Psychrotrophic yeast Yarrowia lipolytica NCYC 789 mediates the synthesis of antimicrobial silver nanoparticles via cell-associated melanin

AMB Express - Tập 3 Số 1 - 2013
Mugdha Apte1, Devashree Sambre1, Shital Gaikawad1, Suresh G. Joshi1, Ashok Bankar1, Anuj Kumar1, Smita Zinjarde1
1Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, India

Tóm tắt

Abstract A psychrotrophic marine strain of the ascomycetous yeast Yarrowia lipolytica (NCYC 789) synthesized silver nanoparticles (AgNPs) in a cell-associated manner. These nanostructures were characterized by UV-Visible spectroscopy and scanning electron microscope-energy dispersive spectrometer (SEM-EDS) analysis. The brown pigment (melanin) involved in metal-interactions was obtained from the cells. This extracted pigment also mediated the synthesis of silver nanoparticles that were characterized by a variety of analytical techniques. The melanin-derived nanoparticles displayed antibiofilm activity. This paper thus reports the synthesis of AgNPs by the biotechnologically important yeast Y. lipolytica; proposes a possible mechanism involved in the synthetic process and describes the use of the bio-inspired nanoparticles as antibiofilm agents.

Từ khóa


Tài liệu tham khảo

Agnihotri M, Joshi S, Kumar AR, Zinjarde S, Kulkarni S: Biosynthesis of gold nanoparticles by the tropical marine yeast Yarrowia lipolytica NCIM 3589. Mater Lett 2009, 63: 1231–1234. 10.1016/j.matlet.2009.02.042

Apte M, Girme G, Nair R, Bankar A, Kumar AR, Zinjarde S: Melanin mediated synthesis of gold nanoparticles by Yarrowia lipolytica . Mater Lett 2013, 95: 149–152.

Bankar AV, Kumar AR, Zinjarde SS: Environmental and industrial applications of Yarrowia lipolytica . Appl Microbiol Biotechnol 2009, 84: 847–865. 10.1007/s00253-009-2156-8

Bankar AV, Joshi BS, Kumar AR, Zinjarde SS: Banana peel extract mediated synthesis of gold nanoparticles. Colloids Surf B 2010, 80: 45–50. 10.1016/j.colsurfb.2010.05.029

Bankar A, Joshi B, Kumar AR, Zinjarde S: Banana peel extract mediated novel route for the synthesis of silver nanoparticles. Colloids Surf A 2010, 368: 58–63. 10.1016/j.colsurfa.2010.07.024

Beopoulos A, Desfougeres T, Sabirova J, Zinjarde S, Nicaud JM: The hydrocarbon degrading oleaginous yeast Yarrowia lipolytica . In Handbook of hydrocarbons and lipid biology. Edited by: Timmis KN. Heidelberg: Springer-Verlag Berlin; 2010:2111–2121.

Carreira A, Ferreira LM, Loureiro V: Production of brown tyrosine pigments by the yeast Yarrowia lipolytica . J Appl Microbiol 2001, 90: 372–379. 10.1046/j.1365-2672.2001.01256.x

Carreira A, Paloma L, Loureiro V: Pigment producing yeasts involved in the brown surface discoloration of ewes’ cheese. Int J Food Microbiol 1998, 41: 223–230. 10.1016/S0168-1605(98)00054-3

Castro-Longoria E, Vilchis-Nestor AR, Avalos-Borja M: Biosynthesis of silver, gold and bimetallic nanoparticles using the filamentous fungus Neurospora crassa . Colloids Surf B 2011, 83: 42–48. 10.1016/j.colsurfb.2010.10.035

Commoner B, Townsend J, Pike GE: Free radicals in biological materials. Nature 1954, 174: 689–691. 10.1038/174689a0

Doering TL, Nosanchuk JD, Roberts WK, Casadevall A: Melanin as a potential cryptococcal defense against microbicidal proteins. Med Mycol 1999, 37: 175–181.

Du L, Xian L, Feng JX: Rapid extra-/intracellular biosynthesis of gold nanoparticles by the fungus Penicillium sp. J Nanopart Res 2010, 13: 921–930.

