Spatial localization of the stimulus‐induced rise in cytosolic Ca2+ in bovine adrenal chromaffin cells

FEBS Letters - Tập 247 - Trang 429-434 - 1989
Timothy R. Cheek, Antony J. O'Sullivan1, Roger B. Moreton, Michael J. Berridge, Robert D. Burgoyne1
1Department of Physiology, University of Liverpool, Brownlow Hill, PO Box 147, Liverpool L69 3BX, England

Tóm tắt

The spatial distribution of the intracellular free Ca2+ (Ca2+ i) rise elicited by different stimuli in bovine adrenal chromaffin cells was examined in single fura‐2‐loaded cells. In response to the potent secretagogues nicotine and high K+, Ca2+ i was initially localized exclusively to the entire subplasmalemmal area of the cell. In response to the ineffective secretagogues, methacholine and muscarine, the rise in Ca2+ i originated only in one pole of the cell and even at the peak of the response Ca2+ was still generally restricted to this same area of the cell. These results suggest that the triggering of exocytosis from these cells requires a specific spatial distribution of Ca2+ i.

Tài liệu tham khảo

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FEBS Letters

Tập 247

429-434

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