The Enterococcal Surface Protein, Esp, Is Involved in Enterococcus faecalis Biofilm Formation

Applied and Environmental Microbiology - Tập 67 Số 10 - Trang 4538-4545 - 2001
Alejandro Toledo‐Arana1, Jaione Valle1, Cristina Solano1, Marı́a Jesús Arrizubieta1, Carme Cucarella2, Marta Lamata3, Beatriz Amorena1, José Campos3, José R. Penadés2, Íñigo Lasa1
1Instituto de Agrobiotecnologı́a y Recursos Naturales and Departamento de Producción Agraria, Universidad Pública de Navarra-Consejo Superior de Investigaciones Cientı́ficas, Campus de Arrosadia, 31006 Pamplona,1
2Unit of Biochemistry, Department of Basic Biomedical Sciences, Cardenal Herrera-CEU University, 46113 Moncada,2 and
3Department of Microbiology, University Clinics, 31008 Pamplona,3 Spain

Tóm tắt

ABSTRACT The enterococcal surface protein, Esp, is a high-molecular-weight surface protein of unknown function whose frequency is significantly increased among infection-derived Enterococcus faecalis isolates. In this work, a global structural similarity was found between Bap, a biofilm-associated protein of Staphylococcus aureus , and Esp. Analysis of the relationship between the presence of the Esp-encoding gene ( esp ) and the biofilm formation capacity in E. faecalis demonstrated that the presence of the esp gene is highly associated ( P < 0.0001) with the capacity of E. faecalis to form a biofilm on a polystyrene surface, since 93.5% of the E. faecalis esp -positive isolates were capable of forming a biofilm. Moreover, none of the E. faecalis esp -deficient isolates were biofilm producers. Depending on the E. faecalis isolate, insertional mutagenesis of esp caused either a complete loss of the biofilm formation phenotype or no apparent phenotypic defect. Complementation studies revealed that Esp expression in an E. faecalis esp -deficient strain promoted primary attachment and biofilm formation on polystyrene and polyvinyl chloride plastic from urine collection bags. Together, these results demonstrate that (i) biofilm formation capacity is widespread among clinical E. faecalis isolates, (ii) the biofilm formation capacity is restricted to the E. faecalis strains harboring esp , and (iii) Esp promotes primary attachment and biofilm formation of E. faecalis on abiotic surfaces.

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Applied and Environmental Microbiology

Tập 67 Số 10

4538-4545

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