Study of Loma acerinae (Microsporidia) detected from three Ponto-Caspian gobies (Gobiidae) in Ukraine
Tóm tắt
A previously recognized Glugea-belonging Microsporidia (G. melanostomi, G. markievichi, G. naidenovi) were found in the intestinal mucosa of Neogobius melanostomus, N. fluviatilis, and Proterorhinchus semilunaris (Teleostei, Gobiidae) from lower Dnieper River and Black Sea coastal inlets, Ukraine. Light microscope observations demonstrated small spherical xenomes about 0.35 mm in diameter filled by developmental stages of parasites. Mature spores were ellipsoidal, slightly narrowed at anterior pole, sometimes ovoid or elongate in shape approximately 3.1–4.5 × 2.0–2.9 μm in size. The stained spores were elongate ovoid that are measured about 2.3–4.3 × 1.6–3.4 μm. Ultrastructural study showed that xenoma wall consisted of finely granular layer up to 350 nm wide. Numerous host-derived collagenous fibers adhered to the xenoma wall were noticed. Microtubular inclusions about 40 nm in diameter occupied the episporontal space. Mature spores displayed the polar filament arranged in one row. One to two last turns of polar filament were sharply tapered to half of diameter. The number of polar filament turns varied depending of host infected from 10 (9 + 1) in N. melanostomus to 13 (11 + 2) in N. fluviatilis and 14 (13 + 1) in P. semilunaris. The endospore thickness varied depending of host infected from 30–35 nm in case of N. fluviatiis to 40–50 nm in P. semilunaris and 60–80 nm in N. melanostomus. SSU 16S rRNA sequence analysis, blast searches, and ultrastuctural data revealed similarity of all studied microsporidians with Loma acerinae (Jirovec, 1930).
Tài liệu tham khảo
Abdel-Azeem S, Abdel-Baki AS, Al-Quraishy S, Rocha S, Dkhil MA, Casal G, Azevedo C (2015) Ultrastructure and phylogeny of Glugea nagelia sp. n. (microsporidia: Glugeidae), infecting the intestinal wall of the yellowfin hind, Cephalopholis hemistiktos (Actinopterygii: Serranidae), from the Red Sea. Folia Parasit 62:007. doi:10.14411/fp.2015.007
Abdel-Ghaffar F, Bashtar AR, Morsy K, Mehlhorn H, Al Quraishy S, Al-Rasheid K, Abdel-Gaber R (2012) Morphological and molecular biological characterization of Pleistophora aegyptiaca sp. nov. infecting the Red Sea fish Saurida Tumbil. Parasitol Res 110:741–752
Arias C, Estevez J, Lores В, Sanmartin МL, Leiro J (1999) Discovery of Loma dimorpha Loubes, Maurant, Gasc, de Buron & Barral1984, in а new host, the shanny, Lipophrys pholis (L.), from the north-west coast of Spain. J Fish Dis 22:155–159
Azevedo C, Matos E (2002) Fine structure of a new species, Loma myrophis (phylum microsporidia), parasite of the Amazonian fish Myrophis Platyrhynchus (Teleostei, Ophichthidae). Eur J Protistol 37(4):445–452. doi:10.1078/0932-4739-00843
Baker MD, Vossbrinck CR, Maddox JVI, Undeen AH (1994) Phylogenetic relationships among Vairimorpha and Nosema species (Microspora) based on ribosomal RNA sequence data. J Invertebr Pathol 30:509–518
Berrebi P (1979) Etude ultrastructurale de Glugea atherinae n. sp., microsporidie parasite de l’athérine Atherina boyeri Risso 1810 (poisson téléostéen) dans les lagunes du Languedoc et de Provence. Z Parasitenkd 60:105–122
Caffara M, Quaglio F, Marcer F, Florio D, Fioravanti ML (2010) Intestinal microsporidiosis in European seabass (Dicentrarchus labrax L.) farmed in Italy. Bull Eur Ass Fish Pathol 30(6):237–240
Casal G, Matos E, Teles-Grilo ML, Azevedo C (2009) Morphological and genetical description of Loma psittaca sp. n. Isolated from the Amazonian fish species Colomesus psittacus. Parasitol Res 105(5):1261–1271
Chernyshenko AS (1960) Parasites of fishes of the Hrygorivsky Estuary. In: Chepurnov VS (ed) Trudy Pervoy Ikhtiologicheskoy Konferentsii po Izucheniyu Morskikh Limanov Severo-Zapadnoy Chasti Chernogo Morya, Chişinău, pp 205–211 (In Russian)
Chernyshenko AS (1966) Parasite fauna of fishes of limans of the north-western Black Sea region (ecology-faunistic review). In: Vodyanitskiy VA, Vinogradov KA, Dolgopolskaya MA, Greze VN, Revin AS, Kalugina AA, Denisenko IF, Delamure SL (eds) Gelmintofauna zhivotnykh yuzhnykh morey. Naukova Dumka, Kiev, pp 105–113 (In Russian)
