Recombinant expression of disulfide-rich proteins: carboxypeptidase inhibitors as model proteins
Tài liệu tham khảo
Arolas JL, Vendrell J, Aviles FX, Fricker LD: Metallocarboxypeptidases: emerging drug targets in biomedicine. Curr Pharm Des. 2006
Arolas JL, Lorenzo J, Rovira A, Castella J, Aviles FX, Sommerhoff CP: A carboxypeptidase inhibitor from the tick Rhipicephalus bursa : isolation, cDNA cloning, recombinant expression, and characterization. J Biol Chem. 2005, 280: 3441-3448. 10.1074/jbc.M411086200.
Peranen J, Rikkonen M, Hyvonen M, Kaariainen L: T7 vectors with modified T7lac promoter for expression of proteins in Escherichia coli . Anal Biochem. 1996, 236: 371-373. 10.1006/abio.1996.0187.
Molina MA, Aviles FX, Querol E: Expression of a synthetic gene encoding potato carboxypeptidase inhibitor using a bacterial secretion vector. Gene. 1992, 116: 129-138. 10.1016/0378-1119(92)90508-M.
Marino-Buslje C, Molina MA, Canals F, Aviles FX, Querol E: Overproduction of a recombinant carboxypeptidase inhibitor by optimization of fermentation conditions. Appl Microbiol Biotechnol. 1994, 41: 632-637.
Reverter D, Vendrell J, Canals F, J Horstmann, Aviles FX, Fritz H, Sommerhoff CP: A carboxypeptidase inhibitor from the medical leech Hirudo medicinalis. Isolation, sequence analysis, cDNA cloning, recombinant expression, and characterization. J Biol Chem. 1998, 273: 32927-32933. 10.1074/jbc.273.49.32927.