Recent Advances in Applications of Droplet Microfluidics

2014, Microfluidic platforms: A mainstream technology for the preparation of crystals, Chem. Soc. Rev., 43, 2253, 10.1039/C3CS60372E

Zhu, 2013, Analytical detection techniques for droplet microfluidics—A review, Anal. Chim. Acta, 787, 24, 10.1016/j.aca.2013.04.064

Samiei, 2015, Systematic analysis of geometrical based unequal droplet splitting in digital microfluidics, J. Micromech. Microeng., 25, 055008, 10.1088/0960-1317/25/5/055008

Oliveira, 2014, Kinetic study of glucose oxidase on microfluidic toner-based analytical devices for clinical diagnostics with image-based detection, Anal. Methods, 6, 4995, 10.1039/C4AY00260A

Jones, C.N., Hoang, A.N., Dimisko, L., Hamza, B., Martel, J., and Irimia, D. (2014). Microfluidic platform for measuring neutrophil chemotaxis from unprocessed whole blood. J. Vis. Exp.

Lopes, 2013, Bio-microfluidic platform for gold nanoprobe based DNA detection-application to mycobacterium tuberculosis, Biosens. Bioelectron., 48, 87, 10.1016/j.bios.2013.03.079

Peng, 2013, Single-pair fluorescence resonance energy transfer analysis of mRNA transcripts for highly sensitive gene expression profiling in near real time, Anal. Chem., 85, 7851, 10.1021/ac400729q

Vladisavljevic, 2013, Industrial lab-on-a-chip: Design, applications and scale-up for drug discovery and delivery, Adv. Drug Deliv. Rev., 65, 1626, 10.1016/j.addr.2013.07.017

Dressler, 2014, Droplet-based microfluidics: Enabling impact on drug discovery, J. Biomol. Screen., 19, 483, 10.1177/1087057113510401

Sun, 2013, Controlled dispensing and mixing of pico- to nanoliter volumes using on-demand droplet-based microfluidics, Microfluid. Nanofluid., 15, 117, 10.1007/s10404-012-1133-1

Lin, 2013, A microfluidic chip using phenol formaldehyde resin for uniform-sized polycaprolactone and chitosan microparticle generation, Molecules, 18, 6521, 10.3390/molecules18066521

Lin, 2013, Microfluidic synthesis of tail-shaped alginate microparticles using slow sedimentation, Electrophoresis, 34, 425, 10.1002/elps.201200282

Huang, 2013, A facile fabrication of alginate microbubbles using a gas foaming reaction, Molecules, 18, 9594, 10.3390/molecules18089594

Yang, 2014, One-step synthesis of platinum nanoparticles loaded in alginate bubbles, Nanoscale Res. Lett., 9, 277, 10.1186/1556-276X-9-277

Yang, 2014, Synthesis of uniform poly(d,l-lactide) and poly(d,l-lactide-co-glycolide) microspheres using a microfluidic chip for comparison, Electrophoresis, 35, 316, 10.1002/elps.201300185

Montanero, 2013, Building functional materials for health care and pharmacy from microfluidic principles and flow focusing, Adv. Drug Deliv. Rev., 65, 1447, 10.1016/j.addr.2013.08.003

Sarvothaman, 2015, Dynamic fluoroalkyl polyethylene glycol co-polymers: A new strategy for reducing protein adhesion in lab-on-a-chip devices, Adv. Funct. Mater., 25, 506, 10.1002/adfm.201402218

Seiffert, 2015, Faster droplet production by delayed surfactant-addition in two-phase microfluidics to form thermo-sensitive microgels, J. Colloid Interface Sci., 452, 38, 10.1016/j.jcis.2015.04.017

Pirbodaghi, 2015, Investigating the fluid dynamics of rapid processes within microfluidic devices using bright-field microscopy, Lab Chip, 15, 2140, 10.1039/C5LC00175G

Musterd, 2015, Calculating the volume of elongated bubbles and droplets in microchannels from a top view image, RSC Adv., 5, 16042, 10.1039/C4RA15163A

Zantow, M., Dendere, R., and Douglas, T.S. (2013). Image-based analysis of droplets in microfluidics. Eng. Med. Biol. Soc., 1776–1779.

