Structure and Dynamics of the Number of Rat Bone-Marrow Hematopoetic Cells after the Introduction of Magnetoliposomes

Cell and Tissue Biology - Tập 17 - Trang 74-82 - 2023
I. V. Milto1,2, N. M. Shevtsova1, V. V. Ivanova1, O. N. Serebryakova1, R. M. Takhauov1,2, I. V. Sukhodolo1
1Department of Morphology and General Pathology, Siberian State Medical University, Ministry of Health of Russia, Tomsk, Russia
2Seversk Biophysical Research Center, Federal Medical-Biological Agency, Seversk, Russia

Tóm tắt

The use of magnetite nanoparticles in biology and medicine is steadily growing, while some questions regarding their safety remain unclear. For example, nanomagnetite-based magnetoliposomes are a promising basis for the creation of new drugs. In this work, using hematological methods, a comparative assessment of changes in the number of hematopoietic cells in the bone marrow in sexually mature outbred rats was carried out for 120 days after intravenous administration of a suspension of unmodified magnetite nanoparticles and magnetoliposomes based on them. The features of the structure of hematopoietic cells of the main differons of bone marrow (erythrocyte, granulocytic, lymphocytic, monocytic, and platelet) are described, as well as the dynamics of changes in their number during the experiment. Single intravenous administration of a suspension of magnetoliposomes based on magnetite nanoparticles at a dose of 0.14 g (Fe3O4) per 1 kg of body weight (injection volume 3 mL) does not cause changes in the structure and number of hematopoietic cells of the studied rat bone-marrow differons. The absence of a negative effect of magnetoliposomes based on magnetite nanoparticles on the structure of hematopoietic cells of the bone marrow allows us to consider these nanostructures as a promising magnetically controlled carrier for creating modern systems for the targeted delivery of diagnostic and therapeutic drugs.

Tài liệu tham khảo

Bolliger, A.P., Cytologic evaluation of bone marrow in rats: indications, methods, and normal morphology, Vet. Clin. Pathol., 2004, vol. 33, p. 58.

Braham, M.V., Deshantri, A.K., Minnema, M.C., Öner, F.C., Schiffelers, R.M., Fens, M.H., and Alblas, J., Liposomal drug delivery in an in vitro 3D bone marrow model for multiple myeloma, Int. J. Nanomed., 2018, vol. 13, p. 8105.

Chen, S., Chen, S., Zeng, Y., Lin, L., Wu, C., Ke, Y., and Liu, G., Size-dependent superparamagnetic iron oxide nanoparticles dictate interleukin-1β release from mouse bone marrow derived macrophages, J. Appl. Toxicol., 2018, vol. 38, p. 978.

Couto, D., Freitas, M., Vilas-Boas, V., Dias, I., Porto, G., Arturo Lopez-Quintela, M., Rivas, J., Freitas, P., Carvalho, F., and Fernandes, E., Interaction of polyacrylic acid coated and non-coated iron oxide nanoparticles with human neutrophils, Toxicol. Lett., 2014, vol. 225, p. 57.

Couto, D., Sousa, R., Andrade, L., Leander, M., Lopez-Quintela, M.A., Rivas, J., Freitas, P., Lima, M., Porto, G., Porto, B., Carvalho, F., and Fernandes, E., Polyacrylic acid coated and non-coated iron oxide nanoparticles are not genotoxic to human T-lymphocytes, Toxicol. Lett., 2015, vol. 234, p. 67.

Dadachova, E., The effects of nanoparticles on bone marrow cells, in Handbook of Immunological Properties of Engineered Nanomaterials, Singapore: World Sci. Publ., 2013.

Fathy, M.M., Fahmy, H.M., Balah, A.M.M., Moha-med, F.F., and Elshemey, W.M., Magnetic nanoparticles-loaded liposomes as a novel treatment agent for iron deficiency anemia: in vivo study, Life Sci., 2019, vol. 234, p. 1167.

Gaharwar, U.S., Kumar, S., and Rajamani, P., Iron oxide nanoparticle-induced hematopoietic and immunological response in rats, R. Soc. Chem., 2020, vol. 10, p. 35753.

Garcia-Pinel, B., Jabalera, Y., Ortiz, R., Cabeza, L., Jimenez-Lopez, C., Melguizo, C., and Prados, J., Biomimetic magnetoliposomes as oxaliplatin nanocarriers: in vitro study for potential application in colon cancer, Pharmaceutics, 2020, vol. 12, p. 589.

Kelly, C., Jefferies, C., and Cryan, S.-A., Targeted liposomal drug delivery to monocytes and macrophages, J. Drug Deliv., 2011, vol. 2011, p. 727241. https://doi.org/10.1155/2011/727241

Khlusov, I.A., Sedoy, V.S., and Nayden, E.P., The effect of magnetically sensitive nanoscale particles on the pool of hematopoietic stem cells in vitro, Nanosist., Nanomater., N-anotekhnol., 2008, vol. 6, no. 4, p. 1247.

Maladkar, M., Sankar, S., and Yadav, A., A novel approach for iron deficiency anemia with liposomal iron: concept to clinic, J. Biosci. Med., 2020, vol. 8, p. 27.

Mu, C.-F., Shen, J., Liang, J., Zheng, H.-S., Xiong, Y., Wei, Y.-H., and Li, F., Targeted drug delivery for tumor therapy inside the bone marrow, Biomaterials, 2017. https://doi.org/10.1016/j.biomaterials.2017.11.029

Paik, S.-Y.-R., Kim, J.-S., Shin, S.J., and Ko, S., Characterization, quatification, and determination of the toxicity of iron oxide nanoparticles to the bone marrow cells, Int. J. Mol. Sci., 2015, vol. 16, p. 22243. https://doi.org/10.3390/ijms160922243

Pleskova, S.N., Gornostaeva, E.E., Kryukov, R.N., Boryakov, A.V., and Zubkov, S.Yu., Changes in the architectonics and the morphometric characteristics of erythrocytes under the influence of magnetite nanoparticles, Cell Tissue Biol., 2018, vol. 12, p. 127.

Ruiz, A., Ali, L.M.A., Caceres-Velez, P.R., Cornudella, R., Gutierrez, M., Moreno, J.A., Pinol, R., Palacio, F., Fascineli, M.L., de Azevedo, R.B., Morales, M.P., and Millan, A., Hematotoxicity of magnetite nanoparticles coated with polyethylene glycol: in vitro and in vivo study, Toxicol. Res., 2015, vol. 4, p. 1555.

Soenen, S.J., Velde, G.V., Ketkar-Atre, A., Himmel-reich, U., and De Cuyper, M., Magnetoliposomes as magnetic resonance imaging contrast agent, WIREs Nanomed. Nanobiotechnol., 2011, vol. 3, p. 197.

Wu, W., Chen, B., Cheng, J., Wang, J., Xu, W., Liu, L., Xia, G., Wei, H., Wang, X., Yang, M., Yang, L., Zhang, Y., Xu, C., and Li, J., Biocompatibility of Fe3O4/DNR magnetic nanoparticles in the treatment of hematologic malignancies, Int. J. Nanomed., 2010, vol. 5, p. 1079.

Wu, Q.H., Jin, R.R., Feng, T., Liu, L., Yang, L., Tao, Y.H., Anderson, J.M., Ai, H., and Li, H., Iron oxide nanoparticles and induced autophagy in human monocytes, Toxicol. Lett., 2014, vol. 225, p. 57.

Thông tin
Thông tin xuất bản

Cell and Tissue Biology

Tập 17

74-82

Thông tin tác giả