Species‐specific impact of the autophagy machinery on Chikungunya virus infection

EMBO Reports - Tập 14 Số 6 - Trang 534-544 - 2013
Delphine Judith1,2, Serge Mostowy3,4, Mehdi Bouraï5, Nicolas Gangneux1,2, Mickaël Lelek6, Marianne Lucas‐Hourani5, Nadège Cayet7, Yves Jacob8, Marie‐Christine Prévost7, Philippe Pierre9,10, Frédéric Tangy5, Christophe Zimmer6, Pierre Lebon5, Thérèse Couderc1,2, Marc Lecuit1,2,11,12
1Inserm U1117, Institut Pasteur, Biology of Infection Unit,
2Institut Pasteur, Biology of Infection Unit
3Inserm U604, INRA USC2020, Institut Pasteur, Bacteria-Cell Interactions Unit,
4Institut Pasteur, Bacteria-Cell Interactions Unit,
5Institut Pasteur, Viral Genomic and Vaccination Unit,
6CNRS UMR2582, Institut Pasteur, Computational and Imaging and Modeling Group Paris France
7Institut Pasteur, Ultrastructural Microscopy Platform,
8Institut Pasteur, Genetic, Papillomavirus and Human Cancer Unit,
9Centre d'Immunologie de Marseille-Luminy, Universite de la Mediterranee, Parc Scientifique de Luminy Marseille France
10Inserm U631, CNRS UMR6102, Centre d'Immunologie de Marseille-Luminy, Parc Scientifique de Luminy Marseille France
11Necker-Enfants Malades University Hospital, APHP, Division of Infectious Diseases and Tropical Medicine Paris France
12Necker‐Enfants Malades University Hospital, APHP, Division of Infectious Diseases and Tropical Medicine Paris France

Tóm tắt

Chikungunya virus (CHIKV) is a recently re‐emerged arbovirus that triggers autophagy. Here, we show that CHIKV interacts with components of the autophagy machinery during its replication cycle, inducing a cytoprotective effect. The autophagy receptor p62 protects cells from death by binding ubiquitinated capsid and targeting it to autophagolysosomes. By contrast, the human autophagy receptor NDP52—but not its mouse orthologue—interacts with the non‐structural protein nsP2, thereby promoting viral replication. These results highlight the distinct roles of p62 and NDP52 in viral infection, and identify NDP52 as a cellular factor that accounts for CHIKV species specificity.

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EMBO Reports

Tập 14 Số 6

534-544

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