Luo, 2013, Design of pin-constrained general-purpose digital microfluidic biochips, Comput. Aided Des. Integr. Circuits Syst., 32, 1307, 10.1109/TCAD.2013.2260192

Marian, 2015, Key factors for stable retention of fluorophores and labeled biomolecules in droplet-based microfluidics, Anal. Chem., 87, 2063, 10.1021/ac504736e

Pan, 2013, Optimization algorithms for the design of digital microfluidic biochips: A survey, Comput. Electr. Eng., 39, 112, 10.1016/j.compeleceng.2012.10.003

Lai, 2015, An intelligent digital microfluidic processor for biomedical detection, J. Sign Process Syst., 78, 85, 10.1007/s11265-014-0939-3

Huang, 2013, Reactant and waste minimization in multitarget sample preparation on digital microfluidic biochips, IEEE Trans. Comput. Aided Des. Integr. Circuits Syst., 32, 1484, 10.1109/TCAD.2013.2263035

Chen, 2013, A reliability-oriented placement algorithm for reconfigurable digital microfluidic biochips using 3-D deferred decision making technique, IEEE Trans. Comput. Aided Des. Integr. Circuits Syst., 32, 1151, 10.1109/TCAD.2013.2249558

Maftei, 2013, Module-based synthesis of digital microfluidic biochips with droplet-aware operation execution, ACM J. Emerg. Technol. Comput. Syst., 9, 21, 10.1145/2422094.2422096

Luo, 2013, Error recovery in cyberphysical digital microfluidic biochips, IEEE Trans. Comput. Aided Des. Integr. Circuits Syst., 32, 59, 10.1109/TCAD.2012.2211104

Isgor, 2015, Microfluidic droplet content detection using integrated capacitive sensors, Sens. Actuator B Chem., 210, 669, 10.1016/j.snb.2015.01.018

Lu, 2013, Detecting and tracking nosocomial methicillin-resistant staphylococcus aureus using a microfluidic SERS biosensor, Anal. Chem., 85, 2320, 10.1021/ac303279u

Kawano, Y., Otsuka, C., Sanzo, J., Higgins, C., Nirei, T., Schilling, T., and Ishikawa, T. (2015). Expanding imaging capabilities for microfluidics: Applicability of darkfield internal reflection illumination (DIRI) to observations in microfluidics. PLoS ONE, 10.

Kim, 2015, Optofluidic ultrahigh-throughput detection of fluorescent drops, Lab Chip, 15, 1417, 10.1039/C4LC01465K

Muluneh, 2014, Miniaturized, multiplexed readout of droplet-based microfluidic assays using time-domain modulation, Lab Chip, 14, 4638, 10.1039/C4LC00819G

Zhao, 2013, Lab-on-a-chip technologies for single-molecule studies, Lab Chip, 13, 2183, 10.1039/c3lc90042h

Cretich, 2015, Digital detection of biomarkers assisted by nanoparticles: Application to diagnostics, Trends Biotechnol., 33, 343, 10.1016/j.tibtech.2015.03.002

Streets, 2014, Microfluidics for biological measurements with single-molecule resolution, Curr. Opin. Biotechnol., 25, 69, 10.1016/j.copbio.2013.08.013

Kang, 2014, Droplet microfluidics for single-molecule and single-cell analysis in cancer research, diagnosis and therapy, TrAC Trends Anal. Chem., 58, 145, 10.1016/j.trac.2014.03.006

Oedit, 2015, Lab-on-a-chip hyphenation with mass spectrometry: Strategies for bioanalytical applications, Curr. Opin. Biotechnol., 31, 79, 10.1016/j.copbio.2014.08.009

Zeng, 2013, Quantitative microfluidic biomolecular analysis for systems biology and medicine, Anal. Bioanal. Chem., 405, 5743, 10.1007/s00216-013-6930-1

