A Sánchez-Marroquín1, M. Ledezma1, R. Carreño1
1Facultad de Ciencias, Universidad Central de Venezuela, Apartado 59097, Caracas 104, Venezuela
Tóm tắt
The extracellular production of
l
-lysine in media with cane sugar, blackstrap molasses, or clarified sugar-cane juice by a previously obtained mutant of
Ustilago maydis
was studied. Enzymatically inverted clarified juice (medium J-3) gave 2.9 g of lysine per liter under the following conditions: inoculum, 5%;
p
H 5.8; temperature, 30 C; K
La
in the fermentors, 0.41 mmoles of O
2
per liter per min; fermentation time, 72 hr. The concentrate, obtained by direct evaporation and drying of the fermentation broth, could be used as a possible feed supplement because of its amino-acid and vitamin content.
