Simultaneous identification of unmyelinated and myelinated primary somatic afferents by co-injection of isolectin B4 and Cholera toxin subunit B into the sciatic nerve of the rat

Bao, 2002, Peripheral axotomy induces only very limited sprouting of coarse myelinated afferents into inner lamina II of rat spinal cord, Eur J Neurosci, 16, 175, 10.1046/j.1460-9568.2002.02080.x Finkelstein, 1974, Dissociation and recombination of the subunits of the cholera enterotoxin (Choleragen), J Immunol, 113, 145, 10.4049/jimmunol.113.1.145 Fullmer, 2004, Identification of some lectin IB4 binding proteins in rat dorsal root ganglia, Neuroreport, 15, 1705, 10.1097/01.wnr.0000136037.54095.64 Ganser, 1983, Ganglioside localisation on myelinated nerve fibres by cholera toxin binding, J Neurocytol, 12, 921, 10.1007/BF01153342 Gerke, 2004, Ultrastructural analysis of the central terminals of primary sensory neurones labelled by transganglionic transport of Bandeiraea simplicifolia I-isolectin B4, Neuroscience, 127, 165, 10.1016/j.neuroscience.2004.05.008 Goldstein, 1999, The Griffonia simplificifolia I-B4 isolectin. A probe for alpha-d-galactosyl end groups, Subcell Biochem, 19, 789 Jancsó, 2004, Selective C-fiber deafferentation of the spinal dorsal horn prevents lesion-induced transganglionic transport of choleragenoid to the substantia gelatinosa in the rat, Neurosci Lett, 361, 204, 10.1016/j.neulet.2003.12.063 Kitao, 2002, Proliferation patterns of dorsal root ganglion neurons of cutaneous, muscle and visceral nerves in the rat, J Neurocytol, 31, 765, 10.1023/A:1025760116189 Kitchener, 1993, Selective labelling of primary sensory afferent terminals in lamina II of the dorsal horn by injection of Bandeiraea simplicifolia isolectin B4 into peripheral nerves, Neuroscience, 154, 545, 10.1016/0306-4522(93)90274-J Kitchener, 1994, Transganglionic labelling of primary sensory afferents in the rat lumbar spinal cord: comparison between wheatgerm agglutinin and the I-B4 isolectin from Bandeiraea simplicifolia, J Neurocytol, 23, 745, 10.1007/BF01268087 LaMotte, 1991, Central projections of the sciatic, saphenous, median, and ulnar nerves of the rat demonstrated by transganglionic transport of choleragenoid-HRP (B-HRP) and wheat germ agglutinin-HRP (WGA–HRP), J Comp Neurol, 311, 546, 10.1002/cne.903110409 Liu, 1995, Co-injection of wheat germ agglutinin-HRP and choleragenoid-HRP into the sciatic nerve of the rat blocks transganglionic transport, J Histochem Cytochem, 43, 489, 10.1177/43.5.7730587 Llewellyn-Smith, 1992, Complete penetration of antibodies into vibratome sections after glutaraldehyde fixation and ethanol treatment: light and electron microscopy for neuropeptides, J Histochem Cytochem, 40, 1741, 10.1177/40.11.1431060 Ribeiro-da-Silva, 2004, Substantia gelatinosa of the spinal cord, 129 Rivero-Melián, 1990, Distribution of lumbar dorsal root fibres in the lower thoracic and lumbosacral spinal cord of the rat studied with choleragenoid horseradish peroxidase conjugate, J Comp Neurol, 299, 470, 10.1002/cne.902990407 Rivero-Melián, 1991, Choleragenoid horseradish peroxidase used for studying projections of some hindlimb cutaneous nerves and plantar foot afferents to the dorsal horn and Clarke's column in the rat, Exp Brain Res, 84, 125, 10.1007/BF00231767 Robertson, 1985, A comparison between wheatgerm agglutinin and choleragenoid-horseradish peroxidase as anterogradely transported markers in central branches of primary sensory neurones in the rat with some