Faye N, Toguebaye BS, Bouix G (1995) On the cytology and development of Loma boopsi n. Sp. (Microspora, Glugeidae), parasite of Boops boops (Pisces, Teleostei, Sparidae) from the coasts of Senegal. Arch Protistenkd 146(1):85–93
Fomena A, Coste F, Bouix G (1992) Loma camerounensis sp. nov. (protozoa: Microsporida) a parasite of Oreochromis niloticus Linnaeus, 1757 (teleost: Cichlidae) in fish-rearing ponds in Melen, Yaoundé, Cameroon. Parasitol Res 78:201–208
Gasimagomedov AA, Issi IV (1970) Microsporidia parasites of fishes of the Caspian Sea. Trudy Zoologicheskogo lnstituta. lzdatelstvo Academii Nauk SSSR 49:1117–1125 (In Russian)
Krasnovyd V, Kvach Y, Drobiniak O (2012) The parasite fauna of the gobbiid fish (Actinopterygii, Gobiidae) in the Sukhyi Lyman, Black Sea. Vestnik Zoologii 46(6):483–490. doi:10.2478/v10058-012-0041-x
Kvach Y, Boldyrev V, Lohner R, Stepien CA (2015) The parasite community of gobiid fishes (Actinopterygii: Gobiidae) from the lower Volga River region. Biologia 70(7):948–957. doi:10.1515/biolog-2015-0108
Leiro J, Bos J, Peris D, Estevez J, Santamarina МТ, Sanmartin ML (1994) Infection of black goby (Gobius niger L.) from rhe northwest coast of Spain bу Loma dimorpha (protozoa: Microspora). Res Rev Parasitol 54:217–222
Lom J (2002) A catalogue of described genera and species of microsporidians parasitic in fish. Syst Parasitol 53:81–99
Lom J, Nilsen F (2003) Fish microsporidia: fine structural diversity and phylogeny. Int J Parasitol 33:107–127
Lom J, Pekkarinen M (1999) Ultrastructural observations on Loma acerinae (Jirovec, 1930) comb. Nov. (phylum microsporidia). Acta Protozool 38:61–74
Loubès C, Maurand J, Gasc C, Buron I, Barral J (1984) Étude ultrastructurale de Loma dimorpha n. sp., microsporidie parasite de poissons Gobiidae languedociens. Protistologica 30(4):579–589
Matos E, Corral L, Azevedo C (2003) Ultrastructural details of the xenoma of Loma myrophis (phylum microsporidia) and extrusion of the polar tube during autoinfection. Dis Aquat Org 54:203–207
Morrison CM, Spraque V (1981) Electron microscopical study of a new genus and new species of microsporida in the gills of Atlantic cod Gadus morhua L. J Fish Dis 4(1):15–32
Morsy K, Abdel-Ghaffar F, Mehlhorn H, Bashtar AR, Abdel-Gaber R (2012) Ultrastructure and molecular phylogenetics of a new isolate of Pleistophora pagri sp. nov. (microsporidia, Pleistophoridae) from Pagrus pagrus in Egypt. Parasitol Res 111:1587–1597
Naidenova NN (1974) Parasite fauna of fishes of the family Gobiidae of the Black Sea and the sea of Azov. Naukova Dumka, Kiev, p 182 (In Russian)
Ovcharenko NA (1985) Fauna and ecology of microsporidia of Gobiidae in the lower reaches of the Dnepr and the Dnepr-bug and Berezany lagoons. Gidrobiologicheskii Zhurnal 21(4):103–108 (In Russian)
Ovcharenko МO, Yurahno VM (2006) First registration of Loma (microsporidia, Glugeidae) belonging microsporidia in Black Sea fish. Vestnik Zoologii 40(4):38–46 (In Ukrainian)
Rocha S, Azevedo C, Rodrigues P, Araújo MJ and Casal G (2014) Morphology and phylogeny of Glugea atherinae (Microsporidia) infecting the big-scale sand smelt Atherina boyeri (Actinopterygii) in the Minho River, Portugal. XVII Congresso Português de Parasitologia, Lisboa, 20–21 Novembro (Poster)
Takvorian PM, Cali A (1981) The occurrence of Glugea stephani (Hagenmüller, 1899) in american winte flounder, Pseudopleuronectes americanus (Walbaum) from the New York-New Jersey lower bay complex. J Fish Biol 18(4):491–501
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Terry RS, MacNeil C, Dick JTA, Smith JE, Dunn AM (2003) Resolution of a taxonomic conundrum: an ultrastructural and molecular description of the life cycle of Pleistophora mulleri (Pfeiffer 1895; Georgevitch 1929). J Eukaryot Microbiol 50(4):266–273
Vávra J, Maddox JV (1976) Methods in Microsporidiology. In: Bulla JLA, Cheng TC (eds) Comparative pathobiology, vol 1. Plenum Press, New York and London, pp 281–319
Vossbrinck CR, Baker MD, Didier ES, Debrunner-Vossbrinck BA, Shadduck JA (1993) Ribosomal DNA sequences of Encephalitozoon hellem and Encephalitozoon cuniculi: species identification and phylogenetic construction. J Eukaryot Microbiol 40:354–362
Weiss LM, Vossbrinck CR (1998) Ribosomal microsporidiosis: molecular and diagnostic aspects. Adv Parasitol 40:351–395