Duran N, Marcato PD, Duran M, Yadav A, Gade A, Rai M: Mechanistic aspects in the biogenic synthesis of extracellular metal nanoparticles by peptides, bacteria, fungi, and plants. App Microbiol Biotechnol 2011, 90: 1609–1624. 10.1007/s00253-011-3249-8

Dusane DH, Pawar VS, Nancharaiah YV, Venugopalan VP, Kumar AR, Zinjarde SS: Anti-biofilm potential of a glycolipid surfactant produced by a tropical marine strain of Serratia marcescens . Biofouling 2011, 27: 645–654. 10.1080/08927014.2011.594883

Feller G, Gerday C: Psychrophilic enzymes: hot topics in cold adaptation. Nat Rev Microbiol 2003, 1: 200–208. 10.1038/nrmicro773

Fickers P, Benetti PH, Wache´ Y, Marty A, Mauersberger S, Smit MS, Nicaud JM: Hydrophobic substrate utilization by the yeast Yarrowia lipolytica , and its potential applications. FEMS Yeast Res 2005, 5: 527–543. 10.1016/j.femsyr.2004.09.004

Fogarty RV, Tobin JM: Fungal melanins and their interactions with metals. Enz Microb Technol 1996, 19: 311–317. 10.1016/0141-0229(96)00002-6

Gade AK, Bonde P, Ingle AP, Marcato PD, Duran N, Rai MK: Exploitation of Aspergillus niger for synthesis of silver nanoparticles. J Biobased Mater Bioener 2008, 2: 243–247. 10.1166/jbmb.2008.401

Gade A, Ingle A, Whiteley C, Rai M: Mycogenic metal nanoparticles: progress and applications. Biotechnol Lett 2010, 32: 593–600. 10.1007/s10529-009-0197-9

Gardini F, Tofalo R, Belletti N, Iucci L, Suzzi G, Torriani S, Guerzoni ME, Lanciotti R: Characterization of yeasts involved in the ripening of Pecorino Crotonese cheese. Food Microbiol 2006, 23: 641–648. 10.1016/j.fm.2005.12.005

He R, Qian X, Yin J, Zhu Z: Formation of silver dendrites under microwave irradiation. Chem Phys Lett 2003, 369: 454–458. 10.1016/S0009-2614(02)02036-5

Hoiby N, Bjarnsholt T, Givskov M, Molin S, Ciofu O: Antibiotic resistance of bacterial biofilms. Int J Antimicrob Agents 2010, 35: 322–332. 10.1016/j.ijantimicag.2009.12.011

Horak V, Gillette JR: A study of the oxidation-reduction state of synthetic 3, 4-dihydroxy-DL-phenylalanine melanin. Mol Pharm 1971, 7: 429–433.

Ito H, Inouhe M, Tohoyama H, Joho M: Characteristics of copper tolerance in Yarrowia lipolytica . Biometals 2007, 20: 773–780. 10.1007/s10534-006-9040-0

Jacobson ES: Pathogenic roles for fungal melanins. Clin Microbiol Rev 2000, 13: 708–717. 10.1128/CMR.13.4.708-717.2000

Jain J, Arora S, Rajwade JM, Omray P, Khandelwal S, Paknikar KM: Silver nanoparticles in therapeutics: development of an antimicrobial gel formulation for topical use. Mol Pharm 2009, 6: 1388–1401. 10.1021/mp900056g

Kalishwaralal K, Manikanth SB, Pandian SRK, Deepak V, Gurunathan S: Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis . Colloids Surf B 2010, 79: 340–344. 10.1016/j.colsurfb.2010.04.014

Kim SW, Baek Y-W, An Y-J: Assay-dependent effect of silver nanoparticles to Escherichia coli and Bacillus subtilis . Appl Microbiol Biotechnol 2011, 92: 1045–1052. 10.1007/s00253-011-3611-x

Kumar GC, Mamidyala SK: Extracellular synthesis of silver nanoparticles using culture supernatant of Pseudomonas aeruginosa . Colloids Surf B 2011, 84: 462–466. 10.1016/j.colsurfb.2011.01.042

Lanciotti R, Vannini L, Lopez CC, Gobbetti M, Guerzoni ME: Evaluation of the ability of Yarrowia lipolytica to impart strain-dependent characteristics to cheese when used as a ripening adjunct. Int J Dairy Technol 2005, 58: 89–99. 10.1111/j.1471-0307.2005.00197.x

Margesin R, Gander S, Zacke G, Gounot AM, Schinner F: Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. Extremophiles 2003, 7: 451–458. 10.1007/s00792-003-0347-2

Rai M, Yadav A, Gade A: Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 2009, 27: 76–83. 10.1016/j.biotechadv.2008.09.002