Choi, 2015, A droplet-based fluorescence polarization immunoassay (DFPIS) platform for rapid and quantitative analysis of biomarkers, Biosens. Bioelectron., 67, 497, 10.1016/j.bios.2014.09.013

Chen, 2015, A novel droplet dosing strategy-based versatile microscale biosensor for detection of DNA, protein and ion, Sens. Actuator B Chem., 215, 206, 10.1016/j.snb.2015.03.065

Khan, 2015, Specific detection of avidin-biotin binding using liquid crystal droplets, Colloid Surf. B Biointerfaces, 127, 241, 10.1016/j.colsurfb.2015.01.047

Kim, 2013, Glucose sensor using liquid-crystal droplets made by microfluidics, ACS Appl. Mater. Interfaces, 5, 13135, 10.1021/am404174n

Piao, 2015, Enzyme incorporated microfluidic device for in situ glucose detection in water-in-air microdroplets, Biosens. Bioelectron., 65, 220, 10.1016/j.bios.2014.10.032

Wu, 2014, Integrating bipolar electrochemistry and electrochemiluminescence imaging with microdroplets for chemical analysis, Biosens. Bioelectron., 53, 148, 10.1016/j.bios.2013.09.042

Zanoli, 2013, Peptide nucleic acid molecular beacons for the detection of pcr amplicons in droplet-based microfluidic devices, Anal. Bioanal. Chem., 405, 615, 10.1007/s00216-011-5638-3

Guan, 2014, A highly parallel microfluidic droplet method enabling single-molecule counting for digital enzyme detection, Biomicrofluidics, 8, 014110, 10.1063/1.4866766

Witters, 2013, Digital microfluidics-enabled single-molecule detection by printing and sealing single magnetic beads in femtoliter droplets, Lab Chip, 13, 2047, 10.1039/c3lc50119a

Benz, 2013, Protein-protein interaction analysis in single microfluidic droplets using fret and fluorescence lifetime detection, Lab Chip, 13, 2808, 10.1039/c3lc00057e

Rademeyer, 2015, Microfluidic system for high throughput characterisation of echogenic particles, Lab Chip, 15, 417, 10.1039/C4LC01206B

Duncanson, 2014, Microfluidic fabrication of perfluorohexane-shelled double emulsions for controlled loading and acoustic-triggered release of hydrophilic agents, Langmuir, 30, 13765, 10.1021/la502473w

Sheeran, 2015, Contrast-enhanced ultrasound imaging and in vivo circulatory kinetics with low-boiling-point nanoscale phase-change perfluorocarbon agents, Ultrasound Med. Biol., 41, 814, 10.1016/j.ultrasmedbio.2014.10.020

Duncanson, 2015, Microfluidic fabrication and micromechanics of permeable and impermeable elastomeric microbubbles, Langmuir, 31, 3489, 10.1021/la504843p

Dhanaliwala, 2013, Liquid flooded flow-focusing microfluidic device for in situ generation of monodisperse microbubbles, Microfluid. Nanofluid., 14, 457, 10.1007/s10404-012-1064-x

Neto, 2015, Benzothiadiazole derivatives as fluorescence imaging probes: Beyond classical scaffolds, Acc. Chem. Res., 48, 1560, 10.1021/ar500468p

Yu, 2015, On-demand one-step synthesis of monodisperse functional polymeric microspheres with droplet microfluidics, Langmuir, 31, 3982, 10.1021/acs.langmuir.5b00617

Wang, 2014, Functional polymeric microparticles engineered from controllable microfluidic emulsions, Acc. Chem. Res., 47, 373, 10.1021/ar4001263

Wang, 2013, Atom-economical in situ synthesis of BaSO4 as imaging contrast agents within poly(n-isopropylacrylamide) microgels using one-step droplet microfluidics, Green Chem., 15, 2222, 10.1039/c3gc40728d

Golberg, A., Linshiz, G., Kravets, I., Stawski, N., Hillson, N.J., Yarmush, M.L., Marks, R.S., and Konry, T. (2014). Cloud-enabled microscopy and droplet microfluidic platform for specific detection of escherichia coli in water. PLoS ONE, 9.

