Role of M Cells and Macrophages in the Entrance of Mycobacterium paratuberculosis into Domes of Ileal Peyer's Patches in Calves

Veterinary Pathology - Tập 25 Số 2 - Trang 131-137 - 1988
Eiichi Momotani1, Diana L. Whipple1, A B Thiermann1, Norman F. Cheville1
1US Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Ames, IA

Tóm tắt

Ligated ileal loops of calves were inoculated with live and heat-killed Mycobacterium paratuberculosis and were examined by light and electron microscopy. At 5 hours after inoculation, acid-fast bacilli were in subepithelial macrophages, but not in M cells covering domes. At 20 hours, more than 50 acid-fast bacilli per cross section were in subepithelial macrophages in domes. Both living and heat-killed bacilli passed into domes. Addition of anti- M. paratuberculosis bovine scrum to the inoculum enhanced entry of bacteria into domes. By electron microscopy, intact bacilli with electron-transparent zones (peribacillary spaces) were in the supranuclear cytoplasm of M cells at 20 hours. M cells also contained vacuoles, including electron-dense material interpreted as degraded bacilli. Subepithelial and intraepithelial macrophages contained bacilli and degraded bacterial material in phagosomes. These results suggest that calf ileal M cells take up bacilli, and that subepithelial and intraepithelial macrophages secondarily accept bacilli or bacterial debris which are expelled from M cells.

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Thông tin
Thông tin xuất bản

Veterinary Pathology

Tập 25 Số 2

131-137

Thông tin tác giả