Solubilisation and fractional precipitation of a steroid alpha-ketol oxidase

Journal of Steroid Biochemistry - Tập 23 - Trang 1083-1085 - 1985
I.R. Senciall1, S. Rahal1, K. Sethumadhavan1
1Medical School, Memorial University of Newfoundland, St John's, Newfoundland, Canada A1B 3V6

Tài liệu tham khảo

Dieter, 1982, Functional and structural polymorphism of rabbit microsomal cytochrome P-450 Form 3b, J. biol. Chem., 257, 9315, 10.1016/S0021-9258(18)34071-7 Dieter, 1982, Identification of rabbit microsomal cytochrome P-450 isozyme, Form 1, as a hepatic progesterone 21-hydroxylase, Biochem. biophys. Res. Commun., 105, 515, 10.1016/0006-291X(82)91465-6 Senciall, 1982, A reconstituted hepatic cytochrome P-450 steroid 21-hydroxylase system and its stimulation by cytochrome b5, Vol. 5, 99 Dey, 1978, Acidic steroid metabolites: Biosynthesis of steroid carboxylic acids by rabbit liver microsomes and the involvement of a cytochrome P-450-independent mixed function oxidase, J. steroid Biochem., 9, 1099, 10.1016/0022-4731(78)90039-0 Senciall, 1983, Progesterone C21 hydroxylation and steroid carboxylic acid biosynthesis in the rabbit. in vitro studies with endocrine, metabolic and potential target tissues, Can. J. Biochem. cell Biology, 61, 722, 10.1139/o83-091 Coon, 1978, Two forms of liver microsomal cytochrome P-450, cytochrome P-450LM and P-450LM (rabbit liver), Vol. 52, 109 Lowry, 1951, Protein measurement with Folin phenol reagent, J. biol. Chem., 193, 265, 10.1016/S0021-9258(19)52451-6 Omura, 1964, The carbon monoxide-binding pigment of liver microsomes: Evidence for its hemoprotein nature, J. biol. Chem., 239, 2370, 10.1016/S0021-9258(20)82244-3 Strittmatter, 1956, The isolation and properties of microsomal cytochrome, J. biol. Chem., 221, 253, 10.1016/S0021-9258(18)65245-7 French, 1979, Properties of NADPH-cytochrome P-450 reductase purified from rabbit liver microsomes, Archs biochem. Biophys., 195, 565, 10.1016/0003-9861(79)90383-7 Monder, 1971, Synthesis of steroids containing the α-ketol acidic side chain, Steroids, 18, 187, 10.1016/0039-128X(71)90025-0