Park, 2015, Live cell imaging compatible immobilization of chlamydomonas reinhardtii in microfluidic platform for biodiesel research, Biotechnol. Bioeng., 112, 494, 10.1002/bit.25453

Seiffert, 2013, Small but smart: Sensitive microgel capsules, Angew. Chem. Int. Edit., 52, 11468, 10.1002/anie.201303055

Kaler, 2014, Droplet microfluidics for chip-based diagnostics, Sensors, 14, 23283, 10.3390/s141223283

Iwanaga, 2013, Facile fabrication of uniform size-controlled microparticles and potentiality for tandem drug delivery system of micro/nanoparticles, Colloid Surf. B Biointerfaces, 109, 301, 10.1016/j.colsurfb.2013.04.007

Pessi, 2014, Microfluidics-assisted engineering of polymeric microcapsules with high encapsulation efficiency for protein drug delivery, Int. J. Pharm., 472, 82, 10.1016/j.ijpharm.2014.06.012

Zhao, 2013, Multiphase flow microfluidics for the production of single or multiple emulsions for drug delivery, Adv. Drug Deliv. Rev., 65, 1420, 10.1016/j.addr.2013.05.009

Tasoglu, 2013, Manipulating biological agents and cells in micro-scale volumes for applications in medicine, Chem. Soc. Rev., 42, 5788, 10.1039/c3cs60042d

Rosenfeld, 2014, Review and analysis of performance metrics of droplet microfluidics systems, Microfluid. Nanofluid., 16, 921, 10.1007/s10404-013-1310-x

Mazutis, 2013, Single-cell analysis and sorting using droplet-based microfluidics, Nat. Protoc., 8, 870, 10.1038/nprot.2013.046

Rakszewska, 2014, One drop at a time: Toward droplet microfluidics as a versatile tool for single-cell analysis, NPG Asia Mater., 6, e133, 10.1038/am.2014.86

Schlicht, 2015, Droplet-interface-bilayer assays in microfluidic passive networks, Sci. Rep., 5, 9951, 10.1038/srep09951

Cao, 2015, Droplet-based microfluidics for microtoxicological studies, Eng. Life Sci., 15, 306, 10.1002/elsc.201400074

Au, 2013, Cellular bias on the microscale: Probing the effects of digital microfluidic actuation on mammalian cell health, fitness and phenotype, Integr. Biol., 5, 1014, 10.1039/c3ib40104a

Arriaga, 2014, Microfluidic assembly of multistage porous silicon-lipid vesicles for controlled drug release, Lab Chip, 14, 1083, 10.1039/C3LC51260F

Huang, 2014, Generation of uniform polymer eccentric and core-centered hollow microcapsules for ultrasound-regulated drug release, J. Mater. Chem. B, 2, 6848, 10.1039/C4TB01050G

Huang, 2014, Synthesis of uniform core-shell gelatin-alginate microparticles as intestine-released oral delivery drug carrier, Electrophoresis, 35, 336, 10.1002/elps.201300194

Li, 2015, Controllable microfluidic fabrication of janus and microcapsule particles for drug delivery applications, RSC Adv., 5, 23181, 10.1039/C4RA17153E

Yang, 2014, Core-shell structure microcapsules with dual pH-responsive drug release function, Electrophoresis, 35, 2680, 10.1002/elps.201400210

Marquis, 2015, Microfluidics assisted generation of innovative polysaccharide hydrogel microparticles, Carbohydr. Polym., 116, 189, 10.1016/j.carbpol.2014.01.083

Xue, 2014, Microfluidic synthesis of monodisperse pegda microbeads for sustained release of 5-fluorouracil, Microfluid. Nanofluid., 18, 333, 10.1007/s10404-014-1436-5

Velasco, 2014, Chitosan/agarose hydrogels: Cooperative properties and microfluidic preparation, Carbohydr. Polym., 111, 348, 10.1016/j.carbpol.2014.04.087

Kong, 2014, Microfluidics fabrication of monodisperse biocompatible phospholipid vesicles for encapsulation and delivery of hydrophilic drug or active compound, Langmuir, 30, 3905, 10.1021/la404201m

