Selection of endogenous 13C substrates for observation of intracellular metabolism using the dynamic nuclear polarization technique

Radiation Medicine - Tập 28 - Trang 173-179 - 2010
Masafumi Harada1, Hitoshi Kubo1, Takamasa Abe2, Hiroshi Maezawa3, Hideki Otsuka4
1Department of Medical Imaging, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan
2MRI/Biotools Division, Oxford Instruments KK, Tokyo, Japan
3Department of Radiation Physics, Engineering, and Biology, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan
4Department of Radiology, Institute of Health Biosciences, University of Tokushima, Tokushima, Japan

Tóm tắt

The aim of this study was to select a suitable substrate candidate for dynamic nuclear polarization (DNP) studies and demonstrate its utility for evaluating intracellular metabolism. Hyperpolarized substances included 1-13C-pyruvate (Pyr), 1-13C-glucose (Glc), and 1-13C-acetate. A DNP polarizer and a 600-MHz vertical small-bore scanner were used for 13C-MR spectroscopic measurements. After polarization for 1 h, the dissolved solution was injected via a capillary line into the nuclear magnetic resonance tube in the scanner. The sequential spectra of the hyperpolarized 13C-labeled substrates were acquired in durations of more than 120 s, and a thermal spectrum was obtained more than 1 h thereafter. FM3A cancer cells of mammary tumors were cultured for intracellular detection of the hyperpolarized 13C-substances. The greatest sensitivity was found using Pyr with the longest T1 decay (51.5 s); and remarkably, the least sensitivity was observed using Glc with a signal decay of less than 2 s. An effective increase in sensitivity was shown using the other substances. The hyperpolarized intracellular study using 13C-Pyr showed distinct elevation of lactate levels. The DNP technique is useful for evaluating intracellular metabolism. However, Glc is not suitable for use with the DNP technique.

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Thông tin xuất bản

Radiation Medicine

Tập 28

173-179

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