Sequence Diversity of the Intergenic Spacer Region of the rRNA Gene of Malassezia globosa Colonizing the Skin of Patients with Atopic Dermatitis and Healthy Individuals

Journal of Clinical Microbiology - Tập 41 Số 7 - Trang 3022-3027 - 2003
Takashi Sugita1, Minako Kodama2, Masuyoshi Saito3, Tomonobu Ito3, Yukihiko Kato3, Ryoji Tsuboi3, Akemi Nishikawa2
1Department of Microbiology
2Department of Immunobiology, Meiji Pharmaceutical University, Kiyose
3Department of Dermatology, Tokyo Medical University, Shinjuku-ku, Tokyo, Japan

Tóm tắt

ABSTRACT The lipophilic yeast Malassezia globosa is one of the major constituents of the mycoflora of the skin of patients with atopic dermatitis (AD). We compared the genotypes of M. globosa colonizing the skin surface of 32 AD patients and 20 healthy individuals for polymorphism of the intergenic spacer (IGS) 1 region of the rRNA gene. Sequence analysis demonstrated that M. globosa was divided into four major groups, which corresponded to the sources of the samples, on the phylogenetic tree. Of the four groups, two were from AD patients and one was from healthy subjects. The remaining group included samples from both AD patients and healthy subjects. In addition, the IGS 1 region of M. globosa contained short sequence repeats: (CT) n , and (GT) n . The number of sequence repeats also differed between the IGS 1 of M. globosa from AD patients and that from healthy subjects. These findings suggest that a specific genotype of M. globosa may play a significant role in AD, although M. globosa commonly colonizes both AD patients and healthy subjects.

Từ khóa


Tài liệu tham khảo

10.1128/CMR.15.1.21-57.2002

10.1023/A:1007017917230

10.1128/JCM.36.9.2413-2418.1998

10.1128/JCM.39.11.4076-4081.2001

10.1016/S0723-2020(00)80028-4

Downing D. T. M. E. Stewart and J. S. Strauss. 1999. Lipids of the epidermis and the sebaceous glands p. 144-155. In I. M. Freedberg A. Z. Eisen K. Wolff K. F. Austen L. A. Goldsmith S. I. Katz and T. B. Fitzpatrick (ed.) Fitzpatrick's dermatology in general medicine 5th ed. McGraw-Hill New York N.Y.

10.1128/CMR.15.4.545-563.2002

10.1111/j.1558-5646.1985.tb00420.x

10.1007/BF00399623

10.1080/mmy.39.3.243.251

Hanifin, J. M., and G. Rajka. 1980. Diagnostic features of atopic dermatitis. Acta Dermato-Venereol.92:4-47.

10.1046/j.1523-1747.2000.00072.x

10.1007/BF01731581

10.1016/S0923-1811(01)00089-5

10.1111/j.1348-0421.2000.tb02508.x

10.1016/S0923-1811(02)00020-8

10.1128/jcm.35.5.1216-1223.1997

10.1099/00222615-40-5-358

10.1111/j.1574-695X.1998.tb01116.x

10.1016/S0923-1811(01)00101-3

10.1080/mmy.38.5.337.341

Saitou, N., and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol.4:406-425.

10.1128/JCM.37.6.1985-1993.1999

10.1111/j.1348-0421.2001.tb01312.x

10.1128/JCM.39.10.3486-3490.2001

10.1128/JCM.40.5.1826-1830.2002

10.1128/JCM.40.4.1363-1367.2002

10.1093/nar/22.22.4673

10.1007/s000180050019

10.1128/aem.63.12.4645-4650.1997

10.1007/BF01106105

Thông tin
Thông tin xuất bản

Journal of Clinical Microbiology

Tập 41 Số 7

3022-3027

Thông tin tác giả