Regulation of Bcl-2 Family in TIP30-Induced Apoptosis in Hepatoblastoma Cells
Tóm tắt
Objective: To investigate the role of
TIP30 in the apoptotic signal pathway in HepG2, and Hep3B and Hu-7
hepatoblastoma cell lines. Methods: In order to
confirm whether TIP30 conducted Bcl-2 family was involved in
apoptosis signal pathway, MTT assay, in situ 3’ end labelling of
DNA assay and Western blot were carried out to detect the diverse
apoptotic function of TIP30 and the regulation of Bcl-2 family.
Results: TIP30 induced apoptosis as evidenced by
morphological changes in hepatoblastoma cells, which was
accompanied by up-regulating Bax and Bad proteins and stimulating
them from cytoplasm to mitochondria, and down-regulating Bcl-xl,
while it had no effect on the level of Bak protein.
Conclusion: TIP30 induced apoptosis partly by
modulating the protein levels of members of Bcl-2 family in
hepatoblastoma cells. Elucidating the mechanism by which TIP30
induces cell death might establish it as an anticancer factor.