observations in the cat, Neuroscience, 14, 895, 10.1016/0306-4522(85)90152-6 Robertson, 1991, Populations of rat spinal primary afferent neurons with choleragenoid binding compared with those labelled by markers for neurofilament and carbohydrate groups: a quantitative immunocytochemical study, J Neurocytol, 20, 387, 10.1007/BF01355535 Sántha, 2003, Transganglionic transport of choleragenoid by capsaicin-sensitive C-fibre afferents to the substantia gelatinosa of the spinal dorsal horn after peripheral nerve section, Neuroscience, 116, 621, 10.1016/S0306-4522(02)00701-7 Scott, 1990, Lectin binding identifies a subpopulation of neuronsin the chick dorsal root ganglia, J Neurosci, 10, 336, 10.1523/JNEUROSCI.10-01-00336.1990 Shehab, 1986, The origins of the sciatic nerve and changes in neuropeptides after axotomy: a double labelling study using retrograde transport of true blue and vasoactive intestinal polypeptide immunohistochemistry, Brain Res, 376, 180, 10.1016/0006-8993(86)90914-5 Shehab, 2003, Evidence against cholera toxin B subunit as a reliable tracer for sprouting of primary afferents following peripheral nerve injury, Brain Res, 964, 218, 10.1016/S0006-8993(02)04001-5 Shehab, 2004, Do central terminals of intact myelinated primary afferents sprout into the superficial dorsal horn of rat spinal cord after the injury of a neighbouring peripheral nerve?, J Comp Neurol, 474, 427, 10.1002/cne.20147 Shehab, 2008, Unmyelinated primary afferents from adjacent spinal nerves intermingle in the spinal dorsal horn: a possible mechanism contributing to neuropathic pain, Brain Res, 1208, 111, 10.1016/j.brainres.2008.02.089 Shehab, 2009, Acute and chronic sectioning of fifth lumbar spinal nerve has equivalent effects on the primary afferents of sciatic nerve in rat spinal cord, J Comp Neurol, 517, 481, 10.1002/cne.22163 Sugiura, 1986, Central projections of identified, unmyelinated (C) afferent fibers innervating mammalian skin, Science, 234, 358, 10.1126/science.3764416 Sugiura, 1989, Difference in distribution of central terminals between visceral and somatic unmyelinated (C) primary afferent fibers, J Neurophysiol, 62, 834, 10.1152/jn.1989.62.4.834 Swett, 1985, The somatotopic organization of primary afferent terminals in the superficial laminae of the dorsal horn of the rat spinal cord, J Comp Neurol, 231, 66, 10.1002/cne.902310106 Tong, 1999, Increased uptake and transport of cholera toxin B-subunit in dorsal root ganglion neurons after peripheral axotomy: possible implications for sensory sprouting, J Comp Neurol, 404, 143, 10.1002/(SICI)1096-9861(19990208)404:2<143::AID-CNE1>3.0.CO;2-# Trojanowski, 1982, Horseradish peroxidase (HRP) conjugates of cholera toxin and lectins are more sensitive retrogradely transported markers than free HRP, Brain Res, 231, 33, 10.1016/0006-8993(82)90005-1 Valtschanoff, 1992, Peripheral injury and anterograde transport of wheat germ agglutinin-horse radish peroxidase to the spinal cord, Neuroscience, 3, 685, 10.1016/0306-4522(92)90457-D Wang, 1994, Transganglionic transport and binding of the isolectin B4 from Griffonia simplicifolia I in rat primary sensory neurons, Neuroscience, 62, 539, 10.1016/0306-4522(94)90387-5 Weinberg, 1990, Extracellular labeling of unmyelinated dorsal root terminals after WGA-HRP injections in spinal ganglia, Brain Res, 23, 351, 10.1016/0006-8993(90)91513-G Willis, 1991 Woolf, 1992, Peripheral nerve injury triggers central sprouting of myelinated afferents, Nature, 355, 75, 10.1038/355075a0