Regulation of plant growth by cytokinin

Proceedings of the National Academy of Sciences of the United States of America - Tập 98 Số 18 - Trang 10487-10492 - 2001
Tomáš Werner1, Václav Motyka1, Miroslav Strnad1, Thomas Schmülling1
1Centre for Plant Molecular Biology (ZMBP)/Allgemeine Genetik, Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany; Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, CZ-16502 Prague 6, Czech Republic; and Palacký University and Institute of Experimental Botany ASCR, Department of Botany, Laboratory of Growth Regulators, S̆lechtitelů 11, CZ-78371 Olomouc, Czech Republic

Tóm tắt

Cytokinins are a class of plant-specific hormones that play a central role during the cell cycle and influence numerous developmental programs. Because of the lack of biosynthetic and signaling mutants, the regulatory roles of cytokinins are not well understood. We genetically engineered cytokinin oxidase expression in transgenic tobacco plants to reduce their endogenous cytokinin content. Cytokinin-deficient plants developed stunted shoots with smaller apical meristems. The plastochrone was prolonged, and leaf cell production was only 3–4% that of wild type, indicating an absolute requirement of cytokinins for leaf growth. In contrast, root meristems of transgenic plants were enlarged and gave rise to faster growing and more branched roots. These results suggest that cytokinins are an important regulatory factor of plant meristem activity and morphogenesis, with opposing roles in shoots and roots.

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Thông tin xuất bản

Proceedings of the National Academy of Sciences of the United States of America

Tập 98 Số 18

10487-10492

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