrén, MJ Wick, G Stenman; citation_publication_date=2017; citation_doi=10.1093/jnci/djx017; citation_id=CR14
citation_journal_title=J Oral Pathol Med; citation_title=Immunohistochemical expression of MYB in salivary gland basal cell adenocarcinoma and basal cell adenoma; citation_author=SL Rooney, RA Robinson; citation_volume=46; citation_publication_date=2017; citation_pages=798-802; citation_doi=10.1111/jop.12617; citation_id=CR15
citation_journal_title=Am J Surg Pathol; citation_title=MYB labeling by immunohistochemistry is more sensitive and specific for breast adenoid cystic carcinoma than MYB labeling by FISH; citation_author=JS Poling, R Yonescu, AP Subhawong, R Sharma, P Argani, Y Ning, A Cimino-Mathews; citation_volume=41; citation_publication_date=2017; citation_pages=973-979; citation_doi=10.1097/PAS.0000000000000878; citation_id=CR16
citation_journal_title=Acta Cytol; citation_title=c-Myb overexpression in cytology smears of tracheobronchial and pulmonary adenoid cystic carcinomas; citation_author=AG Vallonthaiel, D Jain, V Singh, K Kaur, K Madan, V Kumar, VK Iyer, MC Sharma; citation_volume=61; citation_publication_date=2017; citation_pages=77-83; citation_doi=10.1159/000453103; citation_id=CR17
citation_journal_title=Cancer Biol Ther; citation_title=Clinical significance of Myb protein and downstream target genes in salivary adenoid cystic carcinoma; citation_author=D Bell, D Roberts, M Karpowicz, EY Hanna, RS Weber, AK El-Naggar; citation_volume=12; citation_publication_date=2011; citation_pages=569-573; citation_doi=10.4161/cbt.12.7.17008; citation_id=CR18
citation_journal_title=Oncotarget; citation_title=Adenoid cystic carcinoma: emerging role of translocations and gene fusions; citation_author=PT Wysocki, E Izumchenko, J Meir, PK Ha, D Sidransky, M Brait; citation_volume=7; citation_publication_date=2016; citation_pages=66239-66254; citation_doi=10.18632/oncotarget.11288; citation_id=CR19
citation_journal_title=Cancer Discov; citation_title=Recurrent fusions in MYB and MYBL1 define a common, transcription factor-driven oncogenic pathway in salivary gland adenoid cystic carcinoma; citation_author=KJ Brayer, CA Frerich, H Kang, SA Ness; citation_volume=6; citation_publication_date=2016; citation_pages=176-187; citation_doi=10.1158/2159-8290.CD-15-0859; citation_id=CR20
citation_journal_title=Nat Genet; citation_title=An oncogenic MYB feedback loop drives alternate cell fates in adenoid cystic carcinoma; citation_author=Y Drier, MJ Cotton, KE Williamson, SM Gillespie, RJ Ryan, MJ Kluk, CD Carey, SJ Rodig, LM Sholl, AH Afrogheh, WC Faquin, L Queimado, J Qi, MJ Wick, AK El-Naggar, JE Bradner, CA Moskaluk, JC Aster, B Knoechel, BE Bernstein; citation_volume=48; citation_publication_date=2016; citation_pages=265-272; citation_doi=10.1038/ng.3502; citation_id=CR21
citation_journal_title=Mod Pathol; citation_title=Analysis of MYB expression and MYB-NFIB gene fusions in adenoid cystic carcinoma and other salivary neoplasms; citation_author=LB Brill, WA Kanner, A Fehr, Y Andrén, CA Moskaluk, T Löning, G Stenman, HF Frierson; citation_volume=24; citation_publication_date=2011; citation_pages=1169-1176; citation_doi=10.1038/modpathol.2011.86; citation_id=CR22
citation_journal_title=Development; citation_title=The WNT/MYB pathway suppresses KIT expression to control the timing of salivary proacinar differentiation and duct formation; citation_author=S Matsumoto, T Kurimoto, MM Taketo, S Fujii, A Kikuchi; citation_volume=143; citation_publication_date=2016; citation_pages=2311-2324; citation_id=CR23
citation_journal_title=Clin Cancer Res; citation_title=Comprehensive analysis of the MYB-NFIB gene fusion in salivary adenoid cystic carcinoma: Incidence, variability, and clinicopathologic significance; citation_author=Y Mitani, J Li, PH Rao, YJ Zhao, D Bell, SM Lippman, RS Weber, C Caulin, AK El-Naggar; citation_volume=16; citation_publication_date=2010; citation_pages=4722-4731; citation_doi=10.1158/1078-0432.CCR-10-0463; citation_id=CR24
citation_journal_title=Eye (Lond); citation_title=Adenoid cystic carcinoma of the lacrimal gland is frequently characterized by MYB rearrangement; citation_author=TY Chen, MG Keeney, AV Chintakuntlawar, DL Knutson, S Kloft-Nelson, PT Greipp, JA Garrity, DR Salomao, JJ Garcia; citation_volume=31; citation_publication_date=2017; citation_pages=720-725; citation_doi=10.1038/eye.2016.307; citation_id=CR25
citation_journal_title=J Surg Case Rep; citation_title=Adenoid cystic carcinoma of the breast; citation_author=DN Thomas, A Asarian, P Xiao; citation_volume=2019; citation_publication_date=2019; citation_pages=rjy355; citation_doi=10.1093/jscr/rjy355; citation_id=CR26