Ravindra S, Mohan YM, Reddy NN, Raju KM: Fabrication of antibacterial cotton fibres loaded with silver nanoparticles via “Green Approach”. Colloids Surf A 2010, 367: 31–40. 10.1016/j.colsurfa.2010.06.013

Rodrigues AG, Ping LY, Marcato PD, Alves OL, Silva MCP, Ruiz RC, Melo IS, Tasic L, De Souza AO: Biogenic antimicrobial silver nanoparticles produced by fungi. Appl Microbiol Biotechnol 2013, 97: 775–782. 10.1007/s00253-012-4209-7

Ross SS, Morris EO: An investigation of the yeast flora of marine fish from Scottish coastal waters and a fishing ground off Iceland. J Appl Microbiol 1965, 28: 224–234. 10.1111/j.1365-2672.1965.tb02147.x

Rywińska A, Rymowicz W: High-yield production of citric acid by Yarrowia lipolytica on glycerol in repeated-batch bioreactors. J Ind Microbiol Biotechnol 2010, 37: 431–443. 10.1007/s10295-009-0687-8

Sa´nchez LA, Go´mez FF, Delgado OD: Cold-adapted microorganisms as a source of new antimicrobials. Extremophiles 2009, 13: 111–120. 10.1007/s00792-008-0203-5

Sharma VK, Yngard RA, Lin Y: Silver nanoparticles: Green synthesis and their antimicrobial activities. Adv Colloids Interface Sci 2009, 145: 83–96. 10.1016/j.cis.2008.09.002

Shivaji S, Madhu S, Singh S: Extracellular synthesis of antibacterial silver nanoparticles using psychrophilic bacteria. Process Biochem 2011, 46: 1800–1807. 10.1016/j.procbio.2011.06.008

Sintubin L, Windt WD, Dick J, Mast J, van der Ha D, Verstraete W, Boon N: Lactic acid bacteria as reducing and capping agent for the fast and efficient production of silver nanoparticles. Appl Microbiol Biotechnol 2009, 84: 741–749. 10.1007/s00253-009-2032-6

Sivaraman SK, Elango I, Kumar S, Santhanam V: A green protocol for room temperature synthesis of silver nanoparticles in seconds. Curr Sci 2009, 97: 1055–1059.

Suresh AK, Pelletier DA, Wang W, Moon JW, Gu B, Mortensen NP, Allison DP, Joy DC, Phelps TJ, Doktycz MJ: Silver nanocrystallites: biofabrication using Shewanella oneidensis , and an evaluation of their comparative toxicity on Gram-negative and Gram-positive bacteria. Env Sci Technol 2010, 44: 5210–5215. 10.1021/es903684r

Tang S, Meng X, Lu H, Zhu S: PVP-assisted sonoelectrochemical growth of silver nanostructures with various shapes. Mater Chem Phys 2009, 116: 464–468. 10.1016/j.matchemphys.2009.04.004

Thakkar KN, Mhatre SS, Parikh RY: Biological synthesis of metallic nanoparticles. Nanomed: Nanotechnol Biol Med 2010, 6: 257–262. 10.1016/j.nano.2009.07.002

Tomaszewska L, Rywińska A, Gładkowski W: Production of erythritol and mannitol by Yarrowia lipolytica yeast in media containing glycerol. J Ind Microbiol Biotechnol 2012, 39: 1333–1343. 10.1007/s10295-012-1145-6

Veerapandian M, Yun K: Functionalization of biomolecules on nanoparticles: specialized for antibacterial applications. Appl Microbiol Biotechnol 2011, 90: 1655–1667. 10.1007/s00253-011-3291-6

Vigneshwaran N, Kathe AA, Varadarajan PV, Nachane RP, Balasubramanya RH: Biomimetics of silver nanoparticles by white rot Phaenerochaete chrysosporium . Colloids Surf B 2006, 53: 55–59. 10.1016/j.colsurfb.2006.07.014

Zhou Y, Kong Y, Kundu S, Cirillo JD, Liang H: Antibacterial activities of gold and silver nanoparticles against Escherichia coli and bacillus Calmette-Guérin. J Nanobiotechnol 2012, 10: 19. 10.1186/1477-3155-10-19

Zinjarde SS: Bio-inspired nanomaterials and their applications as antimicrobial agents. Chronicles of Young Scientists 2012, 3: 74–81. 10.4103/2229-5186.94314