Spatiotemporal analysis of population genetic structure in Geomonhystera disjuncta (Nematoda, Monhysteridae) reveals high levels of molecular diversity

Marine Biology - Tập 151 - Trang 1799-1812 - 2007
S. Derycke1,2, T. Backeljau3,4, C. Vlaeminck1, A. Vierstraete5,2, J. Vanfleteren5,2, M. Vincx1, T. Moens1
1Department of Biology, Marine Biology Section, Ghent University, Ghent, Belgium
2CeMoFe, Ghent University, Ghent, Belgium
3Royal Belgian Institute of Natural Sciences, Brussels, Belgium
4Evolutionary Biology Group, Department of Biology, University of Antwerp, Antwerp, Belgium
5Department of Biology, Ghent University, Ghent, Belgium

Tóm tắt

Species identification in the phylum Nematoda is complicated due to the paucity of easily obtainable diagnostic morphological features. Furthermore, the cosmopolitan distribution of several species despite low dispersal abilities makes cryptic diversity potentially substantial within this phylum. We conducted a population genetic survey in the marine nematode Geomonhystera disjuncta in Belgium and The Netherlands in two seasons. The mitochondrial cytochrome oxidase c subunit 1 (COI) gene was screened with the single-strand conformation polymorphism method in 759 individuals. The 43 haplotypes were grouped into five lineages, with low divergences within (<3%) and high divergences between lineages (>14%). Analysis of the nuclear ITS region yielded concordant tree topologies, indicating the presence of five cryptic taxa within G. disjuncta. Analysis of Molecular Variance (AMOVA) illustrated a significant structuring in all lineages and temporal fluctuations in haplotype frequencies within and between locations. Metapopulation dynamics and/or priority effects best explained this structuring. Finally, our data indicate that the COI gene may be useful for DNA barcoding purposes.

Tài liệu tham khảo

Andrássy I (1981) Revision of the order Monhysterida (Nematoda) inhabiting soil and inland waters. Opus Zool 17–18:13–47

De Coninck LA, Schuurmans Stekhoven JH (1933) The free-living marine nemas of the Belgian coast II. Mém Mus R Hist Nat Belg 58:1–163

Faust MA, Gulledge RA (1996) Associations of microalgae and meiofauna in floating detritus at a mangrove island, Twin Cays, Belize. J Exp Mar Biol Ecol 22:115–123

Felsenstein J (2004) PHYLIP: Phylogeny inference package v 3.6, University of Washington, USA

Gerlach SA (1965) Freilebende Meeresnematoden aus der Gezeitenzone von Spitzbergen. Veröff Inst Meeresforsch Bremerh 9:109–172

Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Lausanne, Switzerland

Huelsenbeck JP, Ronquist F (2005) Mr Bayes v 3.1.2, Bayesian Analysis of Phylogeny. University of California, San Diego and Florida State University

Miller MP (1997) Tools for population genetic analysis (TFPGA) 1.3 A Windows program for the analysis of allozyme and molecular population genetic data

Mokievsky VO, Filippova KA, Chesunov AV (2005) Nematode fauna associated with detached kelp accumulations in the subtidal zone of the White Sea. Oceanology 45:689–697

Nicholas KB, Nicholas HB (1997) Genedoc: a tool for editing and annotating multiple sequence alignments. Distributed by the author

Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University

Schneider S, Roessli D, Excoffier L (2000) Arlequin ver. 2.000: A software for population genetics data analysis. Genetics and Biometry Laboratory, University of Geneva, Switzerland

StatSoft, Inc. (2001). STATISTICA (data analysis software system), version 6. http://www.statsoft.com

Swofford DL (1998) PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland

Ullberg J (2004) Dispersal in free-living, marine, benthic nematodes: passive or active processes? PhD Dissertation, Stockholm University, Sweden, p 27

Vranken G (1987) An autecological study of free-living marine nematodes. Acad Anal- Meded K Acad Belg Kl Wet 49:73–97

Vranken G, Herman PJM, Heip C (1988) Studies of life-history and energetics of marine and brackish-water nematodes. I. Demography of Monhystera disjuncta at different temperature and feeding conditions. Oecologia 77:296–301

Warwick RM, Platt HM, Somerfield PJ (1998) Free-living marine nematodes Part III. Monhysterids. Synopsis of the British fauna 53, Dorset Press, Great Britain