Simultaneously both expression of LMP-1 and methylation of E-cadherin: Molecular biomarker in stage IV of nasopharyngeal carcinoma patients

Balkan Journal of Medical Genetics - Tập 24 Số 1 - Trang 57-66 - 2021
Thuan Duc Lao1, Phuong Kim Truong1, Thiều Hồng Huệ1, DH Nguyen2, MT Nguyen3, Thuy Ai Huyen Le1
1Department of Pharmaceutical and Medical Biology, Ho Chi Minh City Open University , Ho Chi Minh City , Vietnam
2Ear, Nose and Throat Department, University of Medicine and Pharmacy at Ho Chi Minh City , Ho Chi Minh City , Vietnam
3Department of Otorhinolaryngology, Cho Ray Hospital , Ho Chi Minh City , Vietnam

Tóm tắt

Abstract The phenome of E-cadherin gene methylation and the expression of latent membrane protein 1 (LMP-1) gene are associated with nasopharyngeal carcinoma (NPC). In order to determine whether cooperative LMP-1 expression or methylation of E-cadherin could serve as the potential molecule biomarker target for diagnosis and therapy of NPC, a case-control study including 93 NPC biopsy samples and 100 non cancerous nasopharyngeal swab samples were examined, as well as the strength of association among them by the quantitative polymerase chain reaction (qPCR) and nested-methylation-specific PCR methods. The significantly higher frequency of LMP-1 expression and E-cadherin methylation in NPC biopsy samples, accounting for 76.34 and 73.12%, respectively, compared to non cancerous samples, accounting for 0.00 and 30.00%, respectively, were observed. The significant correlation between the LMP-1 expression and E-cadherin methylation in NPC samples was reported. In detail, in the stage IV of NPC, in case of LMP-1-positive samples, 35 of 37 samples (accounting for 94.60%) were positive for methylation of E-cadherin. It was demonstrated that cooperative LMP-1 expression and E-cadherin gene methylation could serve as a molecular biomarker in NPC.

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Thông tin xuất bản

Balkan Journal of Medical Genetics

Tập 24 Số 1

57-66

Thông tin tác giả