The Herbicide Saflufenacil (Kixor™) is a New Inhibitor of Protoporphyrinogen IX Oxidase Activity

Weed Science - Tập 58 Số 1 - Trang 1-9 - 2010
Klaus Großmann1, Ricarda Niggeweg1, Nicole Christiansen2, Ralf Looser2, Thomas Ehrhardt1
1BASF Agricultural Center Limburgerhof, D-67117 Limburgerhof, Germany
2Metanomics GmbH, Tegeler Weg 33, D-10589 Berlin, Germany

Tóm tắt

Saflufenacil (Kixor™) is a new herbicide of the pyrimidinedione chemical class for preplant burndown and selective preemergence dicot weed control in multiple crops, including corn. In this study, the mode of action of saflufenacil was investigated. For initial characterization, a series of biotests was used in a physionomics approach for comprehensive physiological profiling of saflufenacil effects. With the use of treated duckweed plants, metabolite profiling was performed based on quantification of metabolite changes, relative to untreated controls. Physiological and metabolite profiling suggested a mode of action similar to inhibitors of protoporphyrinogen IX oxidase (PPO) in tetrapyrrole biosynthetic pathway. Saflufenacil inhibited PPO enzyme activity in vitro with 50% inhibition of 0.4 nM for the enzymes isolated from black nightshade, velvetleaf, and corn. PPO inhibition by saflufenacil caused accumulations of protoporphyrin IX (Proto) and hydrogen peroxide (H2O2) in leaf tissue of black nightshade and velvetleaf. In corn, only slight increases in Proto and H2O2 were found, which reflects in planta tolerance of this crop. The results show that saflufenacil is a new PPO-inhibiting, peroxidizing herbicide.

Từ khóa


Tài liệu tham khảo

Grossmann, 2001, Triaziflam and diaminotroazine derivatives affect enantioselectively multiple herbicide target sites, Z. Naturforsch. Sect. C J. Biosci., 56, 559, 10.1515/znc-2001-7-814

Beale, 1990, Biosynthesis of Heme and Chlorophyll, 287

Liebl R. , Walter H. , Bowe S. J. , Holt T. J. , and Westberg D. E. 2008. BAS 800H: a new herbicide for preplant burndown and preemergence dicot weed control. Weed Science Society of America Conf., Abstract 120.

10.1039/b208254n

Walk T. , Looser R. , Bethan B. , Herold M. M. , Kamlage B. , Schmitz O. , van Ravenzwaay B. , Mellert W. , Coelho P. C. G. , Ehrhardt T. , Wiemer J. , Prokoudine A. , and Krennrich G. 2007. Means and methods for analyzing a sample by means of chromatography–mass spectrometry. Intl. Patent WO 2007/012643.

10.1016/j.toxlet.2007.05.021

10.1046/j.1365-313x.2000.00774.x

Diuron, Sigma-Aldrich Chemie, 82041 Deisenhofen, Germany.

10.1023/A:1005903403926

10.1002/ps.923

Bifenox, Dr. Ehrensdorfer, 86199 Augsburg, Germany.

10.1007/978-3-642-59416-8_8

Epifluorescence microscope, Olympus, 20097 Hamburg, Germany.

10.1016/S0021-9673(03)00506-5

Matringe M. , Camadro J. M. , and Brouillet N. 1993. Protoporphyrinogen oxidase, the molecular target site for peroxidizing herbicides. Proc. Brighton Crop Protection Conf.—Weeds. 703–711.

Sigma-Aldrich Chemie, 82041 Deisenhofen, Germany.

10.1016/S1570-0232(03)00498-7

Mitochondrial potential sensor dye JC-1, Invitrogen, 76131 Karlsruhe, Germany.

Dayan F. E. and Duke S. O. 1997. Overview of protoporphyrinogen oxidase-inhibiting herbicides. Proc. Brighton Crop Protection Conf.—Weeds. 83–92.

10.1002/(SICI)1096-9063(199907)55:7<687::AID-PS18>3.0.CO;2-L

10.1038/nrm1451

10.1007/978-3-642-58633-0_11

Oxygen sensor CellOx 325, WTW, 82362 Weilheim, Germany.

10.1002/ps.1016

NovaPac C18 5-µm column, Waters, 65760 Eschborn, Germany.

Triple quadrupole mass spectrometer, Agilent Technologies, 76337 Waldbronn, Germany; Applied Biosystems, 64293 Darmstadt, Germany.

10.1007/978-1-4899-6928-6

Image-analyzing system, LemnaTec, 52146 Würselen, Germany.

10.1073/pnas.082099299

Wakabayashi, 1999, Peroxidizing Herbicides, 164

Scenedesmus acutus Pringsh. cells, Culture Collection of Algae, University of Göttingen, 37073 Göttingen, Germany.

10.1021/bk-1991-0443.ch024

10.1007/978-3-642-59416-8_10

Cleaver cells, DSMZ, Collection of Plant Cell Cultures, 38124 Braunschweig, Germany.

Thông tin
Thông tin xuất bản

Weed Science

Tập 58 Số 1

1-9

Thông tin tác giả