Tautomeric states of the active‐site histidines of phosphorylated and unphosphorylated IIIGlc, a signal‐transducing protein from escherichia coli, using two‐dimensional heteronuclear NMR techniques

Protein Science - Tập 2 Số 4 - Trang 543-558 - 1993
Jeffrey G. Pelton1, Dennis A. Torchia1, Norman D. Meadow2, Saul Roseman2
1Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
2Department of Biology and McCollum-Pratt Institute, The Johns Hopkins University, Baltimore, Maryland 21218

Tóm tắt

AbstractIIIGlc is an 18.1‐kDa signal‐transducing phosphocarrier protein of the phosphoenolpyruvate:glycose phosphotransferase system from Escherichia coli. The 1H, 15N, and 13C histidine ring NMR signals of both the phosphorylated and unphosphorylated forms of IIIGlc have been assigned using two‐dimensional 1H‐15N and 1H‐13C heteronuclear multiple‐quantum coherence (HMQC) experiments and a two‐dimensional 13C‐13C‐1H correlation spectroscopy via JCC coupling experiment. The data were acquired on uniformly 15N‐labeled and uniformly 15N/13C‐labeled protein samples. The experiments rely on one‐bond and two‐bond J couplings that allowed for assignment of the signals without the need for the analysis of through‐space (nuclear Overhauser effect spectroscopy) correlations. The 15N and 13C chemical shifts were used to determine that His‐75 exists predominantly in the Nε2‐H tautomeric state in both the phosphorylated and unphosphorylated forms of IIIGlc, and that His‐90 exists primarily in the Nδ1‐H state in the unphosphorylated protein. Upon phosphorylation of the Nε2 nitrogen of His‐90, the Nδ1 nitrogen remains protonated, resulting in the formation of a charged phospho‐His‐90 moiety. The 1H, 15N, and 13C signals of the phosphorylated and unphosphorylated proteins showed only minor shifts in the pH range from 6.0 to 9.0. These data indicate that the pKa values for both His‐75 and His‐90 in IIIGlc and His‐75 in phospho‐IIIGlc are less than 5.0, and that the pKa value for phospho‐His‐90 is greater than 10. The results are presented in relation to previously obtained structural data on IIIGlc, and implications for proposed mechanisms of phosphoryl transfer are discussed.

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Protein Science

Tập 2 Số 4

543-558

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