Shim, 2013, Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays, ACS Nano, 7, 5955, 10.1021/nn401661d

Wagli, 2013, Microfluidic droplet-based liquid-liquid extraction and on-chip IR spectroscopy detection of cocaine in human saliva, Anal. Chem., 85, 7558, 10.1021/ac401606p

Hugo, 2014, A centrifugal microfluidic platform for point-of-care diagnostic applications, S. Afr. J. Sci., 110, 1, 10.1590/sajs.2014/20130091

Norian, 2014, An integrated cmos quantitative-polymerase-chain-reaction lab-on-chip for point-of-care diagnostics, Lab Chip, 14, 4076, 10.1039/C4LC00443D

Golberg, 2013, Picoliter droplet microfluidic immunosorbent platform for point-of-care diagnostics of tetanus, Microchim. Acta, 180, 855, 10.1007/s00604-013-0998-3

Bardiya, 2014, Analysis of single nucleotide polymorphism in human angiogenin using droplet-based microfluidics, BioChip J., 8, 15, 10.1007/s13206-014-8103-5

Kim, 2015, Magnetic bead droplet immunoassay of oligomer amyloid beta for the diagnosis of alzheimer’s disease using micro-pillars to enhance the stability of the oil-water interface, Biosens. Bioelectron., 67, 724, 10.1016/j.bios.2014.10.042

Prakash, 2014, Droplet microfluidic chip based nucleic acid amplification and real-time detection of influenza viruses, J. Electrochem. Soc., 161, B3083, 10.1149/2.013402jes

Prakash, 2015, Multiplex, quantitative, reverse transcription pcr detection of influenza viruses using droplet microfluidic technology, Micromachines, 6, 63, 10.3390/mi6010063

Rosenfeld, L., Cheng, Y.F., Rao, J.H., and Tang, S.K.Y. (2014). Rapid detection of tuberculosis using droplet-based microfluidics. Proc. SPIE 8976 Microfluid. BioMEMS Med. Microsyst. XII.

Chiu, 2015, Development of a microfluidic-based optical sensing device for label-free detection of circulating tumor cells (CTCs) through their lactic acid metabolism, Sensors, 15, 6789, 10.3390/s150306789

Chen, 2015, Assembly-line manipulation of droplets in microfluidic platform for fluorescence encoding and simultaneous multiplexed DNA detection, Talanta, 134, 271, 10.1016/j.talanta.2014.11.027

Ng, 2015, Digital microfluidic platform for the detection of rubella infection and immunity: A proof of concept, Clin. Chem., 61, 420, 10.1373/clinchem.2014.232181

Wu, 2014, Rapid and reproducible analysis of thiocyanate in real human serum and saliva using a droplet sers-microfluidic chip, Biosens. Bioelectron., 62, 13, 10.1016/j.bios.2014.06.026

He, 2014, A novel microbead-based microfluidic device for rapid bacterial identification and antibiotic susceptibility testing, Eur. J. Clin. Microbiol. Infect. Dis., 33, 2223, 10.1007/s10096-014-2182-z

Wang, 2014, Rapid detection of bacteriophages in starter culture using water-in-oil-in-water emulsion microdroplets, Appl. Microbiol. Biotechnol., 98, 8347, 10.1007/s00253-014-6018-7

Tjhung, 2014, Rapid enumeration of phage in monodisperse emulsions, Anal. Chem., 86, 5642, 10.1021/ac500244g

Li, 2014, Highly sensitive and homogeneous detection of membrane protein on a single living cell by aptamer and nicking enzyme assisted signal amplification based on microfluidic droplets, Anal. Chem., 86, 5101, 10.1021/ac500881p

Huang, 2014, A biocompatible open-surface droplet manipulation platform for detection of multi-nucleotide polymorphism, Lab Chip, 14, 2057, 10.1039/C4LC00089G

Chiou, 2013, Topography-assisted electromagnetic platform for blood-to-pcr in a droplet, Biosens. Bioelectron., 50, 91, 10.1016/j.bios.2013.06.011

Shuga, J., Zeng, Y., Novak, R., Lan, Q., Tang, X., Rothman, N., Vermeulen, R., Li, L., Hubbard, A., and Zhang, L. (2013). Single molecule quantitation and sequencing of rare translocations using microfluidic nested digital pcr. Nucleic Acids. Res.

