Simultaneous High-Performance Thin-Layer Chromatographic Quantification of Withaferin A and Withanolide A in Solanum nigrum L. “Black Nightshade”

A simple, selective, sensitive, and precise high-performance thin-layer chromatographic technique was followed to determine the diversity within accessions of Solanum nigrum. Fruit, stem, leaf, and root samples extracted in methanol were used to quantify withanolide A and withaferin A. Significant difference has been detected among the different plant parts. The maximum amount of withaferin A has been found to be present in the leaf sample (71.65 ± 2.86 mg g−1 DW), followed by the stem sample, while the least amount of withaferin A has been detected in the mature fruit sample (5.97 ± 1.91 mg g−1 DW).