Reelin is preferentially expressed in neurons synthesizing γ-aminobutyric acid in cortex and hippocampus of adult rats

Proceedings of the National Academy of Sciences of the United States of America - Tập 95 Số 6 - Trang 3221-3226 - 1998
Christine Pesold1, Francesco Impagnatiello1, Maria Giuseppina Pisu1, Doncho P. Uzunov1, E. Costa1, A Guidotti1, Héctor J. Caruncho1
1Psychiatric Institute, Department of Psychiatry, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612; and Department of Morphological Sciences, University of Santiango de Compostela School of Medicine, 15705 Santiago de Compostela, Spain

Tóm tắt

During embryonic development of brain laminated structures, the protein Reelin, secreted into the extracellular matrix of the cortex and hippocampus by Cajal–Retzius (CR) cells located in the marginal zone, contributes to the regulation of migration and positioning of cortical and hippocampal neurons that do not synthesize Reelin. Soon after birth, the CR cells decrease, and they virtually disappear during the following 3 weeks. Despite their disappearance, we can quantify Reelin mRNA (approximately 200 amol/μg of total RNA) and visualize it by in situ hybridization, and we detect the translated product of this mRNA by immunocytochemistry preferentially in γ-aminobutyric acid (GABA)ergic neurons of adult rat cortex and hippocampus. In adult rat cerebellum, Reelin is expressed in glutamatergic neurons (granule cells). The translated product of this mRNA is readily exported from the granule cell somata to the parallel fibers, where it has been detected by electron microscopy in axon terminals located presynaptically to Purkinje cell dendrites.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 95 Số 6

3221-3226

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