Hu, 2015, Versatile microfluidic droplets array for bioanalysis, ACS Appl. Mater. Interfaces, 7, 935, 10.1021/am5075216

Wang, 2014, Mixed hydrogel bead-based tumor spheroid formation and anticancer drug testing, Analyst, 139, 2449, 10.1039/C4AN00015C

Du, 2013, Cell-based drug combination screening with a microfluidic droplet array system, Anal. Chem., 85, 6740, 10.1021/ac400688f

Kumar, 2015, Digital microfluidics for time-resolved cytotoxicity studies on single non-adherent yeast cells, Lab Chip, 15, 1852, 10.1039/C4LC01469C

Cho, 2013, Droplet-based microfluidic platform for high-throughput, multi-parameter screening of photosensitizer activity, Anal. Chem., 85, 8866, 10.1021/ac4022067

Wetzel, 2015, Changing growth behavior of heavy-metal tolerant bacteria: Media optimization using droplet-based microfluidics, Eng. Life Sci., 15, 327, 10.1002/elsc.201400230

Yamashita, 2015, Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device, J. Biosci. Bioeng., 119, 492, 10.1016/j.jbiosc.2014.09.018

Zang, 2013, Real-time image processing for label-free enrichment of actinobacteria cultivated in picoliterliter droplets, Lab Chip, 13, 3707, 10.1039/c3lc50572c

Pyne, D.G., Liu, J., Abdelgawad, M., and Sun, Y. (2014). Digital microfluidic processing of mammalian embryos for vitrification. PLoS ONE, 9.

Geng, 2014, Single-cell forensic short tandem repeat typing within microfluidic droplets, Anal. Chem., 86, 703, 10.1021/ac403137h

Akbari, 2014, Microfluidic encapsulation of cells in alginate particles via an improved internal gelation approach, Microfluid. Nanofluid., 16, 773, 10.1007/s10404-013-1264-z

Akbari, 2014, A droplet-based heterogeneous immunoassay for screening single cells secreting antigen-specific antibodies, Lab Chip, 14, 3275, 10.1039/C4LC00082J

Lim, 2013, Ultra-high throughput detection of single cell β-galactosidase activity in droplets using micro-optical lens array, Appl. Phys. Lett., 103, 203704, 10.1063/1.4830046

Najah, 2013, New glycosidase substrates for droplet-based microfluidic screening, Anal. Chem., 85, 9807, 10.1021/ac4022709

Polenz, 2015, Monitoring reactive microencapsulation dynamics using microfluidics, Soft Matter, 11, 2916, 10.1039/C5SM00218D

Shim, 2013, Elaborate design strategies toward novel microcarriers for controlled encapsulation and release, Part. Part. Syst. Charact., 30, 9, 10.1002/ppsc.201200044

Seiffert, 2013, Microgel capsules tailored by droplet-based microfluidics, Chem. Phys. Chem., 14, 295, 10.1002/cphc.201200749

Tang, 2015, In situ microfluidic fabrication of multi-shape inorganic/organic hybrid particles with controllable surface texture and porous internal structure, RSC Adv., 5, 12872, 10.1039/C4RA11492B

Yan, 2014, Formation of monodisperse silica microparticles with various shapes and surface morphologies using double emulsion templates, Colloid Surf. A Physicochem. Eng. Asp., 443, 88, 10.1016/j.colsurfa.2013.10.049

Maan, 2015, Microfluidic emulsification in food processing, J. Food Eng., 147, 1, 10.1016/j.jfoodeng.2014.09.021

Shewan, 2013, Review of techniques to manufacture micro-hydrogel particles for the food industry and their applications, J. Food Eng., 119, 781, 10.1016/j.jfoodeng.2013.06.046